A Promising Approach to Integrally Evaluate the Disease Outcome of Cerebral Ischemic Rats Based on Multiple-Biomarker Crosstalk

Abstract

Purpose The study was designed to evaluate the disease outcome based on multiple biomarkers related to cerebral ischemia. Methods Rats were randomly divided into sham, permanent middle cerebral artery occlusion, and edaravone-treated groups. Cerebral ischemia was induced by permanent middle cerebral artery occlusion surgery in rats. To form a simplified crosstalk network, the related multiple biomarkers were chosen as S100β, HIF-1α, IL-1β, PGI2, TXA2, and GSH-Px. The levels or activities of these biomarkers in plasma were detected before and after ischemia. Concurrently, neurological deficit scores and cerebral infarct volumes were assessed. Based on a mathematic model, network balance maps and three integral disruption parameters (k, φ, and u) of the simplified crosstalk network were achieved. Results The levels or activities of the related biomarkers and neurological deficit scores were significantly impacted by cerebral ischemia. The balance maps intuitively displayed the network disruption, and the integral disruption parameters quantitatively depicted the disruption state of the simplified network after cerebral ischemia. The integral disruption parameter u values correlated significantly with neurological deficit scores and infarct volumes. Conclusion Our results indicate that the approach based on crosstalk network may provide a new promising way to integrally evaluate the outcome of cerebral ischemia.

Specnuezhenide ameliorates ultraviolet‐induced skin photoaging in mice by regulating the Sirtuin 3/8‐Oxoguanine DNA glycosylase signal

“…Our study found that specnuezhenide, one of the main ingredients extracted from Ligustri Lucidi Fructus, ameliorates ultraviolet-induced skin aging in mice by decreasing epidermal thickness, MDA content, and SAβgal expression, and increasing the content of collagen. Consistent with that of mice given with the ROS scavenger edaravone, 23 specnuezhenide inhibits skin photoaging-related oxidative injury, inflammation, and dermal cell apoptosis. The results above suggest that specnuezhenide is a promising candidate on protect against skin photoaging.…”