A proposed protocol based on integrative metabonomics analysis for the rapid detection and mechanistic understanding of sulfur fumigation of Chinese herbal medicines

Abstract

In the current work, Lonicera japonica Flos (FLJ) was selected as a model Chinese herbal medicine (CHM) and a protocol was proposed for the rapid detection of sulfur-fumigated (SF) CHMs.

A Practical Protocol for a Comprehensive Evaluation of Sulfur Fumigation of Trichosanthis Radix Based on Both Non-Targeted and Widely Targeted Metabolomics

“…Moreover, there are currently neither reports on the formation of new sulfur-fumigation markers during the sulfur fumigation process of TR nor reports on the regulation of the chemical conversion of different levels of sulfur fumigation. At present, non-targeted metabolomics and targeted metabolomics based on LC-QTOF-MS or LC-QTRAP-MS have become more mature in the analysis of the chemical composition of Chinese herbal medicines and the mining of quality control indicators ( Ma et al., 2014 ; Li et al., 2016 ; Dai et al., 2018 ; Wei et al., 2018 ; Zhang et al., 2018 ; Shengyun et al., 2019 ; Jiang et al., 2020 ). Specifically, broadly targeted metabolomics is a detection technology that integrates the “extensiveness” of non-targeted metabolomics with the “accuracy” of targeted metabolomics ( Luo et al., 2018 ; Zhu et al., 2018 ).…”