A Progesterone-Induced Protein Increases the Synthesis of Asymmetric Antibodies

Kelemen, Krisztina; Bognar, Irene T.; Paál, M; Szekeres-Barthó, Júlia

doi:10.1006/cimm.1996.0016

Cited by 60 publications

(42 citation statements)

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“…It has been highly accepted that progesterone induces the production of Th2 cytokines in vivo and vitro (24,27,28). Szekeres-Bartho and colleagues and the Margni group (48,49) report that progesterone induces the secretion of PIBF through lymphocytes and may be involved in the synthesis of asymmetric Abs. PIBF has an antiabortive effect by suppressing NK activity and increases the secretion of Th2 cytokines by immunosuppressor cells (8,22,24).…”

Section: Cd8mentioning

confidence: 99%

Depletion of CD8+ Cells Abolishes the Pregnancy Protective Effect of Progesterone Substitution with Dydrogesterone in Mice by Altering the Th1/Th2 Cytokine Profile

Blois

Joachim

Kandil

et al. 2004

The Journal of Immunology

155

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One of the most remarkable immunological regulations is the maternal immune tolerance toward the fetal semiallograft during pregnancy, which has been referred to as immunity’s pregnant pause. Rejection of the semiallogeneic trophoblast cells must be selectively inhibited and pathways presumably include Th2 cytokines unopposed by Th1 cytokines. Steroid hormones, including progesterone, have similar effects. Low levels of progesterone and Th2 cytokines and high levels of Th1 cytokines are attributable for increased abortions in mammalians, which may be triggered by psychoemotional stress. Thus, the aim of the present study was to provide experimental evidence for the mechanism involved in the mediation of immune responses by endocrine signals during pregnancy and stress-triggered pregnancy failure. DBA/2J-mated CBA/J female mice were randomized in three groups: 1) control females, 2) mice exposed to stress on gestation day 5.5, and 3) mice exposed to stress and substituted with dydrogesterone, a progestogen with a binding profile highly selective for the progesterone receptor on gestation day 5.5. On gestation days 7.5, 9.5, and 10.5, mice of each group were sacrificed, and the frequency of CD8+ cells and cytokine expression (IL-4, IL-12, TNF-α, IFN-γ) in blood and uterus cells was evaluated by flow cytometry. Additionally, some mice were depleted of CD8 cells by injection of mAb. We observed that progesterone substitution abrogated the abortogenic effects of stress exposure by decreasing the frequency of abortogenic cytokines. This pathway was exceedingly CD8-dependent, because depletion of CD8 led to a termination of the pregnancy protective effect of progesterone substitution.

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Section: Cd8mentioning

confidence: 99%

Depletion of CD8+ Cells Abolishes the Pregnancy Protective Effect of Progesterone Substitution with Dydrogesterone in Mice by Altering the Th1/Th2 Cytokine Profile

Blois

Joachim

Kandil

et al. 2004

The Journal of Immunology

155

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“…Others have also found that multiple vaccination during pregnancy does not necessarily increase the concentration or avidity of antibodies [43,44]. Considering that hyperimmunization may produce adverse reactions [22,45], and also that placental factors may influence not only the level but also the quality of the antibodies produced [44,46], the potential risks of unnecessary vaccination during consecutive pregnancies should be evaluated. The current national vaccination schedule proved to be effective at conferring immunity against tetanus, since all children became fully protected after the second dose of DTP vaccine and high protective levels were maintained by booster doses.…”

Section: Discussionmentioning

confidence: 99%

Serum antibodies to diphtheria-tetanus-pertussis vaccine components in Argentine children

et al. 1997

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The Argentine vaccination schedule against diphtheria, tetanus and pertussis (DTP) recommends three doses of DTP vaccine at 2, 4 and 6 months of age, two boosters at 18 months and 6 years, a booster dose of tetanus vaccine every 10 years and two doses during pregnancy. To evaluate the effect of this schedule, antibodies against pertussis toxin (PT) and filamentous hemagglutinin (FHA) and against tetanus and diphtheria toxoids were determined by ELISA in serum samples from children (1 month to 6 years) who received different doses of DPT vaccine: 0 dose (n = 50), 1 dose (n = 25), 2 doses (n = 25), 3 doses (n = 55), first and second booster (n = 25); 25 pregnant women and their offspring, and 45 adults. High antibody levels against PT (> 140 EU/ml) and FHA (> 80 EU/ml) were recorded in mothers and in the newborn. Antibody titers against PT increased with the number of doses given and decreased with time. Full protection against tetanus (titers > 0.1 IU/ml) was observed in the group of adults (0.37 IU/ml), in mothers (4.4 IU/ml) and their newborn offspring (5.5 IU/ml), and in children after receiving the second dose of DTP vaccine (1.86 IU/ml). The immune status for diphtheria was far lower, as most of the groups lacked adequate protection. After the third dose of DTP vaccine, only 78% of the children had antibody titers above the protective level (0.1 IU/ml). Since antibody levels considered to provide full protection were only achieved after the first booster dose of DTP vaccine, the primary three-dose schedule seems to be insufficient to confer adequate immunity in all vaccinees. Because of the high proportion of non-protected adults, a booster dose of Td vaccine should be considered for this group.

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“…The percent of asymmetric IgG was significantly higher in supernatants of hybridoma cells cultured in the presence of PIBF than in those cultured in the absence of PIBF, and there was a positive relationship between asymmetric antibody content of pregnancy sera and PIBF expression on lymphocytes from the same women. Furthermore, blocking of progesterone receptors by RU 486 or neutralizing endogenous PIBF activity by specific anti-PIBF antibodies significantly reduced the production of asymmetric antibodies in pregnant mice [92]. The above-mentioned biological effects of PIBF suggest that it might contribute to the maintenance of a normal pregnancy.…”

Section: Progesterone-induced Blocking Factormentioning

confidence: 97%

“…Silencing of STAT6 by siRNA reduces the cytokine effects [90]. The effect of PIBF on humoral immune responses includes the induction of asymmetric antibody production [91,92]. This population of antibodies, owing to the presence of a mannoserich oligosaccharide residue on one of the Fab arms of the molecule, does not precipitate; however, these antibodies might have a blocking effect.…”

Section: Progesterone-induced Blocking Factormentioning

confidence: 99%

Reproductive Immunology – an Update

Poehlmann

Fitzgerald

Busch

et al. 2006

Transfus Med Hemother

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Pregnancy is one of the most challenging experiences for the immune system. It entails the confrontation and cooperation of maternal cells with allogeneic (sperm) and semi-allogeneic (fetal) cells and factors. On the one hand, it must actively acquire a specific tolerance towards the foreign cells and organism (the fetus) to avoid harming reactions, while recognizing the very same to support and control their development and growth. On the other hand, the immune system simultaneously may not reduce its capability to defend mother and fetus from microorganisms and pathogens. Almost all branches of the immune system are claimed to react and adapt in order to fulfill these complex duties. In this review, current knowledge concerning the most important cellular and soluble immunological components in the decidua is presented. Special regards are made to decidual NK, T and dendritic cells as well as to trophoblast cells, representing the fetal counterpart of most bilateral interactions. Furthermore, the role and functions of soluble factors, including HLA-G, PIBF, IDO and a variety of cytokines, are described.

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A Progesterone-Induced Protein Increases the Synthesis of Asymmetric Antibodies

Cited by 60 publications

References 0 publications

Depletion of CD8+ Cells Abolishes the Pregnancy Protective Effect of Progesterone Substitution with Dydrogesterone in Mice by Altering the Th1/Th2 Cytokine Profile

Depletion of CD8+ Cells Abolishes the Pregnancy Protective Effect of Progesterone Substitution with Dydrogesterone in Mice by Altering the Th1/Th2 Cytokine Profile

Serum antibodies to diphtheria-tetanus-pertussis vaccine components in Argentine children

Reproductive Immunology – an Update

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