A proposed protocol for the standardized preparation of PRF membranes for clinical use

Kobayashi, Mito; Kawase, Tomoyuki; Horimizu, Makoto; Okuda, K; Wolff, Larry F.; Yoshie, Hiromasa

doi:10.1016/j.biologicals.2012.07.004

Cited by 107 publications

(120 citation statements)

References 17 publications

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“…Fibrin functions as an ''adhesive'' scaffolding material for adherent cells to concentrate at the site of tissue regeneration in vivo or to increase the number of cells in in vitro studies [22,23]. Additionally, fibrin functions as an ''adhesive'' carrier for growth factors to control their release and sustain their bioactivity for longer time periods [24,25].…”

Section: Major Factors In Prpmentioning

confidence: 99%

Platelet-rich plasma and its derivatives as promising bioactive materials for regenerative medicine: basic principles and concepts underlying recent advances

Kawase

2015

Odontology

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116

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Over the past decade, platelet-rich plasma (PRP), a platelet-concentrated plasma fraction, has been widely investigated and applied to regenerative medicine. The clinical utility of PRP is supported by evidence that PRP contains high concentrations of platelet-related growth factors and normal concentrations of plasma-derived fibrinogen, both of which contribute synergistically to the regenerative process. Additionally, its superior cost-efficacy versus conventional therapies is attractive to many clinicians. However, current disadvantages of PRP include a relatively complicated preparation procedure and variable operator-dependent efficacy. An additional disadvantage is the use of bovine thrombin, an animal-derived biological, as a coagulant. Many of these disadvantages are overcome by recent advances in preparation procedures and devices; for example, Joseph Choukroun simplified the platelet-rich fibrin preparation procedure and improved handling efficiency without the aid of animal-derived factors. With advancements in cell processing technology, there has been a general shift in cell therapy from autologous to allogeneic treatment; however, autologous PRP therapy will not easily be replaced by allogeneic treatment in the near future. Therefore, to provide more predictable regenerative therapy outcomes using autologous PRP, further investigations should address developing a standardized procedure for PRP preparation to augment its efficacy and potency, independent of donor variability. We would then propose that operators and clinicians prepare PRP according to the standardized protocol and to carefully evaluate the clinical scenario (i.e., recipient factors comprising skeletal defects) to determine which factor(s) should be added to PRP preparations. This careful approach will lead to improved clinical outcomes for patients.

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Section: Major Factors In Prpmentioning

confidence: 99%

Platelet-rich plasma and its derivatives as promising bioactive materials for regenerative medicine: basic principles and concepts underlying recent advances

Kawase

2015

Odontology

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116

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“…As described previously, 9 blood was collected from three healthy, nonsmoking volunteers aged 28-53 years (two females and one male) using Vacutainer TM tubes (Japan Becton, Dickinson and Company, Tokyo, Japan) and immediately centrifuged by a Medifuge centrifugation system (Silfradent S.r.l., Santa Sofia, Italy). After the red thrombus (fraction of red blood cells) was eliminated from the PRF preparations, the resulting PRF was rinsed with PBS and then compressed with dry gauze.…”

Section: Prf Preparationmentioning

confidence: 99%

The heat-compression technique for the conversion of platelet-rich fibrin preparation to a barrier membrane with a reduced rate of biodegradation

Kawase

Kamiya

Kobayashi

et al. 2014

J. Biomed. Mater. Res.

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Platelet-rich fibrin (PRF) was developed as an advanced form of platelet-rich plasma to eliminate xenofactors, such as bovine thrombin, and it is mainly used as a source of growth factor for tissue regeneration. Furthermore, although a minor application, PRF in a compressed membrane-like form has also been used as a substitute for commercially available barrier membranes in guided-tissue regeneration (GTR) treatment. However, the PRF membrane is resorbed within 2 weeks or less at implantation sites; therefore, it can barely maintain sufficient space for bone regeneration. In this study, we developed and optimized a heat-compression technique and tested the feasibility of the resulting PRF membrane. Freshly prepared human PRF was first compressed with dry gauze and subsequently with a hot iron. Biodegradability was microscopically examined in vitro by treatment with plasmin at 37°C or in vivo by subcutaneous implantation in nude mice. Compared with the control gauze-compressed PRF, the heat-compressed PRF appeared plasmin-resistant and remained stable for longer than 10 days in vitro. Additionally, in animal implantation studies, the heat-compressed PRF was observed at least for 3 weeks postimplantation in vivo whereas the control PRF was completely resorbed within 2 weeks. Therefore, these findings suggest that the heat-compression technique reduces the rate of biodegradation of the PRF membrane without sacrificing its biocompatibility and that the heat-compressed PRF membrane easily could be prepared at chair-side and applied as a barrier membrane in the GTR treatment.

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“…This is immediately followed by the centrifugation process, 3000rpm for 10 minutes. If not immediately centrifuged, diffuse polymerization of fibrin occurs, which results in a clot of reduced quantity and quality (Kobayashi et al 2012). After the processing of PRF, the sample of blood in the test tube is allowed to settle and in the process separates into three layers: The acellular plasma, or platelet-poor plasma (PPP) that is the topmost straw-colored layer, as the name suggests, is lacking in platelet cells.…”

Section: Preparation Of Prfmentioning

confidence: 99%

“…It is thought that this natural architecture is responsible for the slow release of growth factors. Such a a slow release has not been observed in PRP as it involves a very rapid and artificial platelet activation and hence has a very light and weak fibrin network (Kobayashi et al 2012, Simonpieri et al 2102). …”

Section: Architecture and Composition Of The Prf Membranementioning

confidence: 99%

Platelet rich fibrin in dentistry: A review of literature

Kumar

Gangadharan

2015

IJM

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<p>The aim of any invasive procedure is the complete eradication and elimination of the infection and associated necrotic tissue followed by repair and regeneration of the affected tissue. In order to achieve this goal various biological products have been introduced, among which are present the platelet concentrates. Platelet concentrates with their higher concentration of platelets has been used in the field of medicine since the 1990s in the form of the first-generation concentrates-Platelet Rich Plasma, and the second generation concentrated -Platelet Rich Fibrin. Choukroun’s Platelet Rich Fibrin is one such material, which is used by itself and also as an adjunct with grafts. It has been successful as it delivers high doses of growth factors and has anti-inflammatory properties. They have been shown to be of great promise in the field of dentistry, ranging from implantology; Sinuses lift procedures, treating of endodontic and periodontal lesions to regeneration of necrotic pulp. This novel technique has the potential to revolutionize the treatment mode in dentistry and help with reducing patient morbidity. We will be further looking into the specification and implications of this material in this review.</p>

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A proposed protocol for the standardized preparation of PRF membranes for clinical use

Cited by 107 publications

References 17 publications

Platelet-rich plasma and its derivatives as promising bioactive materials for regenerative medicine: basic principles and concepts underlying recent advances

Platelet-rich plasma and its derivatives as promising bioactive materials for regenerative medicine: basic principles and concepts underlying recent advances

The heat-compression technique for the conversion of platelet-rich fibrin preparation to a barrier membrane with a reduced rate of biodegradation

Platelet rich fibrin in dentistry: A review of literature

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