A Proteome Reference Map and Proteomic Analysis of Bifidobacterium longum NCC2705

Yuan, Jing; Zhu, Li; Liu, Xiankai; Li, Ting; Zhang, Ying; Ying, Tianyi; Wang, Bin; Wang, Junjun; Dong, Hua; Feng, Erling; Li, Qiang; Wang, Jie; Wang, Hongxia; Wei, Kaihua; Zhang, Xuemin; Huang, Cuifeng; Huang, Peitang; Huang, Liuyu; Zeng, Ming; Wang, Hengliang

doi:10.1074/mcp.m500410-mcp200

Cited by 90 publications

(93 citation statements)

References 65 publications

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“…To unravel the molecular mechanisms leading to bile adaptation, high-throughput molecular techniques are being widely used as a first approach to understand the physiological changes involved in this process (7,8,29,30,57). Here, we used proteomics to detect target proteins affected by growth in the presence of bile in B. animalis subsp lactis.…”

Section: Resultsmentioning

confidence: 99%

Adaptation and Response of Bifidobacterium animalis subsp. lactis to Bile: a Proteomic and Physiological Approach

Sánchez

Champomier‐Vergès²,

Stuer‐Lauridsen

et al. 2007

Appl Environ Microbiol

114

113

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Bile salts are natural detergents that facilitate the digestion and absorption of the hydrophobic components of the diet. However, their amphiphilic nature makes them very inhibitory for bacteria and strongly influences bacterial survival in the gastrointestinal tract. Adaptation to and tolerance of bile stress is therefore crucial for the persistence of bacteria in the human colonic niche. Bifidobacterium animalis subsp. lactis, a probiotic bacterium with documented health benefits, is applied largely in fermented dairy products. In this study, the effect of bile salts on proteomes of B. animalis subsp. lactis IPLA 4549 and its bile-resistant derivative B. animalis subsp. lactis 4549dOx was analyzed, leading to the identification of proteins which may represent the targets of bile salt response and adaptation in B. animalis subsp. lactis. The comparison of the wild-type and the bile-resistant strain responses allowed us to hypothesize about the resistance mechanisms acquired by the derivative resistant strain and about the bile salt response in B. animalis subsp. lactis. In addition, significant differences in the levels of metabolic end products of the bifid shunt and in the redox status of the cells were also detected, which correlate with some differences observed between the proteomes. These results indicate that adaptation and response to bile in B. animalis subsp. lactis involve several physiological mechanisms that are jointly dedicated to reduce the deleterious impact of bile on the cell's physiology.

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Section: Resultsmentioning

confidence: 99%

Adaptation and Response of Bifidobacterium animalis subsp. lactis to Bile: a Proteomic and Physiological Approach

Sánchez

Champomier‐Vergès²,

Stuer‐Lauridsen

et al. 2007

Appl Environ Microbiol

114

113

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“…While ABC transporters are the most popular transport system for sugars in bifidobacteria, there is little functional knowledge of these transporters which is specific to these bacteria. A proteomic analysis of B. longum showed that a fructose-specific ABC sugar-binding protein (BL0033) and an ATPase (BL0034) were highly produced in the presence of fructose compared to those in the presence of glucose, suggesting that this ABC-type transporter is indeed fructose specific and may play an important role in the uptake of fructose (353). In addition to sugar uptake, ABC-type transporters can also function in efflux of cytotoxic compounds out of the cell.…”

Section: Metabolism Of Simple Sugarsmentioning

confidence: 99%

Genomic Insights into Bifidobacteria

Lee

O’Sullivan

2010

Microbiol Mol Biol Rev

243

197

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SUMMARY Since the discovery in 1899 of bifidobacteria as numerically dominant microbes in the feces of breast-fed infants, there have been numerous studies addressing their role in modulating gut microflora as well as their other potential health benefits. Because of this, they are frequently incorporated into foods as probiotic cultures. An understanding of their full interactions with intestinal microbes and the host is needed to scientifically validate any health benefits they may afford. Recently, the genome sequences of nine strains representing four species of Bifidobacterium became available. A comparative genome analysis of these genomes reveals a likely efficient capacity to adapt to their habitats, with B. longum subsp. infantis exhibiting more genomic potential to utilize human milk oligosaccharides, consistent with its habitat in the infant gut. Conversely, B. longum subsp. longum exhibits a higher genomic potential for utilization of plant-derived complex carbohydrates and polyols, consistent with its habitat in an adult gut. An intriguing observation is the loss of much of this genome potential when strains are adapted to pure culture environments, as highlighted by the genomes of B. animalis subsp. lactis strains, which exhibit the least potential for a gut habitat and are believed to have evolved from the B. animalis species during adaptation to dairy fermentation environments.

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“…B. longum maintains human gastrointestinal tract balance and is used as a probiotic in various dairy products (Lin and Chang, 2000;Šrůtková et al, 2011). It improves lactose tolerance and has been used to diarrhea and food allergies (Schell et al, 2002;Yuan et al, 2006). In addition, B. longum has been reported to arteriosclerosis and stroke (Lin and Chang, 2000).…”

Section: Discussionmentioning

confidence: 99%

Antiviral Properties of Probiotic Mixtures against Rotavirus in the Rat

Park¹,

Lee²,

Kim³

et al. 2014

The Korean Journal of Microbiology

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Rotavirus is a major cause of acute gastroenteritis in young children in developed and developing countries. The use of probiotics for the treatment of gastrointestinal diseases is both safe and easily accessible. In this study, we evaluated the anti-rotaviral activities of probiotic mixtures in a Sprague-Dawley rat. 24 litters with their dams were randomly assigned to four groups; placebo, phosphate buffered saline (PBS), and two probiotic mixture (PRO-1 and PRO-2) groups. All rats were inoculated with rotavirus at dose of 8 log plaque forming units per rat at 5 days old. Animals in the PRO-1 and PRO-2 groups were orally administered probiotic mixtures 1 or 2, respectively, at a dose of 8 log colony forming units daily during 4 days. For control purposes, placebo and PBS groups were orally administered the same amount of placebo (containing maltose and polydextrose) or PBS once daily for 4 days, respectively. Antiviral analysis was performed by real-time quantitative PCR (RT-qPCR) and observing intestinal villi. As a result, weights of small intestines were greater in the PRO-1, PRO-2 groups than in control groups. Villi were short and villous epithelial necrosis was exhibited in control groups, but these morphological changes were not observed in PRO-1, PRO-2 treated rats. RT-qPCR analysis showed that VP7 gene level of rotavirus in fecal samples and small intestinal epithelial cells were lower in the PRO-1 and PRO-2 groups. These findings suggest that probiotic mixtures may be useful probiotics for the treatment of or as alternative therapies for rotaviral gastroenteritis.

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A Proteome Reference Map and Proteomic Analysis of Bifidobacterium longum NCC2705

Cited by 90 publications

References 65 publications

Adaptation and Response of Bifidobacterium animalis subsp. lactis to Bile: a Proteomic and Physiological Approach

Adaptation and Response of Bifidobacterium animalis subsp. lactis to Bile: a Proteomic and Physiological Approach

Genomic Insights into Bifidobacteria

Antiviral Properties of Probiotic Mixtures against Rotavirus in the Rat

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