A Proposed Antigenic Schemia for the Identification of Strains of Pseudomonas Aeruginosa

The Specificity of Agglutination Reactions of Pseudomonas Aeruginosa With O Antisera

“…A number of serotyping schemes have been proposed (Habs, 1957;Verder and Evans, 1961 ;Meitert, 1964;Lanyi, 1966-67;Homma et al, 1970) and several numbering systems have been employed for the 0 serotypes or serogroups of P. aeruginosa;we accept the proposal (P. V. Liu, personal communication, 1976) that the numbering system of Habs (1957) should be used for the first 12 serogroups and that higher numbers should be used for additional groups described subsequently by other workers (see also Bergan, 1975).Most of the 0 serogroups of P. aeruginosa are antigenically distinct, and strains can be allocated to them by agglutination tests with rabbit antisera, usually without need to remove cross-reacting antibodies by absorption. However, a minority of strains that are stable in saline suspension are agglutinated by several antisera prepared against apparently unrelated 0 serogroups.…”

“…A number of serotyping schemes have been proposed (Habs, 1957;Verder and Evans, 1961 ;Meitert, 1964;Lanyi, 1966-67;Homma et al, 1970) and several numbering systems have been employed for the 0 serotypes or serogroups of P. aeruginosa;…”

The Antibody Response to the Flagella of Pseudomonas Aeruginosa

“…Some investigators (Christie, 1948 ;Matsui, 1959 ;Verder and Evans, 1961) have recognised several heat-labile antigens, but others have failed to differentiate between 0 and H agglutination or did not report such findings in their publications. The present investigation has attempted to distinguish the flagellar antigens from other heat-labile antigens and to study the specific antibodies formed to flagella.…”

“…PLATE VI THE heat-stable somatic antigens of Pseudomonas aeruginosa-the cell-wall lipopolysaccharides-provide the basis of the most widely used methods for the serological typing of this organism (Habs, 1957;Verder and Evans, 1961 ;Liinyi, 1966-67;and Homma et al, 1970). These procedures, in which sera are prepared against boiled or autoclaved organisms, have the disadvantage that some of the groups or " types " defined are rather common and may constitute up to 10-20% of all strains encountered.…”

“…Sera prepared in animals against unheated organisms also contain antibodies against various inadequately defined heat-labile bacterial constituents, but attempts to make use of these for the construction of a serological typing system have given confusing results (Aoki, 1926;Munoz, Scherago and Weaver, 1945 ;Gaby, 1946 ;Christie, 1948 ;Mayr-Harting, 1948 ; van den Ende, 1952;Matsui, 1959;Verder and Evans, 1961;and Liinyi 1970). It seems that an essential preliminary to developing a useful typing system based on heat-labile antigens is to distinguish between the various classes of antigen, such as those of the flagella and the fimbriae (or pili; see, for example, Bradley, 1972), and possibly also of the bacterial body.…”