A Protein Nanocarrier from Charge-Conversion Polymer in Response to Endosomal pH

Lee, Yan; Fukushima, Shigeto; Bae, Younsoo; Hiki, Shigehiro; Ishii, Takehiko; Kataoka, Kazunori

doi:10.1021/ja071090b

Cited by 378 publications

(307 citation statements)

References 13 publications

(15 reference statements)

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“…This high electropositivity of D/PSP at pH 5.0 could contribute to its endo-lysosomal escape. [44][45][46] As a control nanoparticle, PP was fabricated by polymerizing N-isopropylacrylamide in the presence of bPEI. In the same manner, DOX was physically encapsulated in PP (form "D/PP") and its payload was 15.1%.…”

Section: Fabrication and Characterization Of D/psp@m/gnpmentioning

confidence: 99%

A functional nanocarrier that copenetrates extracellular matrix and multiple layers of tumor cells for sequential and deep tumor autophagy inhibitor and chemotherapeutic delivery

et al. 2016

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To further enhance the intensity of deep tumor drug delivery and integrate a combined therapy, we herein report on a core-shell nanocarrier that could simultaneously overcome the double barriers of the extracellular matrix (ECM) and multiple layers of tumor cells (MLTC). A pH-triggered reversible swelling-shrinking core and an MMP2 (matrix metallopeptidase 2) degradable shell were developed to encapsulate chemotherapeutics and macroautophagy/autophagy inhibitors, respectively. MMP2 degraded the shell, which was followed by the autophagy inhibitors' release. The exposed core could diffuse along the pore within the ECM to deliver chemotherapeutics into deep tumors, and it was able to swell in lysosomes and shrink back in the cytoplasm or ECM. The swelling of the core resulted in the rapid release of chemotherapeutics to kill autophagy-inhibited cells. After leaving the dead cells, the shrinking core could act on neighboring cells that were closer to the center of the tumor. The core thus could also cross MLTC layer by layer to deliver chemotherapeutics into the deep tumor.

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Section: Fabrication and Characterization Of D/psp@m/gnpmentioning

confidence: 99%

A functional nanocarrier that copenetrates extracellular matrix and multiple layers of tumor cells for sequential and deep tumor autophagy inhibitor and chemotherapeutic delivery

et al. 2016

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“…Thus, after endocytosis of these polyplexes, the ethylenediamine unit in the block copolymer is expected to facilitate the efficient translocation of the micelle from the late endosomes toward the cytoplasm. Moreover, Lee et al prepared PIC micelles from PEG-b-poly[(N -citraconyl-2-aminoethyl)aspartamide] (PEG-b-P(Asp(EDA-Cit))) with the ability to switch the charge from anionic to cationic at the endosomal pH due to the degradation of the citraconic amide side chain at pH 5.5 [27,28]. This strategy is very promising for loading a wide range of charged compounds, while controlling the stability, charge and biological fate of the nanoassemblies.…”

Section: Endogenous Triggersmentioning

confidence: 99%

Multifunctional nanoassemblies of block copolymers for future cancer therapy

Cabral

Kataoka

2010

Science and Technology of Advanced Materials

Self Cite

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Nanoassemblies from amphiphilic block copolymers are promising nanomedicine platforms for cancer diagnosis and therapy due to their relatively small size, high loading capacity of drugs, controlled drug release, in vivo stability and prolonged blood circulation. Recent clinical trials with self-assembled polymeric micelles incorporating anticancer drugs have shown improved antitumor activity and decreased side effects encouraging the further development of nanoassemblies for drug delivery. This review summarizes recent approaches considering stimuli-responsive, multifunctionality and more advanced architectures, such as vesicles or worm-like micelles, for tumor-specific drug and gene delivery.

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“…Kataoka's group introduced a charge conversion polymer, specifically PEG-b-poly(N′-citraconyl-2-aminoethly-aspartamide) [PEG-b-pAsp(EDA-Cit)], in order to fabricate a novel protein drug carrier for cytosolic delivery [39,40]. The polyionic complex (PIC) was prepared in an aqueous solution via the electrostatic interaction between a charge conversion polymer and a cationic protein (lysozyme).…”

Section: Protein Drug Deliverymentioning

confidence: 99%

Intelligent Polymeric Nanocarriers Responding to Physical or Biological Signals: A New Paradigm of Cytosolic Drug Delivery for Tumor Treatment

Lee

Baik

et al. 2010

Polymers

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Abstract:The physicochemical properties of stimuli-responsive polymers change with physical or biological signals, such as pH, enzyme concentrations, and temperature. These polymers have attracted considerable attention in the field of drug delivery. The drug carrier system, which was revolutionized by the introduction of these polymers, has recently provided a new paradigm of maximizing the therapeutic activity of drugs. This review highlights recent studies regarding stimuli-responsive drug carriers tailor-made for effective cytosolic drug delivery, with particular emphasis on tumor treatment.

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A Protein Nanocarrier from Charge-Conversion Polymer in Response to Endosomal pH

Cited by 378 publications

References 13 publications

A functional nanocarrier that copenetrates extracellular matrix and multiple layers of tumor cells for sequential and deep tumor autophagy inhibitor and chemotherapeutic delivery

A functional nanocarrier that copenetrates extracellular matrix and multiple layers of tumor cells for sequential and deep tumor autophagy inhibitor and chemotherapeutic delivery

Multifunctional nanoassemblies of block copolymers for future cancer therapy

Intelligent Polymeric Nanocarriers Responding to Physical or Biological Signals: A New Paradigm of Cytosolic Drug Delivery for Tumor Treatment

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