A preliminary study on epigenetic changes during boar spermatozoa cryopreservation

Zeng, Changjun; Peng, Wenpei; Ding, Li; Ling, He; Zhang, Yan; Fang, Donghui; Tang, Keyi

doi:10.1016/j.cryobiol.2014.06.003

Cited by 61 publications

(59 citation statements)

References 88 publications

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“…Therefore, compared with fresh semen, the use of post-thawed boar spermatozoa results in about 10-25% reduced conception or farrowing rates and 2-3 decreased total litter size (Zeng et al, 2014). This constrains the frozenthawed semen to be used usually only in case of transferring valuable genetic material between swine breeding centers (Roca et al, 2011) and maintains the preservation in liquid state, at room temperature (15-20°C) as the standard method in case of boar semen.…”

Section: Correlations Between Some Parameters Of Raw and Stored Semenmentioning

confidence: 99%

Correlations between some Parameters of Raw and Stored Semen in Boar

et al. 2015

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The preservation in liquid form, at 17°C for several days is still the most used method of semen preservation in boar. In these conditions, it would be of great value to know the factors that can predict the capacity of an ejaculate to maintain its biological value over preservation period. This study aimed to identify the correlations between some parameters of raw semen, and those of extended semen, stored in liquid form, for three days at 17°C. Over 1000 ejaculates were collected by means of manual method and artificial vagina over a period of two years from 400 mature boars housed in a specialized unit in Germany. The semen was examined immediately after collection (before dilution) as well as after three days of preservation. All the parameters (except the volume) were determined by means of computerized instruments. The examination of raw semen was performed using SQA-Vp, while the stored semen was analyzed using Spermvision version 3.7. All data were processed with IBM SPSS Statistics version 21. The Pearson correlation revealed no strong correlation between any of the raw semen parameters when analysed together with stored semen parameters. However, some moderate or weak correlations were found, for example a positive correlation between motility of raw semen and motility of stored semen (p<0.01), a positive correlation between percentage of morphological normal sperm in raw semen and the motility of stored semen (p<0.01), a negative correlation between the concentration of raw semen and the linearity and wobble coefficient in stored semen (p<0.01) as well as a couple other weak correlations. We conclude that, despite the fact that some correlations were revealed, unfortunately the analyzed parameters of raw semen have low predictive value on the parameters of extended semen after preservation at 17°C, and that the parameters of semen will behave differently from one ejaculate to another.

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Section: Correlations Between Some Parameters Of Raw and Stored Semenmentioning

confidence: 99%

Correlations between some Parameters of Raw and Stored Semen in Boar

et al. 2015

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“…The resolution of RNA population has been optimized with the utilization of next-generation sequencing (NGS) technology, to uncover complete transcript proiles of mammalian spermatozoa [12], making signiicant contributions to elucidate the physiological roles of sperm-derived RNAs. Moreover, the wider adaption of RNA-Seq has revealed a complex RNA repertoire in spermatozoa from diferent animal species, including the bull [17, 51-53, 55, 56], boar [46][47][48], and stallion [54]. However, despite the increasingly wide applications of RNA-Seq in the analysis of diferent cellular tissues, its application is limited to screening of the mRNA proiles of frozen-thawed spermatozoa to uncover candidate genes associated with semen freezability.…”

Section: Transcriptome Studies On Sperm-derived Rnasmentioning

confidence: 99%

“…Among others, increasing focus has been given to the examination of the biological functions of mRNAs in spermatozoa of diferent animal species. It has been suggested that the analysis of the sperm-derived RNAs might provide potential links between the sperm proteome and semen freezability [17,18,20,[46][47][48]. It should be underlined that the isolation of high-quality RNAs from fresh or frozen-thawed semen is important to assess the sperm gene expression [19,46,49,50].…”

Section: Transcriptome Studies On Sperm-derived Rnasmentioning

confidence: 99%

“…Presently, human sperm transcripts are the best characterized among all mammals with respect to RNA sequence proiling. Even though microarray techniques, coupled with either quantitative real-time qPCR or qRT-PCR, have revealed limited features of the transcriptome and global paterns of gene expression in spermatozoa, these techniques have revealed that sperm transcripts afecting diferent metabolic pathways are related to semen fertility [48,49,[51][52][53][54]. A study based on the evaluation of sperm capacitation status provides evidence, indicating that the sperm-derived RNAs can be translated de novo using mitochondrial-type ribosomes, and at least 26 such sperm-translated proteins are known to be required during capacitation, sperm-egg interactions, and fertilization [11,54].…”

Section: Transcriptome Studies On Sperm-derived Rnasmentioning

confidence: 99%

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Markers for Sperm Freezability and Relevance of Transcriptome Studies in Semen Cryopreservation: A Review

Fraser¹

2017

Theriogenology

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Advances in sperm assessment techniques have ofered new perspectives to improve the technology of semen cryopreservation. This review addresses some recent achievements in the proteomics of seminal plasma and spermatozoa and exempliies its importance as markers for sperm fertility following cryopreservation. Recent advances in transcriptome studies on sperm RNA-Seq data have generated new information aimed to unravel the physiological roles of RNAs in the sperm-egg fertilization processes and their associations with male fertility. The relevance of the sperm freezability markers and the potential associations of RNA-proiling sequences with the sperm biological functions have been discussed.

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“…However, AI utilizing cryopreserved semen accounts for less than 1% of total inseminations performed due to low conception rates and reduced litter sizes (Zeng et al, 2014). This could be due to the process of cooling, freezing, and thawing, causing physical and chemical stress on sperm membranes, which diminishes sperm viability and fertilizing ability (Gadea et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Effect of trans-10, cis-12 isomer of conjugated linoleic acid on boar semen quality after cryopreservation

2017

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The use of frozen semen in pig industry is limited by problems with viability and fertility compared to cooled semen. Part of the decrease in motility and fertility, associated to cryopreservation, may be due to oxidative damage from excessive formation of reactive oxygen species (ROS). Frozen thawed boar spermatozoa are still considered suboptimal due to the low conception rates and smaller litters after artificial insemination. The relatively low fertility of frozen thawed boar semen is associated with many factors including cytotoxicity of the cryoprotectant, osmotic stress, injuries due to ice formation during freezing and thawing, cold shock damages and even inter and intra variations present among boars. Therefore, this study was conducted to determine the impact of conjugated linoleic acid (trans-10, cis-12; CLA) supplementation in the cryopreservation extender frozen-thawed boar on semen quality parameters. Semen was collected from three boars (three ejaculates per boar) which were subjected to cryopreservation, without any supplementation (control) or supplemented with 50 µm CLA, and then the semen was frozen using a controlled rate freezer. Before freezing, and after thawing, the sperm motility was assessed, microscopically and viability and acrosome integrity were assessed using the flow cytometry technique. Regarding live spermatozoa, no significant differences (P > 0.05) were observed among treatments. However, statistical differences (P < 0.05) were found between refrigerated and frozen-thawed semen. Both sperm viability and motility diminished after thawing. Significant differences (P < 0.05) in motility were found not only between refrigerated semen and frozen-thawed group, but also between treatments. In acrosome integrity, no significant differences (P > 0.05) were observed among treatments. In conclusion, the addition of trans-10, cis-12 isomer of conjugated linoleic acid, in the concentration used in the cryopreservation media, showed no advantages on the post-thaw boar sperm viability and integrity.

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A preliminary study on epigenetic changes during boar spermatozoa cryopreservation

Cited by 61 publications

References 88 publications

Correlations between some Parameters of Raw and Stored Semen in Boar

Correlations between some Parameters of Raw and Stored Semen in Boar

Markers for Sperm Freezability and Relevance of Transcriptome Studies in Semen Cryopreservation: A Review

Effect of trans-10, cis-12 isomer of conjugated linoleic acid on boar semen quality after cryopreservation

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