A prophenoloxidase from the Chinese mitten crab Eriocheir sinensis: Gene cloning, expression and activity analysis

Gai, Yunchao; Zhao, Jianmin; Song, Linsheng; Li, Chenghua; Zheng, Peilin; Qiu, Limei; Ni, Duojiao

doi:10.1016/j.fsi.2007.08.006

Cited by 96 publications

(46 citation statements)

References 52 publications

(64 reference statements)

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“…PO activity was determined using the method reported by Gai et al (2008), with a slight modifi cation. In brief, 50 μL serum was mixed with 150 μL 15 mmol/L dopamine dissolved in ddH 2 O and 5 μL SDS (1%).…”

Section: Enzyme Activity or Hemocyanin Content Determinationmentioning

confidence: 99%

Enhanced resistance of Portunus trituberculatus to Vibrio alginolyticus by selective breeding

Liu

Song

et al. 2012

Chin. J. Ocean. Limnol.

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We established a line (screened) of Portunus trituberculatus by selectively breeding individuals that survived from challenge with Vibrio alginolyticus , and compared the response of screened and unscreened (control) P. trituberculatus challenged with V. alginolyticus . We measured superoxide dismutase, catalase, acid phosphatase, alkaline phosphatase, and peroxidase activity and the content of hemocyanin in the plasma and phenoloxidase activity in serum. The cumulative survival rate after 24-h challenge with V . alginolyticus was signifi cantly higher in the screened crabs than in the unscreened crabs ( P <0.05). T-SOD and PO activity were signifi cantly lower in the screened stock than in the unscreened stock ( P <0.05). POD, CAT, and ACP activity and hemocyanin content were signifi cantly higher in the screened stock than in the unscreened stock. Our results suggest that the screened stock was more resistant to infection. Furthermore, the indices we measured may be used to evaluate the health state of P . trituberculatus .

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Section: Enzyme Activity or Hemocyanin Content Determinationmentioning

confidence: 99%

Enhanced resistance of Portunus trituberculatus to Vibrio alginolyticus by selective breeding

Liu

Song

et al. 2012

Chin. J. Ocean. Limnol.

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“…Chinese mitten crab (Eriocheir sinensis), an important commercial freshwater species of aquaculture, has been developed rapidly in the farming scale over the past few decades in China (Gai et al, 2008). Owing to intensive culture and environmental deterioration, however, it frequently suffers from serious infectious diseases caused by bacteria, viruses, parasites, rickettsia-like organisms, and other pathogens, which reduced their production and resulted in economic loss (Lv et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Effect of lipopolysaccharide on the hemocyte apoptosis of Eriocheir sinensis

Lyu

et al. 2015

J. Zhejiang Univ. Sci. B

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Abstract:In the present study, we investigated the possible toxicity mechanism of lipopolysaccharide (LPS) extracted from Gram-negative bacteria in Eriocheir sinensis hemocytes. Apoptotic hemocytes and reactive oxygen species (ROS) production induced by the LPS were monitored by the combination of flow cytometry and microscope observation. It was shown that LPS induced serious damage on the DNA and morphological changes in hemocytes, including cell shrinkage, fracture of nucleus membrane, margination, condensation and fragmentation of chromatin, and formation of apoptotic bodies indicating obvious hemocyte apoptosis. As compared with the control group, the apoptotic cell ratio increased to 30.61% and 39.01% after 1-h exposure and 57.72% and 75.01% after 2-h exposure to 1 and 10 μg/ml LPS, respectively (P<0.05). Significant outburst of ROS production was observed in LPS-treated hemocytes with approximately 176.6% of relative dichlorofluorescein mean fluorescence at 1-h exposure, followed by a drastic decline (P<0.05). These results indicated that LPS would induce oxidative stress on hemocytes from E. sinensis and cause ROS burst, DNA damage, and subsequently apoptosis. The process of ROS-mediated apoptosis might be one of the potential toxicity mechanisms of LPS on crustacean hemocytes.

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“…Enhanced phenoloxidase activity in probiotic treated groups clearly demonstrates shrimps' ability to respond to external challenges (Lee and Soderhall, 2002). The pro PO system plays a critical role in host defensive reactions, which could be activated by some microbial lipopolysaccharides and β-1,3-glucan to generate phenoloxidase (PO), an important enzyme in the process of melanisation (Gai et al, 2008;Figueroa-Pizano et al, 2014). The transcript levels of proPO generally corresponded to the activity monitored for the same enzyme (Gai et al, 2008).…”

Section: Evaluation Of Probiotics In Penaeus Vannameimentioning

confidence: 99%

“…The pro PO system plays a critical role in host defensive reactions, which could be activated by some microbial lipopolysaccharides and β-1,3-glucan to generate phenoloxidase (PO), an important enzyme in the process of melanisation (Gai et al, 2008;Figueroa-Pizano et al, 2014). The transcript levels of proPO generally corresponded to the activity monitored for the same enzyme (Gai et al, 2008). Furthermore, proPO activity was found to be significantly higher (p<0.05) in the S. cerevisiae probiotic fed (0.132±0.001U min -1 mg protein -1 ) group of P. vannamei and other probiotic groups (Bs = 0.130±0.002, Lr = 0.125±0.005, Lc = 0.124±0.005, Sb = 0.110±0.005, Ent.…”

Section: Evaluation Of Probiotics In Penaeus Vannameimentioning

confidence: 99%

Evaluation of different probiotic strains for growth performance and immunomodulation in Pacific white shrimp Penaeus vannamei Boone, 1931

et al. 2017

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The use of antibiotics in aquaculture can prevent certain diseases, however, its use is highly restricted due to several environmental and human health problems like development of antibacterial resistance. Probiotics are widely used for improving production of aquatic animals by means of improving water quality as well as by nutritional and immune modulation in animals thus, helping in preventing diseases. The present study was aimed to evaluate different strains of probiotics viz., a commercial probiotic, Bacillus subtilis, Enterococcus sp., Lactobacillus casei, Lactobacillus rhamnosus, Saccharomyces cerevisiae and Saccharomyces boulardii on the growth, microbial load and immunomodulatory performance of the Pacific white shrimp, Penaeus vannamei. Seven probiotic feeds each containing 5 x 10 9 CFU ml -1 of respective probiotics kg -1 of feed were prepared by top coating on a pellet feed containing 35% protein and duration of the experiment was 75 days. All of the 6 selected strains of probiotics except the commercial one influenced the growth significantly when fed to the shrimp, compared to control group. Specific growth rate (SGR), average daily growth (ADG) and survival rate were observed to be higher in probiotic fed groups. Maximum growth was recorded in S. cerevisiae fed groups (8.05±0.21 g), followed by B. subtilis (7.65±0.21 g), while the control animals showed an average growth of 4.85±0.49 g with percentage improvement in the range of 50-95% when compared to all other treatments. Total heterotrophic count significantly (p<0.05) increased in the rearing water of B. subtilis treated group (8.995± 0.021 x 10 3 cfu ml -1 ) when compared to that of control (5.475± 0.003 X 10 3 cfu ml -1 ) and total vibrio load was greatly reduced in B. subtilis (1.42 ± 0.04 x 10 3 cfu ml -1) and S. cerevisiae (1.47 ± 0.01 x 10 3 cfu ml -1 ) treated water compared to control (4.265 ± 0.06 x 10 3 cfu ml -1). Non-specific immunity in terms of total haemocyte count (THC) was found to be significantly (p<0.05) higher in B. subtilis (12.4±0.8 10 6 cells ml ) treated groups. The probiotic effect was found to be beneficial for better growth and immunomodulation, which was however found to be strain-specific.

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A prophenoloxidase from the Chinese mitten crab Eriocheir sinensis: Gene cloning, expression and activity analysis

Cited by 96 publications

References 52 publications

Enhanced resistance of Portunus trituberculatus to Vibrio alginolyticus by selective breeding

Enhanced resistance of Portunus trituberculatus to Vibrio alginolyticus by selective breeding

Effect of lipopolysaccharide on the hemocyte apoptosis of Eriocheir sinensis

Evaluation of different probiotic strains for growth performance and immunomodulation in Pacific white shrimp Penaeus vannamei Boone, 1931

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