A preliminary metatranscriptomic insight of eggshells conditioning on substrates metabolism during food wastes anaerobic fermentation

Luo, Jingyang; Huang, Wenxuan; Zhang, Qin; Guo, Wen; Xu, Runze; Fang, Fang; Cao, Jiashun; Wu, Yang

doi:10.1016/j.scitotenv.2020.143214

Cited by 33 publications

(8 citation statements)

References 29 publications

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“…Hence, the enzymatic pretreatment was also favored to upregulate the gene expressions involved in hydrolysis. Moreover, in terms of pyruvate metabolism, the relative abundance of PK, which could catalyze the pyruvate into acetyl-CoA, 51 was 0.64 and 0.85‰ in the reactors before and after enzymatic pretreatment, respectively. Moreover, the genes of accA and accC encoding acetyl-CoA carboxylase carboxyl transferase, which were involved in the carbohydrate, energy, and lipid metabolic modules, 51 were also observed to be significantly enhanced after enzymatic pretreatment.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Moreover, in terms of pyruvate metabolism, the relative abundance of PK, which could catalyze the pyruvate into acetyl-CoA, 51 was 0.64 and 0.85‰ in the reactors before and after enzymatic pretreatment, respectively. Moreover, the genes of accA and accC encoding acetyl-CoA carboxylase carboxyl transferase, which were involved in the carbohydrate, energy, and lipid metabolic modules, 51 were also observed to be significantly enhanced after enzymatic pretreatment. Hence, the enzymatic pretreatment could upregulate the gene expressions involved in hydrolysis, pyruvate metabolism, and fatty acid biosynthesis, thereby contributing to the VFA generation.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…In this work, the carbohydrate metabolism was observed to be slightly increased from 10.3% in the control to 11.5% in the reactor with pretreatment (Table ). Meanwhile, the relative abundance of membrane transport, which was responsible for delivering extracellular substrates into the cell and was also believed to be positively correlated with VFA generation, increased from 3.0 to 4.6% after enzymatic pretreatment. However, the variation of lipid metabolism was insignificant, which might be ascribed to the relatively difficult degradation process and the relatively low lipid content compared with carbohydrate and protein .…”

Section: Resultsmentioning

confidence: 99%

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Enhanced Volatile Fatty Acid Production from Food Waste Fermentation via Enzymatic Pretreatment: New Insights into the Depolymerization and Microbial Traits

Zhu

et al. 2022

ACS EST Eng.

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Food waste (FW) has been considered a potential carbon reservoir and can be used for high-value volatile fatty acid (VFA) production via anaerobic fermentation. Nevertheless, more work is needed to unravel how to regulate the structural defects of FW and enhance microbial metabolisms for improving VFA production through pretreatment methods. This work investigated the enhancement of VFA production from FW fermentation through enhancing depolymerization and stimulating microbial traits via enzymatic pretreatment. The results exhibited that, after pretreatment, VFA production was improved by 50.1% compared to the unpretreated group, and the concentrations of bioavailable substrates (e.g., reducing sugar and soluble chemical oxygen demand) increased significantly. Further analysis proved that the enzymatic pretreatment could highly reduce the particle sizes of FW by 69.3% and thus promoted the solubilization of substrates and lipids. Meanwhile, the ratio of α-helix/(β-sheet + random coil) in the protein secondary structure of FW decreased from 0.32 to 0.23 after enzymatic pretreatment, indicating that the pretreatment process accelerated the FW depolymerization. Furthermore, the organic substrates after the pretreatment were favorable for enriching the bacteria involved in hydrolysis and acidification (e.g., Olsenella sp. and Sporanaerobacter sp.), upregulating the gene expressions involved in hydrolysis, pyruvate metabolism, and fatty acid biosynthesis (e.g., malZ, PK, accA, and accC), and stimulating the enzymatic activities involved in acetate and butyrate formation (e.g., AK, PTA, BK, and PTB), which was in accordance with the enhanced VFA generation from FW fermentation via enzymatic pretreatment.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Enhanced Volatile Fatty Acid Production from Food Waste Fermentation via Enzymatic Pretreatment: New Insights into the Depolymerization and Microbial Traits

Zhu

et al. 2022

ACS EST Eng.

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“…Apart from the microbial community, methane production in anaerobic digestion is also tightly related to bacterial and archaeal enzyme activity. , In this study, the activities of protease/α-glucosidase (nonspecific extracellular enzymes), AK, FTHFS, ACAS, and mcrA in the long-term test were investigated to represent the capability of hydrolysis, acidogenesis, homoacetogenesis, acetate-dependent methanogenesis, and all-pathway methanogenesis, respectively (Figure ). As shown in Figures and S6, the activities of protease and α-glucosidase were significantly inhibited by chitosan at any level investigated ( p < 0.05), indicating that chitosan caused decreases in transformation of proteins and polysaccharides into their microforms (e.g., amino acid and glucose) for the subsequent utilization by acetogens and methanogens.…”

Section: Resultsmentioning

confidence: 99%

How Does Chitosan Affect Methane Production in Anaerobic Digestion?

Liu

et al. 2021

Environ. Sci. Technol.

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The expanding use of chitosan in sewage and sludge treatment processes raises concerns about its potential environmental impacts. However, investigations of the impacts of chitosan on sewage sludge anaerobic digestion where chitosan is present at substantial levels are sparse. This study therefore aims to fill this knowledge gap through both long-term and batch tests. The results showed that 4 g/kg total suspended solid (TSS) chitosan had no acute effects on methane production, but chitosan at 8–32 g/kg TSS inhibited methane production by 7.2–30.3%. Mass balance and metabolism of organic analyses indicated that chitosan restrained the transfer of organic substrates from solid phase to liquid phase, macromolecules to micromolecules, and finally to methane. Further exploration revealed that chitosan suppressed the secretion of extracellular polymeric substances of anaerobes by occupying the connection sites of indigenous carbohydrates and increased the mass transfer resistance between anaerobes and substrates, which thereby lowered the metabolic activities of anaerobes. Although chitosan could be partly degraded by anaerobes, it is much more persistent to be degraded compared with indigenous organics in sludge. Microbial community and key enzyme encoding gene analyses further revealed that the inhibition of chitosan to CO2-dependent methanogenesis was much severer than that to acetate-dependent methanogenesis.

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“…Recently, J. Luo et al also investigated the effect of eggshells on food waste fermentation for VFA production by utilizing a metatranscriptomics approach [ 104 ]. The authors analyzed the difference between the gene expression with and without eggshell conditioning and observed an enhancement in VFA production by 12 times.…”

Section: Metagenomics and Metatranscriptomics In Acidogenic Fermentationmentioning

confidence: 99%

Next-generation -omics approaches to drive carboxylate production by acidogenic fermentation of food waste: a review

Kumar

Brar

et al. 2022

Bioengineered

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Acidogenic fermentation of food waste using mixed microbial cultures can produce carboxylates [or volatile fatty acids (VFA)] as high-valued bioproducts via a complex interplay of microorganisms during different stages of this process. However, the present fermentation systems are incapable of reaching the industrially relevant VFA production yields of ≥50 g/L primarly due to the complex process operation, competitive metabolic pathways, and limited understanding of microbial interplays. Recent reports have demonstrated the significant roles played by microbial communities from different phyla, which work together to control the process kinetics of various stages underlying acidogenic fermentation. In order to fully delineate the abundance, structure, and functionality of these microbial communities, next-generation high-throughput meta-omics technologies are required. In this article, we review the potential of metagenomics and metatranscriptomics approaches to enable microbial community engineering. Specifically, a deeper analysis of taxonomic relationships, shifts in microbial communities, and differences in the genetic expression of key pathway enzymes under varying operational and environmental parameters of acidogenic fermentation could lead to the identification of species-level functionalities for both cultivable and non-cultivable microbial fractions. Furthermore, it could also be used for successful gene sequence-guided microbial isolation and consortium development for bioaugmentation to allow VFA production with high concentrations and purity. Such highly controlled and engineered microbial systems could pave the way for tailored and high-yielding VFA synthesis, thereby creating a petrochemically competitive waste-to-value chain and promoting the circular bioeconomy.Research HighlightsMixed microbial mediated acidogenic fermentation of food waste.Metagenomics and metatranscriptomics based microbial community analysis.Omics derived function-associated microbial isolation and consortium engineering.High-valued sustainable carboxylate bio-products, i.e. volatile fatty acids.

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A preliminary metatranscriptomic insight of eggshells conditioning on substrates metabolism during food wastes anaerobic fermentation

Cited by 33 publications

References 29 publications

Enhanced Volatile Fatty Acid Production from Food Waste Fermentation via Enzymatic Pretreatment: New Insights into the Depolymerization and Microbial Traits

Enhanced Volatile Fatty Acid Production from Food Waste Fermentation via Enzymatic Pretreatment: New Insights into the Depolymerization and Microbial Traits

How Does Chitosan Affect Methane Production in Anaerobic Digestion?

Next-generation -omics approaches to drive carboxylate production by acidogenic fermentation of food waste: a review

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