A protein containing the DUF1471 domain regulates biofilm formation and capsule production in Klebsiella pneumoniae

Horng, Yu-Tze; Panjaitan, Novaria Sari Dewi; Chang, Hui-Ju; Wei, Yuhong; Chien, Chih-Ching; Yang, Hung‐Chi; Chang, Heng-Yuan; Soo, Po-Chi

doi:10.1016/j.jmii.2021.11.005

Cited by 9 publications

(6 citation statements)

References 28 publications

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“…However, in these conditions, type 3 fimbriae are highly expressed, suggesting a positive interaction between the capsule and this fimbriae during biofilm formation. Similarly, a recent report showed that, in conditions in which type 3 fimbriae is expressed, biofilm formation increases when capsule production is enhanced [46]. Indeed, in K. variicola, the mutations in MrkD only significantly increase biofilm in capsulated strains, but not in non-capsulated strains.…”

Section: Discussionmentioning

confidence: 79%

Latent evolution of biofilm formation depends on life-history and genetic background

Nucci

Rocha

Rendueles

2023

Preprint

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Adaptation to one environment can often generate phenotypic and genotypic changes which impact the future ability of an organism to thrive in other environmental conditions. In the context of host-microbe interactions, biofilm formation can increase survival rates in vivo upon exposure to stresses, like the immune system of the host or antibiotic therapy. However, how the generic process of adaptation impacts the ability to form biofilm and how it may change through time has seldomly been studied. To do so, we used a previous evolution experiment with three strains of the Klebsiella pneumoniae species complex, in which we did not specifically select for biofilm formation. We observed that changes in the ability to form biofilm happened very fast at first and afterwards reverted to ancestral levels in many populations. Biofilm changes were associated to phenotypic changes in population yield and surface polysaccharide production. Genotypically, mutational targets in the tip adhesin of type III fimbriae (mrkD) or the fim switch of type I fimbriae were driven by nutrient availability during evolution, and their impact on biofilm formation was dependent on capsule production. Analyses of natural isolates revealed similar mutations in mrkD, suggesting that they also play an important role in adaptation outside the laboratory. Our work reveals that the latent evolution of biofilm formation, and its evolutionary dynamics, depend on nutrient availability, the genetic background and other intertwined phenotypic and genotypic changes. Ultimately, it suggests that small differences in the environment can alter the fate of an organism in more complex niches like the host.

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Section: Discussionmentioning

confidence: 79%

Latent evolution of biofilm formation depends on life-history and genetic background

Nucci

Rocha

Rendueles

2023

Preprint

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“… 45 Peg.1292 was reported to decrease Kp adhesive capacity by negatively regulating type III fimbriae 46 but also to reduce capsule and biofilm formation. 47 Peg.4155 encodes Yibj, which can promote biofilm formation and motility. 48 Finally, peg.5364 encodes the YjfY protein, which has not been characterized.…”

Section: Resultsmentioning

confidence: 99%

Intestinal carbapenem-resistant Klebsiella pneumoniae undergoes complex transcriptional reprogramming following immune activation

David,

Czauderna,

Cheng

et al. 2024

Gut Microbes

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Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) is a significant threat to public health worldwide. The primary reservoir for CR-Kp is the intestinal tract. There, the bacterium is usually present at low density but can bloom following antibiotic treatment, mostly in hospital settings. The impact of disturbances in the intestinal environment on the fitness, survival, expansion, and drug susceptibility of this pathogen is not well-understood, yet it may be relevant to devise strategies to tackle CR-Kp colonization and infection. Here, we adopted an in vivo model to examine the transcriptional adaptation of a CR-Kp clinical isolate to immune activation in the intestine. We report that as early as 6 hours following host treatment with anti-CD3 antibody, CR-Kp underwent rapid transcriptional changes including downregulation of genes involved in sugar utilization and amino acid biosynthesis and upregulation of genes involved in amino acid uptake and catabolism, antibiotic resistance, and stress response. In agreement with these findings, treatment increased the concentration of oxidative species and amino acids in the mouse intestine. Genes encoding for proteins containing the domain of unknown function (DUF) 1471 were strongly upregulated, however their deletion did not impair CR-Kp fitness i n vivo upon immune activation. Transcription factor enrichment analysis identified the global regulator cAMP-Receptor Protein, CRP, as a potential orchestrator of the observed transcriptional signature. In keeping with the recognized role of CRP in regulating utilization of alternative carbon sources, crp deletion in CR-Kp resulted in strongly impaired gut colonization, although this effect was not amplified by immune activation. Thus, following intestinal colonization, which occurs in a CRP-dependent manner, CR-Kp can rapidly respond to immune cues by implementing a well-defined and complex transcriptional program whose direct relevance toward bacterial fitness warrants further investigation. Additional analyses utilizing this model may identify key factors to tackle CR-Kp colonization of the intestine.

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“…The comparative genomics and genome sequencing applications revealed 134 distinct capsule synthesis loci, also known as K-loci, in K. pneumoniae. Notably, strains with defects in the capsule architecture have been shown to display impaired biofilm formation . Encapsulation has a significant impact on biofilm formation and the survival of bacteria under harsh environmental conditions.…”

Section: Biofilms: Fundamental Perspectivementioning

confidence: 99%

“…( 70 ) Notably, strains with defects in the capsule architecture have been shown to display impaired biofilm formation. 71 Encapsulation has a significant impact on biofilm formation and the survival of bacteria under harsh environmental conditions. A recent study showed that without the capsule bacteria formed stronger biofilms, which may be due to an increase in the hydrophobic nature of their surfaces.…”

Section: Biofilms: Fundamental Perspectivementioning

confidence: 99%

Advances in Nanotechnology for Biofilm Inhibition

et al. 2023

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Biofilm-associated infections have emerged as a significant public health challenge due to their persistent nature and increased resistance to conventional treatment methods. The indiscriminate usage of antibiotics has made us susceptible to a range of multidrug-resistant pathogens. These pathogens show reduced susceptibility to antibiotics and increased intracellular survival. However, current methods for treating biofilms, such as smart materials and targeted drug delivery systems, have not been found effective in preventing biofilm formation. To address this challenge, nanotechnology has provided innovative solutions for preventing and treating biofilm formation by clinically relevant pathogens. Recent advances in nanotechnological strategies, including metallic nanoparticles, functionalized metallic nanoparticles, dendrimers, polymeric nanoparticles, cyclodextrin-based delivery, solid lipid nanoparticles, polymer drug conjugates, and liposomes, may provide valuable technological solutions against infectious diseases. Therefore, it is imperative to conduct a comprehensive review to summarize the recent advancements and limitations of advanced nanotechnologies. The present Review encompasses a summary of infectious agents, the mechanisms that lead to biofilm formation, and the impact of pathogens on human health. In a nutshell, this Review offers a comprehensive survey of the advanced nanotechnological solutions for managing infections. A detailed presentation has been made as to how these strategies may improve biofilm control and prevent infections. The key objective of this Review is to summarize the mechanisms, applications, and prospects of advanced nanotechnologies to provide a better understanding of their impact on biofilm formation by clinically relevant pathogens.

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A protein containing the DUF1471 domain regulates biofilm formation and capsule production in Klebsiella pneumoniae

Cited by 9 publications

References 28 publications

Latent evolution of biofilm formation depends on life-history and genetic background

Latent evolution of biofilm formation depends on life-history and genetic background

Intestinal carbapenem-resistant Klebsiella pneumoniae undergoes complex transcriptional reprogramming following immune activation

Advances in Nanotechnology for Biofilm Inhibition

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