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Background Inhibiting ROS overproduction is considered a very effective strategy for the treatment of peripheral nerve injuries, and Se has a remarkable antioxidant effect; however, since the difference between the effective concentration of Se and the toxic dose is not large, we synthesized a nanomaterial that can release Se slowly so that it can be used more effectively. Methods Se@SiO 2 NPs were synthesized using a mixture of Cu 2-x Se nanocrystals, and the mechanism of action of Se@SiO 2 NPs was initially explored by performing sequencing, immunofluorescence staining and Western blotting of cellular experiments. The mechanism of action of Se@SiO 2 NPs was further determined by performing behavioral assays after animal experiments and by sampling the material for histological staining, immunofluorescence staining, and ELISA. The effects, mechanisms and biocompatibility of Se@SiO 2 NPs for peripheral nerve regeneration were determined. Results Porous Se@SiO 2 was successfully synthesized, had good particle properties, and could release Se slowly. CCK-8 experiments revealed that the optimal experimental doses were 100 μM H 2 O 2 and 200 μg/mL Se@SiO 2 , and RNA-seq revealed that porous Se@SiO 2 was associated with cell proliferation, apoptosis, and the PI3K/AKT pathway. WB showed that porous Se@SiO 2 could increase the expression of cell proliferation antigens (PCNA and S100) and antiapoptotic proteins (Bcl-2), decrease the expression of proapoptotic proteins (Bax), and increase the expression of antioxidative stress proteins (Nrf2, HO-1, and SOD2). EdU cell proliferation and ROS fluorescence assays showed that porous Se@SiO2 promoted cell proliferation and reduced ROS levels. The therapeutic effect of LY294002 (a PI3K/AKT pathway inhibitor) was decreased significantly and its effect was lost when it was added simultaneously with porous Se@SiO 2 . Animal experiments revealed that the regenerated nerve fiber density, myelin thickness, axon area, gastrocnemius muscle wet-to-weight ratio, myofiber area, sciatic nerve function index (SFI), CMAP, apoptotic cell ratio, and levels of antioxidative stress proteins and anti-inflammatory factors were increased following the administration of porous Se@SiO 2 . The levels of oxidative stress proteins and anti-inflammatory factors were significantly greater in the Se@SiO 2 group than in the PNI group, and the effect of LY294002 was decreased significantly and was lost when it was added simultaneously with porous Se@SiO 2 . Conclusion Se@SiO 2 NPs a...
Background Inhibiting ROS overproduction is considered a very effective strategy for the treatment of peripheral nerve injuries, and Se has a remarkable antioxidant effect; however, since the difference between the effective concentration of Se and the toxic dose is not large, we synthesized a nanomaterial that can release Se slowly so that it can be used more effectively. Methods Se@SiO 2 NPs were synthesized using a mixture of Cu 2-x Se nanocrystals, and the mechanism of action of Se@SiO 2 NPs was initially explored by performing sequencing, immunofluorescence staining and Western blotting of cellular experiments. The mechanism of action of Se@SiO 2 NPs was further determined by performing behavioral assays after animal experiments and by sampling the material for histological staining, immunofluorescence staining, and ELISA. The effects, mechanisms and biocompatibility of Se@SiO 2 NPs for peripheral nerve regeneration were determined. Results Porous Se@SiO 2 was successfully synthesized, had good particle properties, and could release Se slowly. CCK-8 experiments revealed that the optimal experimental doses were 100 μM H 2 O 2 and 200 μg/mL Se@SiO 2 , and RNA-seq revealed that porous Se@SiO 2 was associated with cell proliferation, apoptosis, and the PI3K/AKT pathway. WB showed that porous Se@SiO 2 could increase the expression of cell proliferation antigens (PCNA and S100) and antiapoptotic proteins (Bcl-2), decrease the expression of proapoptotic proteins (Bax), and increase the expression of antioxidative stress proteins (Nrf2, HO-1, and SOD2). EdU cell proliferation and ROS fluorescence assays showed that porous Se@SiO2 promoted cell proliferation and reduced ROS levels. The therapeutic effect of LY294002 (a PI3K/AKT pathway inhibitor) was decreased significantly and its effect was lost when it was added simultaneously with porous Se@SiO 2 . Animal experiments revealed that the regenerated nerve fiber density, myelin thickness, axon area, gastrocnemius muscle wet-to-weight ratio, myofiber area, sciatic nerve function index (SFI), CMAP, apoptotic cell ratio, and levels of antioxidative stress proteins and anti-inflammatory factors were increased following the administration of porous Se@SiO 2 . The levels of oxidative stress proteins and anti-inflammatory factors were significantly greater in the Se@SiO 2 group than in the PNI group, and the effect of LY294002 was decreased significantly and was lost when it was added simultaneously with porous Se@SiO 2 . Conclusion Se@SiO 2 NPs a...
Due to widespread selenium deficiency in food the aim of this study was to evaluate the effectiveness of a new Se(II)-containing organic chemical compound 2-iminoselenazolidin-4-ones (ISeA) in the form of a nanoscale associate (1–5 nm) solution for Swiss chard and komatsuna plants biofortification. Application of the chosen substance as a foliar treatment (2 mg·L−1) and as an additive to a hydroponic nutrient solution (10 mg·L−1) was performed. Both cultures had a high level of Se absorption, distribution and accumulation in leaves two or more times greater than in petioles. Se content in chard petioles (15 mg·L−1) when applying ISeA as a component of the nutrient solution exceeded the accumulation of Se during foliar treatment (9.6 mg·L−1) and the same trend in the komatsuna leaves was observed. When applying ISeA to the nutrient solution, an increase in komatsuna and chard biomass was seen at 36 and 68% and for leaf treatment by 21 and 45%, respectively. For komatsuna and chard an increase in the ratio of dry to fresh weight was also observed to be 27 and 26%, and for foliar treatment—0 and 16%, respectively. Treatments led to increase in chard plants height (7–17%), enlargement of leaves (19–42%), a rise in photosynthetic pigments (20–60%) and anthocyanin (2.9 and 2.2 times) concentration, and for komatsuna—the multiplication of leaves number (28%) and their surface area (27–29%) as well as a rise in the concentration of anthocyanin (1.0 and 1.6 times) with foliar treatment and nutrient solution enrichment.
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