A profile of the GenePOC Carba C assay for the detection and differentiation of gene sequences associated with carbapenem-non-susceptibility

Baeza, Luis Lucena; Hamprecht, Axel

doi:10.1080/14737159.2020.1785287

Cited by 3 publications

(1 citation statement)

References 95 publications

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“…The predecessor Check-MDR CT103 performed well in the study by Cuzon et al, with sensitivity and specificity of 95–100% and 100%, respectively [ 84 ]. Other tests for CPE detection from rectal swabs include the Xpert Carba-R assay for the GeneXpert system (Cepheid, Sunnyvale, California [ 139 , 140 , 141 ]), the CRE ELITe MGB Kit (ELITechGroup, Puteaux, France [ 85 ]), GenePOC/Revogene Carba C assay (Meridian Bioscience, Cincinnati, Ohio [ 46 ]), and assays developed by MOBIDIAG for the Amplidiag ® Easy system, namely, Amplidiag CarbaR + VRE and Amplidiag CarbaR-MCR assays (Mobidiag Ltd., Espoo, Finland [ 86 , 87 ]).…”

Section: Detection and Characterization Of Mdr Enterobacteralesmentioning

confidence: 99%

Detection of Multidrug-Resistant Enterobacterales—From ESBLs to Carbapenemases

2021

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Multidrug-resistant Enterobacterales (MDRE) are an emerging threat to global health, leading to rising health care costs, morbidity and mortality. Multidrug-resistance is commonly caused by different β-lactamases (e.g., ESBLs and carbapenemases), sometimes in combination with other resistance mechanisms (e.g., porin loss, efflux). The continuous spread of MDRE among patients in hospital settings and the healthy population require adjustments in healthcare management and routine diagnostics. Rapid and reliable detection of MDRE infections as well as gastrointestinal colonization is key to guide therapy and infection control measures. However, proper implementation of these strategies requires diagnostic methods with short time-to-result, high sensitivity and specificity. Therefore, research on new techniques and improvement of already established protocols is inevitable. In this review, current methods for detection of MDRE are summarized with focus on culture based and molecular techniques, which are useful for the clinical microbiology laboratory.

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Section: Detection and Characterization Of Mdr Enterobacteralesmentioning

confidence: 99%

Detection of Multidrug-Resistant Enterobacterales—From ESBLs to Carbapenemases

2021

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Recent advances in rapid antimicrobial susceptibility testing systems

Jacobs

Colson

Rhoads

2021

Expert Review of Molecular Diagnostics

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Genetic Diversity, Biochemical Properties, and Detection Methods of Minor Carbapenemases in Enterobacterales

et al. 2021

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Gram-negative bacteria, especially Enterobacterales, have emerged as major players in antimicrobial resistance worldwide. Resistance may affect all major classes of anti-gram-negative agents, becoming multidrug resistant or even pan-drug resistant. Currently, β-lactamase-mediated resistance does not spare even the most powerful β-lactams (carbapenems), whose activity is challenged by carbapenemases. The dissemination of carbapenemases-encoding genes among Enterobacterales is a matter of concern, given the importance of carbapenems to treat nosocomial infections. Based on their amino acid sequences, carbapenemases are grouped into three major classes. Classes A and D use an active-site serine to catalyze hydrolysis, while class B (MBLs) require one or two zinc ions for their activity. The most important and clinically relevant carbapenemases are KPC, IMP/VIM/NDM, and OXA-48. However, several carbapenemases belonging to the different classes are less frequently detected. They correspond to class A (SME-, Nmc-A/IMI-, SFC-, GES-, BIC-like…), to class B (GIM, TMB, LMB…), class C (CMY-10 and ACT-28), and to class D (OXA-372). This review will address the genetic diversity, biochemical properties, and detection methods of minor acquired carbapenemases in Enterobacterales.

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A profile of the GenePOC Carba C assay for the detection and differentiation of gene sequences associated with carbapenem-non-susceptibility

Cited by 3 publications

References 95 publications

Detection of Multidrug-Resistant Enterobacterales—From ESBLs to Carbapenemases

Detection of Multidrug-Resistant Enterobacterales—From ESBLs to Carbapenemases

Recent advances in rapid antimicrobial susceptibility testing systems

Genetic Diversity, Biochemical Properties, and Detection Methods of Minor Carbapenemases in Enterobacterales

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