A prion-like domain in ELF3 functions as a thermosensor in Arabidopsis

Jung, Jae‐Hoon; Barbosa, António Daniel; Hutin, Stephanie; Kumita, Janet R.; Gao, Ming‐Jun; Derwort, Dorothee; Silva, Catarina S.; Lai, Xuelei; Pierre, Elodie; Geng, Feng; Kim, Sol-Bi; Baek, Sujeong; Zubieta, Chloé; Jaeger, Katja E.; Wigge, Philip A.

doi:10.1038/s41586-020-2644-7

Cited by 366 publications

(452 citation statements)

References 24 publications

(8 reference statements)

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“…For instance, a shift to cold conditions (from 18 to 4 • C) induces a nearly threefold increase in the production of the zwitterion DMSP in U. mutabilis (Kessler et al, 2017) when compared with that produced by green macroalgae of the genera Ulothrix and Acrosiphonia (Karsten et al, 1992). Although thermosensors (such as the prion domain of EARLY FLOWERING 3) have been recently identified in the flowering plant Arabidopsis thaliana (Jung et al, 2020), the thermosensory mechanisms are still unknown in macroalgae.…”

Section: Cold Temperature Stress and Growth Of Bacteriamentioning

confidence: 99%

Microbiome-Dependent Adaptation of Seaweeds Under Environmental Stresses: A Perspective

2020

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The microbiome of macroalgae facilitates their adaptation to environmental stress. As bacteria release algal growth and morphogenesis promoting factors (AGMPFs), which are necessary for the healthy development of macroalgae, bacteria play a crucial role in stress adaptation of bacterial-algal interactions. To better understand the level of macroalgal dependence on the microbiome under various stress factors such as light, temperature, salt, or micropollutants, we propose a reductionist analysis of a tripartite model system consisting of the axenic green alga Ulva (Chlorophyta) re-infected with two essential bacteria. This analysis will allow us to decipher the stress response of each symbiont within this cross-kingdom interaction. The paper highlights studies on possible survival strategies embedded in cross-kingdom interactions that govern the stress adaptation, including general features of metabolic pathways in the macroalgal host or more specific features such as alterations in the composition and/or diversity of bacterial assemblages within the microbiome community. Additionally, we present some preliminary results regarding the effect of recently isolated bacteria from the Potter Cove, King George Island (Isla 25 de Mayo) in Antarctica, on the model system Ulva mutabilis Føyn purified gametes. The results indicate that cold-adapted bacteria release AGMPFs, inducing cell differentiation, and cell division in purified cultures. We propose that microbiome engineering can be used to increase the adaptability of macroalgae to stressful situations with implications for, e.g., the sustainable management of (land-based) aquaculture systems.

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Section: Cold Temperature Stress and Growth Of Bacteriamentioning

confidence: 99%

Microbiome-Dependent Adaptation of Seaweeds Under Environmental Stresses: A Perspective

2020

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“…The growth‐promoting increase in PIF activity during thermomorphogenesis results from a few different mechanisms (Figure 6). First of all, warm temperatures inhibit the activity of EC by reducing its interaction with target promoters (Mizuno et al ., 2014; Box et al ., 2015; Ezer et al ., 2017; Silva et al ., 2020), which is likely to be partially explained by the observation that ELF3 aggregates at higher temperatures due to the presence of a prion‐like domain in this protein (Jung et al ., 2020). In diurnal conditions, this leads to increased PIF4 expression and, consequently, higher transcription of growth‐promoting genes during the night (Nomoto et al ., 2012a; Nomoto et al ., 2013; Mizuno et al ., 2014; Box et al ., 2015; Raschke et al ., 2015).…”

Section: Promotion Of Hypocotyl and Petiole Growth By Thermomorphogenmentioning

confidence: 99%

Molecular pathways regulating elongation of aerial plant organs: a focus on light, the circadian clock, and temperature

2020

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Summary Organs such as hypocotyls and petioles rapidly elongate in response to shade and temperature cues, contributing to adaptive responses that improve plant fitness. Growth plasticity in these organs is achieved through a complex network of molecular signals. Besides conveying information from the environment, this signaling network also transduces internal signals, such as those associated with the circadian clock. A number of studies performed in Arabidopsis hypocotyls, and to a lesser degree in petioles, have been informative for understanding the signaling networks that regulate elongation of aerial plant organs. In particular, substantial progress has been made towards understanding the molecular mechanisms that regulate responses to light, the circadian clock, and temperature. Signals derived from these three stimuli converge on the BAP module, a set of three different types of transcription factors that interdependently promote gene transcription and growth. Additional key positive regulators of growth that are also affected by environmental cues include the CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) and SUPPRESSOR OF PHYA‐105 (SPA) E3 ubiquitin ligase proteins. In this review we summarize the key signaling pathways that regulate the growth of hypocotyls and petioles, focusing specifically on molecular mechanisms important for transducing signals derived from light, the circadian clock, and temperature. While it is clear that similarities abound between the signaling networks at play in these two organs, there are also important differences between the mechanisms regulating growth in hypocotyls and petioles.

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“…In the model plant Arabidopsis thaliana , the red-light photoreceptor phytochrome B (phyB) senses temperature by a process called thermal relaxation, where the active Pfr form of phyB is converted back to the inactive Pr form by high ambient temperature (Jung et al , 2016; Legris et al , 2016). A prion-like domain in EARLY FLOWERING 3 (ELF3) is necessary for the conversion of the soluble active form to an inactive phase-separated form of ELF3 at high ambient temperature (Jung et al , 2020). In addition, similar to the bacterial RNA thermometer, an RNA thermoswitch within the 5’-untranslated region of PHYTOCHROME INTERCTING FACTOR 7 (PIF7) acts as a temperature sensor to regulate the translational efficiency of PIF7 mRNA (Chung et al , 2020).…”

Section: Introductionmentioning

confidence: 99%

“…TIMING OF CAB EXPRESSION 1 (TOC1) suppresses thermoresponsive growth in the evening by inhibiting PIF4 activity (Zhu et al , 2016a). EARLY FLOWERING 4 (ELF4) stabilizes ELF3 function, which inhibits PIF4 activity independent of the circadian clock to regulate thermomorphogenesis (Box et al , 2015; Jung et al , 2020; Nieto et al , 2015). Furthermore, GIGANTEA (GI) represses thermomorphogenesis by stabilizing REPRESSOR OF ga1-3 (RGA), which inhibits PIF4 activity (Park et al , 2020).…”

Section: Introductionmentioning

confidence: 99%

An autoregulatory negative feedback loop controls thermomorphogenesis in Arabidopsis

Lee

Zhu

2021

Preprint

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Plant growth and development are acutely sensitive to high ambient temperature ascribable in part to climate change. However, the mechanism of high ambient temperature signaling is not well defined. Here, we show that HECATEs (HEC1 and HEC2), two helix-loop-helix transcription factors, inhibit thermomorphogenesis. While the expression of HEC1 and HEC2 is increased and HEC2 protein is stabilized at high ambient temperature, hec1hec2 double mutant showed exaggerated thermomorphogenesis. Analyses of the four major PIF (PIF1, PIF3, PIF4 and PIF5) mutants and overexpression lines showed that they all contribute to promote thermomorphogenesis. Furthermore, genetic analysis showed that pifQ is epistatic to hec1hec2. HECs and PIFs oppositely control the expression of many genes in response to high ambient temperature. HEC2 interacts with PIF4 both in yeast and in vivo. In the absence of HECs, PIF4 binding to its own promoter as well as the target gene promoters was enhanced, indicating that HECs control PIF4 activity via heterodimerization. Overall, these data suggest that PIF4-HEC forms an autoregulatory composite negative feedback loop that controls growth genes to modulate thermomorphogenesis.

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A prion-like domain in ELF3 functions as a thermosensor in Arabidopsis

Cited by 366 publications

References 24 publications

Microbiome-Dependent Adaptation of Seaweeds Under Environmental Stresses: A Perspective

Microbiome-Dependent Adaptation of Seaweeds Under Environmental Stresses: A Perspective

Molecular pathways regulating elongation of aerial plant organs: a focus on light, the circadian clock, and temperature

An autoregulatory negative feedback loop controls thermomorphogenesis in Arabidopsis

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