A proposed mechanism for the hydroxylation of carotenoids on Micro-Cel C

Ritacco, Robert P.; Britton, George; Simpson, Kenneth L.

doi:10.1021/jf00122a031

Cited by 1 publication

(1 citation statement)

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“…The chromatographic isolation and identification of carotenoids are well represented in the literature (Nells and De Leenheer, 1983;Ritacco et al, 1984; Stewart and Wheaton, 1973; Calabro et al, 1978;Hsieh and Karel, 1983;Noga and Lenz, 1983;Will and Ruddat, 1984;Kamikura, 1961;Bushway and Wilson, 1982; Stancher and Zonta, 1982;Tsukida et al, 1982 and references therein). However, these procedures are not well suited for routine analysis often due to lengthy sample preparation procedures and the use of solvent gradients.…”

Section: Introductionmentioning

confidence: 99%

Solid-phase extraction and HPLC determination of .beta.-cryptoxanthin and .alpha.- and .beta.-carotene in orange juice

Fisher¹,

Rouseff²

1986

J. Agric. Food Chem.

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A simplified method is presented to determine three important orange juice carotenoids. Nonaqueous reversed-phase (NARP) high-performance liquid chromatography (HPLC) employing acetonitrilemethylene chloride-methyl alcohol (65:25:10, v/v) and a C-18 column was used to separate /3-cryptoxanthin and a-and /3-carotene from other juice components. Detection was at 450 nm. A photodiode array detection (PDAD) system was used to obtain UV-visible spectra for chromatographic peak identification and to determine chromatographic peak purity. Methanol was used to extract the carotenoids from centrifuged juice solids. A C-18 solid-phase extraction column was used to clean up the saponified sample prior to HPLC analysis. Ratios of /3-carotene to -carotene and /3-cryptoxanthin to /3-carotene from repeated ( = 5) injections were determined with relative standard deviations of 3.3 and 2.6%, respectively. Average carotenoid ratios for Valencia orange juice were 1.08 and 2.31, respectively. Murcott juices had average carotenoid ratios of 18.1 and 5.8, respectively.

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Section: Introductionmentioning

confidence: 99%