A programmed oxyradical burst causes hatching of mouse blastocysts

Thomas, M. Joy; Jain, Sudhir; Kumar, G. Pradeep; Laloraya, Malini

doi:10.1242/jcs.110.14.1597

Cited by 41 publications

(7 citation statements)

References 22 publications

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“…Several studies have highlighted the complex relationship between intracellular ROS levels and preimplantation embryo development. H 2 O 2 has been shown to increase during the first cell divisions, the 8-cell stage to increase blastulation capacity and hatching in mouse and bovine embryos [25,[53][54][55][56]. However, in CM of 7-8 cells embryos, no significant difference in MDA levels was found between day 1 and day 3.…”

Section: Discussionmentioning

confidence: 96%

Lipid Peroxidation Status in Embryo Culture Media: The Impact on Fertilization and Embryo Quality during IVF Cycles

Jamil¹,

Debbarh²,

Kabit³

et al. 2022

ARRB

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Background: The process of preimplantation embryo development in vitro represents a key phase during in vitro fertilization (IVF) cycles. It involves several regulatory signaling pathways as well as an optimized in vitro culture system. The resulting embryo quality helps to determine embryo competence before implantation and pregnancy outcomes. Reactive oxygen species (ROS) are known to play a major role in influencing the process of embryo development. Their role can be reflected in the regulation of signaling pathways as second messengers or in the irreversible cell alterations due to oxidative stress following an excess of ROS levels. Methods: In this study, we investigated the association between morphological embryo quality (fertilization, cleavage quality, and fragmentation levels) and lipid peroxidation levels (Malondialdehyde) in embryo culture media. After intracytoplasmic sperm injection (ICSI), a total of 103 oocytes were evaluated on day 1 and day 3 of their development, and their corresponding culture media were analyzed by estimating MDA levels using thiobarbituric acid. Results: The results showed no significant association between MDA levels in culture media and fertilization rate (p=0.3), sperm quality (p=0.99; p= 0.17; p=0.46; p=0.30; p=0.65; p=0.44; p=0.09; p=0.15; p=0.56), embryo fragmentation levels (p=0.79; p=0.40), AMH levels (p=0.31; p=0.36) and female age (p=0.60; p=0.34). However, we revealed a significant association between cleavage quality and MDA levels in the embryo environment (p=0.03). Conclusion: We conclude that oxidative stress in IVF culture media might be mainly associated with delayed embryonic development.

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Section: Discussionmentioning

confidence: 96%

Lipid Peroxidation Status in Embryo Culture Media: The Impact on Fertilization and Embryo Quality during IVF Cycles

Jamil¹,

Debbarh²,

Kabit³

et al. 2022

ARRB

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“…Peri-implantation blastocysts produce a burst of O 2 .− and decrease levels of SOD compared to pre-and post-implantation embryos [124]. In vitro, the addition of O 2 .− around the time of hatching results in thinning of the zona pellucida, suggesting that the burst of ROS production for in vivo blastocysts assists in hatching.…”

Section: The Preimplantation Embryomentioning

confidence: 98%

“…In vitro, the addition of O 2 .− around the time of hatching results in thinning of the zona pellucida, suggesting that the burst of ROS production for in vivo blastocysts assists in hatching. Furthermore, a range of O 2 • − scavengers used on both in vitro and in vivo blastocysts reduces hatching [124].…”

Section: The Preimplantation Embryomentioning

confidence: 99%

Redox Regulation and Oxidative Stress in Mammalian Oocytes and Embryos Developed In Vivo and In Vitro

Hardy

Day

Morris

2021

IJERPH

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Oocytes and preimplantation embryos require careful regulation of the redox environment for optimal development both in vivo and in vitro. Reactive oxygen species (ROS) are generated throughout development as a result of cellular metabolism and enzyme reactions. ROS production can result in (i) oxidative eustress, where ROS are helpful signalling molecules with beneficial physiological functions and where the redox state of the cell is maintained within homeostatic range by a closely coupled system of antioxidants and antioxidant enzymes, or (ii) oxidative distress, where excess ROS are deleterious and impair normal cellular function. in vitro culture of embryos exacerbates ROS production due to a range of issues including culture-medium composition and laboratory culture conditions. This increase in ROS can be detrimental not only to assisted reproductive success rates but can also result in epigenetic and genetic changes in the embryo, resulting in transgenerational effects. This review examines the effects of oxidative stress in the oocyte and preimplantation embryo in both the in vivo and in vitro environment, identifies mechanisms responsible for oxidative stress in the oocyte/embryo in culture and approaches to reduce these problems, and briefly examines the potential impacts on future generations.

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“…The escape of expanded blastocysts from the ZP (hatching) is an epilogue of embryonic development prior to implantation. Although several hypotheses have been proposed regarding the mechanism of hatching in mouse, bovine, equine, and human, 45 , 46 , 47 the actual process remains unknown. Two novel hatching patterns, termed “inward” and “outward,” were revealed by the hR‐TLC observation, using donated day‐2 frozen/thawed embryos, which were cultured up to the hatching stage.…”

Section: Main Findingsmentioning

confidence: 99%

Morphokinetic features in human embryos: Analysis by our original high‐resolution time‐lapse cinematography—Summary of the past two decades

Mio,

Yumoto,

Sugishima

et al. 2024

Reprod Medicine & Biology

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BackgroundThe pioneering work by Dr. Payne et al. in time‐lapse cinematography for observation of the morphokinetic features of human embryos inspired us to develop a new in vitro culture system with high‐resolution time‐lapse cinematography (hR‐TLC) back in 2001.MethodsThis in vitro culture system was capable of maintaining stable culture and was constructed on an inverted microscope stage. Embryos were observed and photographed noninvasively for an extended period, up to 7 days. The obtained images were displayed at a speed of 30 frames per second and individually analyzed.ResultsUsing hR‐TLC, human fertilization and subsequent embryonic development were visualized, revealing the time course of phenomena and many unusual dynamics.ConclusionIn this review, we summarize the results of our hR‐TLC analysis of early human embryonic development over the past 20 years. In the near future, it is expected that the vast amount of information obtained by hR‐TLC will be integrated into the AI system for further analysis and to provide feedback that will have the potential to improve clinical practice. In the era of SDGs and environmental awareness, we should be cautious about the direction in which AI can be utilized to avoid any further harm to the planet.

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A programmed oxyradical burst causes hatching of mouse blastocysts

Cited by 41 publications

References 22 publications

Lipid Peroxidation Status in Embryo Culture Media: The Impact on Fertilization and Embryo Quality during IVF Cycles

Lipid Peroxidation Status in Embryo Culture Media: The Impact on Fertilization and Embryo Quality during IVF Cycles

Redox Regulation and Oxidative Stress in Mammalian Oocytes and Embryos Developed In Vivo and In Vitro

Morphokinetic features in human embryos: Analysis by our original high‐resolution time‐lapse cinematography—Summary of the past two decades

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