A Protein‐Interaction Array Inside a Living Cell

Gandor, Silke; Venkatachalapathy, Muthukumaran; Arrabito, Giuseppe; Reibner, Martina; Schröder, Hendrik; Ruf, Katharina; Niemeyer, Christof M.; Bastiaens, Philippe I. H.; Dehmelt, Leif

doi:10.1002/ange.201209127

Cited by 17 publications

(14 citation statements)

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“…[63] DNA-based ligand arrays with subcellular dimensions (feature size ca. [64,65,67] Forexample, the ligand patterns can be used to specifically bind epitopes of transmembrane receptors in the plasma membrane of adhered cells,thus leading to recruitment and enrichment of the receptors on micropatterned substrates ( Figure 4B). [64,65,67] Forexample, the ligand patterns can be used to specifically bind epitopes of transmembrane receptors in the plasma membrane of adhered cells,thus leading to recruitment and enrichment of the receptors on micropatterned substrates ( Figure 4B).…”

Section: Dna Chips For Cell Cultivationmentioning

confidence: 99%

“…5 mm) can be used for functional manipulation of small regions inside living cells. The approach can be used for the analysis of native transmembrane receptor signaling, [64] the generation of protein-interaction arrays inside living cells that express synthetic baitpresenting artificial receptor constructs (Bait-PARCs,F igure 4D), [65] or for the dynamic light-controlled assembly of supramolecular protein structures that regulate the direction of the movement of living cells. The approach can be used for the analysis of native transmembrane receptor signaling, [64] the generation of protein-interaction arrays inside living cells that express synthetic baitpresenting artificial receptor constructs (Bait-PARCs,F igure 4D), [65] or for the dynamic light-controlled assembly of supramolecular protein structures that regulate the direction of the movement of living cells.…”

Section: Dna Chips For Cell Cultivationmentioning

confidence: 99%

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DNA Surface Technology: From Gene Sensors to Integrated Systems for Life and Materials Sciences

Schneider

Niemeyer

2018

Angewandte Chemie

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The evolution of DNA microarray technology has led to sophisticated DNA chips that are being used as routine tools for fundamental and applied genome research such as genotyping and expression profiling. Owing to their capability for highly parallel, site‐directed immobilization of complementary nucleic acids through canonical Watson–Crick base‐pairing, however, DNA‐modified surfaces can also be used for the assembly of complex surface architectures comprised of non‐nucleic acid compounds, such as proteins or colloidal materials. Furthermore, implementation of functional DNA devices and structural DNA nanotechnology can unlock the full potential of DNA surfaces. Based on case studies from diagnostics, sensing, proteome research, cell adhesion, and cell signaling, we show how classical gene sensors have developed into modern integrated systems and platforms for various applications in life sciences and materials research.

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Section: Dna Chips For Cell Cultivationmentioning

confidence: 99%

Section: Dna Chips For Cell Cultivationmentioning

confidence: 99%

DNA Surface Technology: From Gene Sensors to Integrated Systems for Life and Materials Sciences

Schneider

Niemeyer

2018

Angewandte Chemie

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“…A). This technique has allowed researchers to study cell adhesion and phagocytosis , signaling , cellular protein–protein interactions , and protein–ligand interactions . The advantage of using printed protein patterns to measure protein interactions is that the cell surface protein is in its native environment and composition, and that the assay can be performed in the living cell.…”

Section: Introduction: the Production And Use Of Protein Patterns So Farmentioning

confidence: 99%

Protein micropatterns printed on glass: Novel tools for protein‐ligand binding assays in live cells

Dirscherl

Springer

2017

Engineering in Life Sciences

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Micrometer‐sized patterns of proteins on glass or silica surfaces are in widespread use as protein arrays for probing with ligands or recombinant proteins. More recently, they have been used to capture the surface proteins of mammalian cells seeded onto them, and to arrange these surface proteins into pattern structures. Binding of small molecule ligands or of other proteins, transmembrane or intracellular, to these captured surface proteins can then be quantified. However, reproducible production of protein micropatterns on surfaces can be technically difficult. In this review, we outline the wide potential and the current practical uses of printed protein micropatterns in a historical overview, and we detail some potential pitfalls and difficulties from our own experience, as well as ways to circumvent them.

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“…The functionality of such arrays demonstrates the relevance of DPN as a method of choice for conducting studies inthe biological sciences. Forexample,Niemeyer and co-workersshowedthatDPN can be used to generate protein arrays and probe protein–receptor interactions inside living cells and that this method can be coupled with traditional biological techniques for the study of biological processes (Gandor et al, 2013). …”

Section: Introductionmentioning

confidence: 99%

Combinatorial Screening of Mesenchymal Stem Cell Adhesion and Differentiation Using Polymer Pen Lithography

Cabezas¹,

Eichelsdoerfer²,

Brown³

et al. 2014

Methods in Cell Biology

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The extracellular matrix (ECM) is a complex, spatially inhomogeneous environment that is host to myriad cell–receptor interactions that promote changes in cell behavior. These biological systems can be probed and simulated with engineered surfaces,but doing so demands careful control over the arrangement of ligands. Here, we describe how such surfaces can be fabricated by utilizing polymer pen lithography (PPL), which is a cantilever-free scanning probe lithographic method that utilizes polymeric pen arrays to generate patterns over large areas. With the advent of PPL, fundamental questions in cell biology can be answered by recapitulating cell–ECM interactions to explore how these interactions lead to changes in cell behavior. Here, we describe an approach for the combinatorial screening of cell adhesion behavior to gain understanding of how ECM protein feature size dictates osteogenic differentiation of mesenchymal stem cells. The technique outlined here is generalizable to other biological systems and can be paired with quantitative analytical methods to probe important processes such as cell polarization, proliferation, signaling, and differentiation.

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A Protein‐Interaction Array Inside a Living Cell

Cited by 17 publications

References 13 publications

DNA Surface Technology: From Gene Sensors to Integrated Systems for Life and Materials Sciences

DNA Surface Technology: From Gene Sensors to Integrated Systems for Life and Materials Sciences

Protein micropatterns printed on glass: Novel tools for protein‐ligand binding assays in live cells

Combinatorial Screening of Mesenchymal Stem Cell Adhesion and Differentiation Using Polymer Pen Lithography

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