A Preserved Leukocyte Preparation for Quality Control

Abstract

Pinthanond, Damrong, and Petchclai, Bencha: A preserved leukocyte preparation for quality control. Am. J. Clin. Pathol. 63: 32-34, 1975. A preparation technic for stabilized leukocytes for quality control is described. Human leukocytes are separated from 5 ml. of blood using dextran; the erythrocytes are lysed with isotonic NH 4 C1-Tris buffer, pH 7.2. The washed leukocytes are resuspended in 50 ml. of cacodylate HCl buffer, pH 7.4. Five ml. of 2.5% glutaraldehyde were slowly added, and preservation is accomplished by storage overnight at 4 C. The washed leukocytes are resuspended in physiologic saline solution containing 1:1,000 sodium azide, then stored at 4 C and at room temperature. The preparations were highly stable at both temperatures for at least 3 months, as indicated by both manual and electronic leukocyte counts. (Key words: Quality control; Preserved leukocyte; Leukocyte count.) STANDARD LEUKOCYTE suspensions are needed as quality control for accurate electronic leukocyte counting. Latex particle, 3,7 pollen, 3 and commercial preparations of unspecified origin have been used for this purpose. A suspension of human leukocytes if made to be highly stable during simple and prolonged storage should serve the purpose well. The suspension is more similar to routine blood specimens, as the cells are similar in shape, size and composition. We describe here the method to prepare a stabilized leukocyte suspension which fits most of the requirements for quality control in blood cell counting.

Correlation of Mutational Analysis To Clinical Features in Taiwanese Patients With Gilbertʼs Syndrome

“…Genomic DNA was extracted from peripheral blood leukocytes by using a standard method (35). Approximately 50 ng of genomic DNA was used in PCR for amplification of the UGT1A1 promoter region containing the TATAA element.…”