A Proposed Model of Boundary Lubrication by Synovial Fluid: Structuring of Boundary Water

Davis, W.; Lee, S. L.; Sokoloff, Leon

doi:10.1115/1.3426243

Cited by 58 publications

(31 citation statements)

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“…No explanation is offered among these reports as to why digestion of synovial fluid with hyaluronidase reduced viscosity to near that of water but did not affect lubrication adversely. 8,[22][23][24][25] In addition, proteolysis is well known to extinguish the lubricating ability of synovial fluid. Swann 9 showed no relationship between the concentration of hyaluronate and lubricating ability.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the boundary-lubricating ability of bovine synovial fluid, lubricin, and Healon

Jay

Haberstroh

Cha

1998

J. Biomed. Mater. Res.

130

107

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Purified human umbilical hyaluronate and a commercial preparation of rooster comb hyaluronate (Healon) intended for intra-articular viscosupplementation did not demonstrate the same degree of boundary-lubricating ability as bovine synovial fluid or its purified lubricating mucin, lubricin (p < 0.01). Boundary lubrication was measured in vitro in an arthrotripsometer oscillating natural latex against polished glass under a load of 0.35 MPa with an entraining velocity of 0.37 mm/s. The two hyaluronate solutions possessed the same hyaluronate concentration as synovial fluid, but Healon was 4.5 times more viscous. Present practice of viscosupplementation therapy for degenerative joint disease is limited and fails to implicate the important role of synovial mucin. Boundary lubrication provided by synovial mucin, independent of its viscosity, is not replicated by hyaluronate hydrogels.

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Section: Discussionmentioning

confidence: 99%

Comparison of the boundary-lubricating ability of bovine synovial fluid, lubricin, and Healon

Jay

Haberstroh

Cha

1998

J. Biomed. Mater. Res.

130

107

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“…Based on data described elsewhere (16) and discussed below, the surfaces actually employed in this study were a pure latex (condom) rubber attached peripherally to the upper test pieces by an O-ring and an optically polished lower glass ring having a contact surface area of 1.59 cm2. Measurements were made over a loading range of 4.35 to 46.6 pounds per square inch (PSI).…”

Section: Methodsmentioning

confidence: 99%

Boundary lubricating ability of synovial fluid in degenerative joint disease

Davis

Lee

Sokoloff

1978

Arthritis & Rheumatism

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The boundary lubricating ability of eleven synovial fluids was measured in a miniaturized latex-glass test system. The specimens were obtained at necropsy from knees in which the degree of degenerative joint disease varied from none to very severe. The lubricating ability of boundary lubricating ability under physiological conditions despite the fact that the test surfaces were not cartilaginous and the loading was relatively low (up to 47 pounds per square inch).the fluid was independent of the viscosity over a wide range of shear rates. It was not diminished even in advanced lesions. In two additional fluids, the mucin clot was poor; the lubricating ability of one of these was compromised. Thus, although degenerative joint disease, during its quiescent stages, is not associated with defective synovial lubrication, the possibility that transient defects might lead to cartilage wear during life has not been excluded. The measurements are believed to be valid indicators of Portions of this study were taken from a dissertation submitted by Warren H. Davis, Jr., in partial fulfillment of the requirements for the degree of Doctor of Philosophy from the State University of New York at Stony Brook.

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“…Lubricin lubricated the non-biologic test surfaces of latex and glass with the effectiveness of unfractionated synovial fluid [lo]. The nearly co-planar 1atex:glass bearing was loaded with 0.37 x 10' N/m' and oscillated at 0.37 mm/s [4,10]. These conditions favor boundary rather than hydrodynamic lubrication provided by viscosity but they are consistent with apposed cartilagecartilage surfaces which become deformed under physiologic pressures of 5 x lo6 N/m2 [15].…”

Section: Introductionmentioning

confidence: 99%

Homology of lubricin and superficial zone protein (SZP): Products of megakaryocyte stimulating factor (MSF) gene expression by human synovial fibroblasts and articular chondrocytes localized to chromosome 1q25

Jay

Tantravahi

Britt

et al. 2001

Journal Orthopaedic Research

229

180

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We have previously identified megdkaryocyte stimulating factor (MSF) gene expression by synovial fibroblasts as the origin of lubricin in the synovial cavity. Lubricin is a mucinous glycoprotein responsible for the boundary lubrication of articular cartilage. MSF has a significant homology to vitronectin and is composed of 12 exons. RNA was purified from human synovial fibroblasts and articular chondrocytes grown in vitro from tissue explants obtained from subjects without degenerative joint disease. RT-PCR was used with multiple complimentary primer pairs spanning the central mucin expressing exon 6 of the MSF gene and individual exons on both the N-and C-terminal sides of exon 6. Exons 2, 4 and 5 appear to be variably expressed by synovial fibroblasts and articular chondrocytes. Lubricating mucin, in the form of MSF, is expressed by both chondrocytes and synovial fibroblasts in vitro.Both lubricin and superficial zone protein (SZP), a related proteoglycan, share a similar primary structure but could differ in posttranslational modifications with 0-linked oligosaccharides which are predominant in lubricin and with limited amounts chondroitin and keratan sulfate found in SZP. Since most of the MSF exons are involved in the expression of lubricating mucin. a strong homology to vitronectin persists. It is therefore appropriate to consider that both SZP and lubricin occupy a new class of biomolecules termed tvihonectins. Screening of a human genome bacterial artificial chromsoine (BAC) library with a cDN A primer pair complimentary for exon 6 identified two clones. Both clones were complimentary for chromosome lq25 by in situ hybridization. This same locus was previously implicated in camptodactyl-arthropathy-pericarditis syndrome (CAP) by genetic mapping. It is hypothesized that CAP, a large joint arthropathy, may be associated with ineffective boundary lubrication provided by synovial fluid. 0

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A Proposed Model of Boundary Lubrication by Synovial Fluid: Structuring of Boundary Water

Cited by 58 publications

References 0 publications

Comparison of the boundary-lubricating ability of bovine synovial fluid, lubricin, and Healon

Comparison of the boundary-lubricating ability of bovine synovial fluid, lubricin, and Healon

Boundary lubricating ability of synovial fluid in degenerative joint disease

Homology of lubricin and superficial zone protein (SZP): Products of megakaryocyte stimulating factor (MSF) gene expression by human synovial fibroblasts and articular chondrocytes localized to chromosome 1q25

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