A procedure for successful nonsurgical embryo transfer in swine

Galvin, J; Killian, D. B.; Stewart, Amy

doi:10.1016/0093-691x(94)90486-3

Cited by 35 publications

(31 citation statements)

References 19 publications

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“…Pregnancy rates reported in this study were be�er than or comparable to data reported by Reichenbach et al (1993), Galvin et al (1994), Modl (1994), Hazeleger et al (1995a,b), Wallenhorst and Holtz (1995), Gordon (1997), Cuello et al (2002), Fujino et al (2003). Similar results were also found in our previous studies (Říha et al, 1996, 1997aVejnar, 2000, 2003).…”

Section: Discussioncontrasting

confidence: 45%

“…Sims and First (1987) used a non-surgical method for ET in pigs without any success. Reichenbach et al (1993), Galvin et al (1994), Modl (1994), Hazeleger et al (1995a,b), Wallenhorst and Holz (1995) successfully used non-surgical methods for transcervical transfers of fresh porcine embryos using a catheter which was similar to the catheter used in our experiments. Li et al (1995) and Gordon (1997) mentioned the importance of catheter construction for the success of ET in pigs.…”

Section: Discussionmentioning

confidence: 99%

“…The anatomical structure of the cervix and the small orifice of the cervical channel complicate the penetration of a catheter into the uterus lumen. Despite some success in non-surgical methods of transcervical transfers of fresh porcine embryos (Reichenbach et al, 1993;Galvin et al, 1994;Modl, 1994;Hazeleger et al, 1995a,b;Wallenhorst and Holz, 1995) the methods for porcine ET are still in the experimental phase of their development.…”

Section: Original Papermentioning

confidence: 99%

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Comparison of two vitrification methods for cryopreservation of porcine embryos

Řı́ha¹,

Vejnar²

2004

CZECH J ANIM SCI

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The aim of this study was to compare two vitrification methods of porcine perihatching blastocysts with regard to the success of transfer of these embryos to the recipients. Expanded, hatching, or hatched blastocysts were recovered post mortem from superovulated donors in 5.5 to 6.0 days after artificial insemination of donor gilts with homospermic doses. In protocol VS I, the embryos in perihatching developmental stage were equilibrated in a culture medium H-MEMD with 10% v/v of glycerol (1.37M solution of glycerol in medium) for 10 min and placed in a vitrification medium for 1.5 min max. (vitrification medium contained 50% v/v 2M sucrose in tridistilled water, 30% v/v of glycerol, and 20% v/v of foetal calf serum – FCS). Then they were dropped with micropipette and stored in liquid nitrogen vapour. For protocol VS II, we used H-MEMD culture medium supplemented with 20% v/v of FCS, 25% v/v ethylene glycol, and 25% v/v dimethyl sulphoxide (DMSO). Embryos were equilibrated for 10 min in a mixture of the vitrification medium and culture medium (1 : 1), and were kept in the vitrification medium for 1.5 minutes. Then they were dropped with micropipette and stored in liquid nitrogen vapour. Embryos were thawed by immersing the drop with the embryo in H-MEMD culture medium with 0.8M sucrose for 10 minutes. After thawing and washing in the medium with sucrose, all embryos were washed three times in a fresh medium and prepared for transfer. Recipients were synchronized either using Regumate-feeding followed by treatment with PMSG and HCG (gilts) or using piglet weaning (sows – 1st and 2nd parity). Recipients showing standing heat at the time of donor insemination were used for laparoscopic and non-surgical ET on day 5.5–6.0 of the cycle. The fraction of viable embryo vitrified under VS I or VS II protocol was 85% and 80%, compared to 95% in control fresh embryos (P > 0.05). Pregnancy of recipients was 57.3% (5/7), 67.0% (4/6) for VS I or VS II group and 42.7% (10/23) for control (P < 0.001). We can conclude on the basis of our data that both protocols for vitrification yielded similar results and can be used for cryopreservation of porcine embryos.  

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Section: Discussioncontrasting

confidence: 45%

Section: Discussionmentioning

confidence: 99%

Section: Original Papermentioning

confidence: 99%

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Comparison of two vitrification methods for cryopreservation of porcine embryos

Řı́ha¹,

Vejnar²

2004

CZECH J ANIM SCI

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“…The present study was conducted to perform transcervical transfer of pig embryos under more practical conditions than those published previously [3,4], focusing on infusion with large volumes of transfer medium with the embryos, and using a spiral catheter as a transfer instrument without anesthetizing or tranquilizing recipients.…”

mentioning

confidence: 99%

Transcervical Transfer of Porcine Embryos under Practical Conditions.

Yonemura¹,

Fujino²,

Irie³

et al. 1996

Journal of Reproduction and Development

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“…Several attempts were done to transfer embryos non-surgically using AI-spirettes or special designed instruments [31,39,58]. Despite several limitations (i.e.…”

Section: Embryo Transfermentioning

confidence: 99%