A promising approach to an environmentally friendly pest management solution: nanocarrier-delivered dsRNA towards controlling the destructive invasive pest <i>Tuta absoluta</i>

Wang, Xiaodi; Ji, Shun-Xia; Bi, Siyan; Tang, Yanhong; Zhang, Guifen; Yan, Shuo; Wan, Fang‐Hao; Lv, Zhichuang; Liu, Wan‐Xue

doi:10.1039/d2en01076c

Cited by 10 publications

(8 citation statements)

References 84 publications

(101 reference statements)

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“…12,18,19 The tomato leaf miner is another disease-causing insect, which can be decreased using nanobioconjugates as a remedy for the ailment. 20 3.1. Types of Nanomaterials and Their Conjugates Used.…”

Section: Nanomaterial-based Rnai Delivery For Pest and Crop Managementmentioning

confidence: 99%

“…Per studies, it was observed that the nanocarrier mode of the RNAi gene dysregulating method to prevent pests and microbes like the African malaria mosquito, yellow fever mosquito, bean yellow mosaic virus, cucumber mosaic virus, Rhizoctonia solani , Fusarium oxysporum , Xanthomonas oryzae , and so on is a successful utilization. ,, The tomato leaf miner is another disease-causing insect, which can be decreased using nanobioconjugates as a remedy for the ailment …”

Section: Nanomaterial-based Rnai Delivery For Pest and Crop Managementmentioning

confidence: 99%

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A Combinatorial Nanobased Spray-Induced Gene Silencing Technique for Crop Protection and Improvement

Ghosh,

Patra,

Ray

2023

ACS Omega

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Recent research reports have shown that plant pests and pathogens have depleted the crop yield widely, which has led to an increased dependence on commercial pesticides and fungicides. Increased usage of these pesticides has also shown adverse effects on the environment, therefore many techniques have been implemented for solving the issue, some of which include using nanobioconjugates, RNA(i), which put into use double-stranded RNAs to inhibit gene expression. A more innovative and eco-friendly strategy includes spray induced gene silencing, which is being increasingly implemented. This review delves into the eco-friendly approach of spray induced gene silencing (SIGS) in combination with nanobioconjugates, which have been used concerning various plant hosts and their pathogens to provide improved protection. Furthermore, nanotechnological advancements have been understood by addressing the scientific gaps to provide a rationale for the development of updated techniques in crop protection.

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Section: Nanomaterial-based Rnai Delivery For Pest and Crop Managementmentioning

confidence: 99%

Section: Nanomaterial-based Rnai Delivery For Pest and Crop Managementmentioning

confidence: 99%

A Combinatorial Nanobased Spray-Induced Gene Silencing Technique for Crop Protection and Improvement

Ghosh,

Patra,

Ray

2023

ACS Omega

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“…Thabet et al found that using SiO 2 NPs was superior in preventing the spread of the cowpea aphid, Aphis craccivora . The tomato leaf miner, an invasive pest Tuta absoluta, could be controlled using dsRNA–NP complexes after specific target dsRNA delivery was successfully mediated by chitosan and star polycation nanomaterials …”

Section: Introductionmentioning

confidence: 99%

“…14 The tomato leaf miner, an invasive pest Tuta absoluta, could be controlled using dsRNA− NP complexes after specific target dsRNA delivery was successfully mediated by chitosan and star polycation nanomaterials. 15 Generally, secondary metabolites are triggered in plants in response to abiotic (drought, light, salt) and biotic (pathogens) stress. 16 Low doses of NPs are now explored as safer and more relevant to the environment, but they have yet to garner as much attention as their higher counterparts.…”

Section: Introductionmentioning

confidence: 99%

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Copper and Silver Nanoparticle Seed Priming and Foliar Spray Modulate Plant Growth and Thrips Infestation in Capsicum spp.

Mawale,

Nandini,

Giridhar

2024

ACS Omega

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Nanoparticles (NPs) have the potential to improve plant health and secondary metabolite production. In the present study, three different NPs, i.e., Ag, Cu, and Cu-Ag NPs were produced in the range from 25 to 86 nm, with zeta potentials ranging from −28.8 to −38.5 mV. The synthesized NPs were used for seed priming and foliar spray on three varieties of Capsicum annuum. L, i.e., Arka Sweta (AS), Arka Meghana (AM), and Arka Harita (AH) plants grown under greenhouse conditions. Seed priming at various concentrations of NPs (1, 10, 20 ppm) enhanced the seed germination (96%), seedling vigor index (2494−3112.66), seedling length (6−49%), and biomass (46%) of 45 days old Arka Meghana seedlings. Additionally, all plant tissues accumulated significantly higher amounts of chlorophyll (51−142%), carotenoids (23−94.2%), total phenolic content (73%), and total flavonoid content (57%), compared with the control (p ≤ 0.05). The foliar spray of NPs (20−100 ppm) has a protective effect on the chili plants against thrips infestation (30−76%). The foliar spray enhanced chlorophyll (15−62%), carotenoids (15−50%), total phenolic content (20−62%), total flavonoid content (64−99%), reducing sugars (15−97%), total antioxidant activity (15−142%), ferric reducing antioxidant power assay (15−109%), DPPH (129−54 mg mL −1 ), and capsaicinoids (capsaicin and dihydrocapsaicin) (82− 128%). This study illustrates that Ag, Cu, and Cu-Ag NPs suppress thrips infestation and proliferation with enhanced plant growth and biochemical activity, which is inversely proportional to the NP size. Chemical NPs play a crucial role in the economic significance of chili plants, offering a promising avenue for developing pesticides to effectively combat thrips infestation. This advancement holds great potential in enhancing the overall agronomic productivity of the chili crops.

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Trypsin‐encoding gene function of efficient star polycation nanomaterial‐mediated dsRNA feeding delivery system of Grapholita molesta

Lv,

Kassen,

Men

et al. 2024

Pest Management Science

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BACKGROUNDGrapholita molesta is an important and harmful fruit pest worldwide, with widespread feeding hosts. Trypsin, an indispensable hydrolytic digestive protease in the insect gut, is crucial in digestion, growth and development. We analyzed the characteristics of the trypsin‐encoding genes, screened for the optimal dose of RNAi mediated by nanocarriers, and investigated various indices of larval growth and development of G. molesta.RESULTSGut content (GC) and RNase A degraded double‐stranded RNA (dsRNA), with a faster degradation rate at higher concentrations. Star polycation (SPc) nanomaterials protected dsGFP from degradation by anion–cation binding and did not migrate through agarose gel. The key conserved motifs of the trypsin‐encoding genes were similar, exhibiting high homology with those in other lepidopteran insects. An interference efficiency of ≈70% was achieved with SPc nanomaterial‐mediated RNA interference with 0.05 μg dsRNA. The efficiency of continuous interference was stable. Trypsin activity, body weight of 8‐day‐old larvae, pupal weight and emergence rate were significantly reduced, and the larval stage was significantly prolonged.CONCLUSIONThe investigated trypsin gene is a key target gene in the growth and development of G. molesta. We investigated the efficiency and convenience of feeding SPc nanomaterials in a functional study of insects. Our results provide valuable data for the development of efficient trypsin‐targeting pesticides. © 2024 Society of Chemical Industry.

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A promising approach to an environmentally friendly pest management solution: nanocarrier-delivered dsRNA towards controlling the destructive invasive pest Tuta absoluta

Abstract: To develop an environmentally friendly pest controlling RNAi method for the destructive invasive pest Tuta absoluta, we analysed the binding ratio between the nanocarrier and dsRNA, performed morphological characterization, investigated...

Cited by 10 publications

References 84 publications

A Combinatorial Nanobased Spray-Induced Gene Silencing Technique for Crop Protection and Improvement

A Combinatorial Nanobased Spray-Induced Gene Silencing Technique for Crop Protection and Improvement

Copper and Silver Nanoparticle Seed Priming and Foliar Spray Modulate Plant Growth and Thrips Infestation in Capsicum spp.

Trypsin‐encoding gene function of efficient star polycation nanomaterial‐mediated dsRNA feeding delivery system of Grapholita molesta

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