A promiscuous mechanism to phase separate eukaryotic carbon fixation in the green lineage
James Barrett,
Mihris I. S. Naduthodi,
Yuwei Mao
et al.
Abstract:CO2 fixation is commonly limited by inefficiency of the CO2-fixing enzyme Rubisco. Eukaryotic algae concentrate and fix CO2 in phase-separated condensates called pyrenoids, which complete up to one-third of global CO2 fixation. Condensation of Rubisco in pyrenoids is dependent on interaction with disordered linker proteins that show little conservation between species. We developed a sequence-independent bioinformatic pipeline to identify linker proteins in green algae. We report the linker from Chlorella and … Show more
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