A proof-of-concept study utilising 2D NMR spectrometry for in situ characterisation and quantitation of key biomarkers and actives in tape stripped ex vivo human skin

Robertson, Cameron; Raj, Nidhin; Lucas, Robert A.; Coban, Tomris; Gresley, Adam Le

doi:10.1016/j.talanta.2021.122980

Cited by 1 publication

(1 citation statement)

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“…Abdominoplasty skin explants are increasingly used to make translational dermatological research discoveries in a broad range of disciplines including skin healing [ 11 ], compound testing [ 12 ], and the role of immune cells in disease [ 13 , 14 ]. However, there is growing evidence to suggest that the skin at different anatomical sites differs in multiple immunological contexts, including in immune cell prevalence, cytokine and chemokine production, and the production of anti-microbial peptides [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

Assessment of patient-to-patient and intra-individual human abdominal skin immune cell variability

et al. 2022

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IntroductionThe objective of the study was to characterize the baseline intra-individual and inter-individual variability of immune cell subsets within abdominoplasty skin specimens.Material and methodsAbdominoplasty biopsies were taken from 5 patients and analysed using the Vectra 3 automated quantitative pathology imaging system with inForm software.ResultsAdjacent skin regions demonstrated intra-patient variability in immune subset counts ranging from 1- to 5-fold. Inter-variability between patients was approximately 2- to 7-fold for most subsets, except for HLA-DR+ antigen presenting cells, which varied 19-fold.ConclusionsOur data highlight the importance of including multiple patients and multiple patient samples when designing dermatological studies that utilise abdominoplasty skin.

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