A propeptide‐independent protease from <i>Tannerella</i> sp.6_1_58FAA_CT1 displays trypsin‐like specificity

Song, Qitao; Zhang, Xiaoyue; Li, Na; Shen, Jie; Cheng, Jiansong

doi:10.1002/jobm.201600486

Cited by 5 publications

(4 citation statements)

References 23 publications

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“…In summary we have furthered knowledge of this important genus of bacterium, prominent not only in the oral microbiome but also identified as part of the gut microbiome (Song et al 2017;Ardissone et al 2014;Renouf & Hendrich 2011) and expanded our overall knowledge of human-dwelling bacterial species. Figure S1.…”

Section: Discussionmentioning

confidence: 77%

Characterisation and pure culture of putative health-associated oral bacterium BU063 (Tannerella sp. HOT-286) reveals presence of a potentially novel glycosylated S-layer

Frey

Ansbro

Kamble

et al. 2018

FEMS Microbiology Letters

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Tannerella HOT-286 (phylotype BU063) is a recently identified novel filamentous Gram-negative anaerobic oral bacterium cultured for the first time recently in co-culture with Propionibacterium acnes. In contrast to the related periodontal disease-associated pathobiont Tannerella forsythia, it is considered a putative health-associated bacterium. In this paper, we identified that this organism could be grown in pure culture if N-acetyl muramic acid (NAM) was provided in the media, although surprisingly the genetic basis of this phenomenon is not likely to be due to a lack of NAM synthesis genes. During further microbiological investigations, we showed for the first time that T. HOT-286 possesses a prominent extracellular S-layer with a novel morphology putatively made up of two proteins modified with an unknown glycan. These data further our knowledge of this poorly understood organism and genus that is an important part of the oral and human microbiome.

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Section: Discussionmentioning

confidence: 77%

Characterisation and pure culture of putative health-associated oral bacterium BU063 (Tannerella sp. HOT-286) reveals presence of a potentially novel glycosylated S-layer

Frey

Ansbro

Kamble

et al. 2018

FEMS Microbiology Letters

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“…Various proteases have been implicated in T. forsythia's pathogenicity, including, e.g., PrtH proteases which are associated with attachment loss ( 26 ), a trypsin-like cysteine protease exhibiting both arginine- and citrulline-specific activities ( 27 ), and secretory KLIKK proteases capable of targeting diverse protein substrates such as collagen, gelatine, elastin and casein ( 28 ). It is noteworthy that transcripts of KLIKK proteases were detected in almost all gingival crevicular fluid samples where T. forsythia was present.…”

Section: Virulence Factors Of T Forsythiamentioning

confidence: 99%

The intriguing strategies of Tannerella forsythia's host interaction

Schäffer,

Andrukhov

2024

Front. Oral. Health

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Tannerella forsythia, a member of the “red complex” bacteria implicated in severe periodontitis, employs various survival strategies and virulence factors to interact with the host. It thrives as a late colonizer in the oral biofilm, relying on its unique adaptation mechanisms for persistence. Essential to its survival are the type 9 protein secretion system and O-glycosylation of proteins, crucial for host interaction and immune evasion. Virulence factors of T. forsythia, including sialidase and proteases, facilitate its pathogenicity by degrading host glycoproteins and proteins, respectively. Moreover, cell surface glycoproteins like the S-layer and BspA modulate host responses and bacterial adherence, influencing colonization and tissue invasion. Outer membrane vesicles and lipopolysaccharides further induce inflammatory responses, contributing to periodontal tissue destruction. Interactions with specific host cell types, including epithelial cells, polymorphonuclear leukocytes macrophages, and mesenchymal stromal cells, highlight the multifaceted nature of T. forsythia's pathogenicity. Notably, it can invade epithelial cells and impair PMN function, promoting dysregulated inflammation and bacterial survival. Comparative studies with periodontitis-associated Porphyromonas gingivalis reveal differences in protease activity and immune modulation, suggesting distinct roles in disease progression. T. forsythia's potential to influence oral antimicrobial defense through protease-mediated degradation and interactions with other bacteria underscores its significance in periodontal disease pathogenesis. However, understanding T. forsythia's precise role in host-microbiome interactions and its classification as a keystone pathogen requires further investigation. Challenges in translating research data stem from the complexity of the oral microbiome and biofilm dynamics, necessitating comprehensive studies to elucidate its clinical relevance and therapeutic implications in periodontitis management.

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“…Multiple research studies have successfully detected Tf in the subgingival biofilm of patients using various techniques [ 50 ]. Several potential virulence factors have been discovered in T. forsythia , including trypsin-like [ 191 ] and PrtH proteases [ 192 ], NanH [ 193 ], a leucine-rich repeat protein BspA [ 194 ], alpha-D-glucosidase, N-acetyl-beta-glucosaminidase [ 195 ], components of the bacterial S-layer, and methylglyoxal [ 196 ].…”

Section: Tannerella Forsythiamentioning

confidence: 99%

The Roles of Periodontal Bacteria in Atherosclerosis

Huang

Xie

et al. 2023

IJMS

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Atherosclerosis (AS) is an inflammatory vascular disease that constitutes a major underlying cause of cardiovascular diseases (CVD) and stroke. Infection is a contributing risk factor for AS. Epidemiological evidence has implicated individuals afflicted by periodontitis displaying an increased susceptibility to AS and CVD. This review concisely outlines several prevalent periodontal pathogens identified within atherosclerotic plaques, including Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Fusobacterium nucleatum. We review the existing epidemiological evidence elucidating the association between these pathogens and AS-related diseases, and the diverse mechanisms for which these pathogens may engage in AS, such as endothelial barrier disruption, immune system activation, facilitation of monocyte adhesion and aggregation, and promotion of foam cell formation, all of which contribute to the progression and destabilization of atherosclerotic plaques. Notably, the intricate interplay among bacteria underscores the complex impact of periodontitis on AS. In conclusion, advancing our understanding of the relationship between periodontal pathogens and AS will undoubtedly offer invaluable insights and potential therapeutic avenues for the prevention and management of AS.

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A propeptide‐independent protease from Tannerella sp.6_1_58FAA_CT1 displays trypsin‐like specificity

Cited by 5 publications

References 23 publications

Characterisation and pure culture of putative health-associated oral bacterium BU063 (Tannerella sp. HOT-286) reveals presence of a potentially novel glycosylated S-layer

Characterisation and pure culture of putative health-associated oral bacterium BU063 (Tannerella sp. HOT-286) reveals presence of a potentially novel glycosylated S-layer

The intriguing strategies of Tannerella forsythia's host interaction

The Roles of Periodontal Bacteria in Atherosclerosis

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