A protective effect of calcium carbonate against arsenic toxicity on the Nile catfish, larias gariepinus

Abstract

The present study was intended to test the protective upshot of calcium carbonate against the gifted toxicity of arsenic to the Nile cat-fish (Clarias gariepinus). Enhanced hepatosomatic index (HSI) and reduced gonadosomatic index (GSI) and intestinal index (ISI) as well as some of the tested blood parameters were recorded for fishes down to arsenic spotlight. The plasma levels of aspartate aminotransferase (AST, EC 2.6.1.1), alanine aminotransferase (ALT, EC 2.6.1.2), total bilirubin, direct bilirubin, total lipids, glucose and total protein were significantly increased in fishes exposed to arsenic. Likewise, the activities of AST, ALT and lactate dehydrogenase (LDH, EC 1.1.1.27), in the liver and muscle were radically increased, whereas the total protein and glycogen contents in these organs were significantly abridged following arsenic exposure, this may be an indication of energy expenditure attributable to arsenic toxicity. The histological examinations of the liver and gills renowned arsenic induced degenerative changes of these organs. Furthermore, the addition of calcium carbonate as a liming agent induces reversibility of most of these arsenic-induced changes, especially those of fishes subjected to 1/20 LC 50 of arsenic. Consequently, calcium carbonate could be feasible to be used for the fortification of C. gariepinus in opposition to arsenic toxicity.

Arsenic trioxide (As2O3) induces apoptosis and necrosis mediated cell death through mitochondrial membrane potential damage and elevated production of reactive oxygen species in PLHC-1 fish cell line

“…The range of arsenic concentrations inducing effects in the PHLC1 cell line and the zebra fish liver cells and JP and T0-2 are similar to those found to induce cellular level changes in whole organisms (Abdel-Hameid, 2009). After 9 days of exposure to sub-lethal concentrations of arsenic, the nile catfish showed biochemical and histological changes.…”

Influence of cyanide on some antioxidant enzymes of freshwater fish, Cirrhinus mrigala (Hamilton)

“…Similar trends were also evidenced in liver (39.85% and 25.26%) and gill (19.13% and 8.09%) tissue of the fish exposed to cyanide (Table 2). Increased activity of LDH is a characteristic feature of a shift from aerobic to anaerobic metabolism leading to elevated rate of pyruvate conversion into lactate, resulting in lactic acidosis (Abdel-Hameid, 2009). Data from the present study demonstrated a significant increase in LDH activity under hypoxia.…”

Dietary Effects of Ripe and Unripe Banana Peels on The Growth and Economy Of Production Of Juvenile Catfish (Clarias gariepinus Burchell, 1822)

“…This may probably be a result of loss of appetite due to the presence of antinutritional factors in banana peels and concomitantly resulted in reduction of total body weight (Abdel-Hamed, 2009). This result is also in agreement with several other works where changes in intestinosomatic index values were used as indicator of physiological conditions and body weight loss caused by reduced feed intake (Abdel-Tawwab et al, 2006.…”