A primary screen for drugs to prevent transmission of Chagas's disease during blood transfusion

Cover, B.; Gutteridge, W. E.

doi:10.1016/0035-9203(82)90228-0

Cited by 19 publications

(9 citation statements)

References 13 publications

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“…Gentian violet, introduced more than 40 years ago, has been used to sterilize blood products; however, concerns about this agent include potential mutagenicity, carcinogenicity, toxic effects on erythrocytes, and the acceptability of "purplish-blood" by patients (7). New compounds with trypanosomicidal activity have been sought to sterilize blood products (4,6,7,9,13,15,27). Ideally, candidate compounds will be active at 4ЊC within 24 h to conform with handling procedures of blood products (14).…”

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confidence: 99%

Efficient technique for screening drugs for activity against Trypanosoma cruzi using parasites expressing beta-galactosidase

Buckner

Verlinde

Flamme

et al. 1996

Antimicrob Agents Chemother

479

418

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A new drug screening method was devised utilizing Trypanosoma cruzi cells that express the Escherichia coli ␤-galactosidase gene. Transfected parasites catalyze a colorimetric reaction with chlorophenol red ␤-D-galactopyranoside as substrate. Parasite growth in the presence of drugs in microtiter plates was quantitated with an enzyme-linked immunosorbent assay reader. The assay was performed with the mammalian form of T. cruzi that requires intracellular growth on a monolayer of fibroblast cells. To determine if selective toxicity to the parasites was occurring, the viability of the host cells in the drug was assayed with AlamarBlue. The drugs benznidazole, fluconazole, and amphotericin B were shown to inhibit the parasites at concentrations similar to those previously reported. Several compounds were tested that are inhibitors of glyceraldehyde-3-phosphate dehydrogenase of the related organisms Leishmania mexicana and Trypanosoma brucei. One of these compounds, 2-guanidino-benzimidazole, had an 50% inhibitory concentration of 10 M in our assay. Two derivatives of this compound were identified with in vitro activity at even lower concentrations. In addition, the assay was modified for testing compounds for lytic activity against the bloodstream form of the parasite under conditions used for storing blood products. Thus, an assay with ␤-galactosidase-expressing T. cruzi greatly simplifies screening drugs for selective anti-T. cruzi activity, and three promising new compounds have been identified.

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confidence: 99%

Efficient technique for screening drugs for activity against Trypanosoma cruzi using parasites expressing beta-galactosidase

Buckner

Verlinde

Flamme

et al. 1996

Antimicrob Agents Chemother

479

418

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“…In vitro toxoplasmicidal studies were carried out using the method described by Cover and Gutteridge [21]. Briefly, 45 μl of tachyzoites suspension containing 10 6 cells/ml were incubated, with 5 mL of each fractions/clindamycin (dissolved in DMSO [1%w/v] at final concentration of 1.56–100 μg/ml) [22] at 37°C in 96 wells micro plates.…”

Section: Methodsmentioning

confidence: 99%

In vitro Anti-Toxoplasma gondii Activity of Root Extract/Fractions of Eurycoma longifolia Jack

Kavitha

Noordin

Chan

et al. 2012

BMC Complement Altern Med

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Background Toxoplasma gondii infection causes toxoplasmosis, an infectious disease with worldwide prevalence. The limited efficiency of drugs against this infection, their side effects and the potential appearance of resistant strains make the search of novel drugs an essential need. We examined Eurycoma longifolia root extract and fractions as potential sources of new compounds with high activity and low toxicity. The main goal of this study was to investigate the anti- T. gondii activity of crude extract (TACME) and four fractions (TAF 273, TAF 355, TAF 191 and TAF 401) from E. longifolia , with clindamycin as the positive control. Methods In vitro toxoplasmacidal evaluation was performed using Vero cells as host for T. gondii . Light microscopy technique was used to study in situ antiparasitic activity. Results Significant anti- T. gondii activity was observed with clindamycin (EC50 = 0.016 μg/ml), follow by TAF 355 (EC50 = 0.369 μg/ml) and TAF 401 (EC50 = 0.882 μg/ml). Light microscopy revealed that most Vero cells were infected after 3 h of exposure to T. gondii . After 36 h of exposure to the E. longifolia fraction, the host Vero cells showed no visible intracellular parasite and no remarkable morphological changes. Conclusions Our study demonstrated that TAF 355 and TAF401 fractions may be the sources of new anti- T. gondii compounds.

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“…The in vitro trypanocidal activity o f natural products and extracts was determined following the technique described by Cover and Gutteridge (1982).…”

Section: Methodsmentioning

confidence: 99%

In vitro activity of natural products against the trypomastigote form of Trypanosoma cruzi

González

Sagua

Araya

et al. 1990

Phytotherapy Research

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The activities of isolated compounds and crude extracts from species of the autochthonous flora of the North of Chile, were evaluated against Trypanosoma cruzi trypomastigotes in uitro. A marked trypanocidal activity was observed for the chloroform, ethanol and methanol-aqueous extracts of Gelidium pusilum (Gelidiacea). Similar activity was found in the chloroform extract of Miroglia grandis (Gigartinaceae) and in the methanol + water + chloroform extract of Bacharis boliuiensis (Compositae). The most active extracts tested were p-cumaroiloxitremethone isolated from Parestrephia quadrangularis and two terpenes from Bocharis boliuiensis, not yet structurally elucidated (PN-3 and PN-59). The screen identifies natural products which are active against blood trypomastigotes in uitro at 4°C and which may be useful in preventing the transmission of Chagas' disease by blood transfusion.

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A primary screen for drugs to prevent transmission of Chagas's disease during blood transfusion

Cited by 19 publications

References 13 publications

Efficient technique for screening drugs for activity against Trypanosoma cruzi using parasites expressing beta-galactosidase

Efficient technique for screening drugs for activity against Trypanosoma cruzi using parasites expressing beta-galactosidase

In vitro Anti-Toxoplasma gondii Activity of Root Extract/Fractions of Eurycoma longifolia Jack

In vitro activity of natural products against the trypomastigote form of Trypanosoma cruzi

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