A promising crop for cadmium-contamination remediation: Broomcorn millet

Li, Jiajia; Zhang, Dazhong; Yuan, Yuhao; Chen, Pengliang; Zhang, Panpan; Jin, Fei; Yang, Qinghua; Feng, Baili

doi:10.1016/j.ecoenv.2021.112669

Cited by 9 publications

(6 citation statements)

References 40 publications

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“…Thus, it has been speculated that Cd is transported into cells via the transporters responsible for the transport of essential divalent cations [ 19 ]. Given our previous study showing that Cd and Zn concentrations are positively correlated in broomcorn millet under Cd stress [ 17 ], transporters associated with uptake and transport of Zn 2+ , such as ZIP family transporter, may play key roles in Cd uptake and transport in broomcorn millet. Based on transcriptome data, ZIP transporter family genes were induced by Cd stress and expressed differentially between the two broomcorn millet varieties, and these results were consistent with research on hemp [ 7 ], suggesting that ZIP transporters might regulate Cd uptake and influence Cd resistance.…”

Section: Discussionmentioning

confidence: 99%

“…Two broomcorn millet ( Panicum miliaceum L.) cultivars with contrasting Cd tolerances and translocation factors were used as materials. ‘Ningmi6’ is a Cd-sensitive variety, while ‘4452’ is a Cd-tolerant variety [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

“…Seeds of similar size and appearance were sterilized with 0.1% mercuric chloride (HgCl 2 ) for 10 min, rinsed thrice with sterile water, and placed on moistened filter paper in Petri dishes for 3 days at 25 °C in the dark. Seedling culture was carried out by referring to the method of Liu et al [ 17 ]. At the four-leaf stage, the seedlings were treated with nutrient solution containing 0 (control, CK), 5, 15, 30, or 60 μmol/L Cd (as CdCl 2 ·2.5H 2 O) with three replicates for each treatment.…”

Section: Methodsmentioning

confidence: 99%

“…The Cd concentration (CdC, mg·kg −1 dry weight) was determined using the method described by Liu et al [ 17 ]. The Cd certified reference material (GSB 04-1721-2004) was used as the standard for CdC determination.…”

Section: Methodsmentioning

confidence: 99%

“…Broomcorn millet has been referred to as both a “smart food” and “pioneer crop” because it exhibits high tolerance to various biotic and abiotic stresses and it is critical for enduring food security in the face of challenges such as the present COVID-19 pandemic [ 13 , 14 , 15 ]. Furthermore, a rich germplasm resource has been developed for broomcorn millet [ 16 ], and our previous research has revealed that some broomcorn millet varieties are hyperaccumulators with potential for Cd phytoextraction [ 17 ]. However, mechanisms of Cd tolerance, absorption, and transport in broomcorn millet are still unknown.…”

Section: Introductionmentioning

confidence: 99%

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Dynamic and Comparative Transcriptome Analyses Reveal Key Factors Contributing to Cadmium Tolerance in Broomcorn Millet

Li¹,

Zhang²,

Zhang³

et al. 2022

IJMS

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Broomcorn millet (Panicum miliaceum L.) has great potential in Cd phytoextraction, but its mechanisms are largely unknown. Two contrasting broomcorn millet varieties, ‘Ningmi6’ (Cd-sensitive variety) and ‘4452’ (Cd-tolerant variety), were investigated through morphological, physiological, and transcriptomic analyses to determine the factors responsible for their differential Cd tolerance and translocation. The Cd-tolerant variety can accumulate more Cd, and its cell wall and vacuole component Cd proportions were higher compared with the Cd-sensitive variety. Under Cd stress, the glutathione content and peroxidase activity of the Cd-tolerant variety were significantly higher than those of the Cd-sensitive variety. Additionally, weighted gene co-expression network analysis (WGCNA) revealed hub modules that were associated with Cd stress and/or variety. Notably, genes involved in these hub modules were significantly enriched for roles in glutathione metabolism, phenylpropanoid biosynthesis, ABC transport, and metal ion transport process. These results suggested that regulation of genes associated with cell wall precipitation and vacuole compartmentalization may increase Cd tolerance and reduce Cd translocation in the Cd-tolerant variety, although it can absorb more Cd. This study provides a foundation for exploring molecular mechanisms of Cd tolerance and transport in broomcorn millet and new insights into improving Cd phytoremediation with this crop through genetic engineering.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dynamic and Comparative Transcriptome Analyses Reveal Key Factors Contributing to Cadmium Tolerance in Broomcorn Millet

Li¹,

Zhang²,

Zhang³

et al. 2022

IJMS

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Comprehensive physiological, transcriptomic, and metabolomic analysis of the response of Panicum miliaceum L. roots to alkaline stress

Wang

et al. 2023

Land Degrad Dev

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Alkaline stress disrupts transcriptional expression and causes metabolite accumulation, thus affecting plant growth. However, there are gaps in the response mechanism of plants to alkaline stress at the molecular dimension. Broomcorn millet (Panicum miliaceum L.) is a pioneer plant for stress resistance also is a future smart food crop. To explore the physiological‐molecular response and adaptation mechanism of broomcorn millet root to alkaline stress, we conducted physiological, transcriptomic, and metabolomic analyses on roots of two broomcorn millet cultivars (alkaline‐sensitive S223 and alkaline‐tolerant T289) that were exposed to normal conditions (CK) and alkaline stress (AS) treatments (40 mM and a molar ratio of Na2CO3: NaHCO3 = 1:9) for 7 days. Alkaline stress inhibited mineral uptake in broomcorn millet roots and enhanced the antioxidant enzyme activities, malondialdehyde, and soluble substances, resulting in changes in growth, biomass, and root structure. Correspondingly, differentially expressed genes induced by alkaline stress were prominently enriched in the plant hormone signal transduction, mitogen‐activated protein kinase (MAPK) signaling, phenylpropanoid biosynthesis, ATP binding cassette (ABC) transporters, and other biological pathways. Similar results were obtained from the differentially accumulated metabolites analysis. In addition, joint transcriptome and metabolome analysis indicated that genes of phenylpropanoid and flavonoid biosynthesis pathways were upregulated or downregulated in AS groups, however, the related metabolite accumulations were inhibited. This study integrates multiple methods to uncover the mechanisms of broomcorn millet response to alkaline stress. These findings suggest a new direction for phytoremediation of alkaline lands.

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Integrating Genomics and Phenomics Tools to Dissect Climate Resilience Traits in Small Millets

Pramitha

Choudhary

Das

et al. 2022

Omics of Climate Resilient Small Millets

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A promising crop for cadmium-contamination remediation: Broomcorn millet

Cited by 9 publications

References 40 publications

Dynamic and Comparative Transcriptome Analyses Reveal Key Factors Contributing to Cadmium Tolerance in Broomcorn Millet

Dynamic and Comparative Transcriptome Analyses Reveal Key Factors Contributing to Cadmium Tolerance in Broomcorn Millet

Comprehensive physiological, transcriptomic, and metabolomic analysis of the response of Panicum miliaceum L. roots to alkaline stress

Integrating Genomics and Phenomics Tools to Dissect Climate Resilience Traits in Small Millets

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