A preliminary investigation into the genetic variation and population structure of Taenia hydatigena from Sardinia, Italy

Boufana, Belgees; Scala, Antonio; Lahmar, Samia; Pointing, S; Craig, Philip S.; Dessì, Giorgia; Zidda, Antonella; Pipia, Anna Paola; Varcasia, Antonio

doi:10.1016/j.vetpar.2015.08.003

Cited by 36 publications

(47 citation statements)

References 38 publications

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“…The number of haplotypes based on the cox1 gene was lower compared to the number of haplotypes (4) reported among a T. hydatigena population of Tanzanian sheep and goats [4] but similar to the number of haplotypes reported in Nigerian sheep [18]. The comparison of the nucleotide sequences from this study with the deposited sequences in the GenBank database yielded no haplotype with 100% identity, which could suggest the presence of unique haplotypes in Sudan and is consistent with previous reports of the existence of geographically distinct species of T. hydatigena [29]. Nonetheless, with limited sample size and the use of short mitochondrial DNA (mtDNA) fragments, further investigation would be necessary to understand and clearly describe the genetic differences and relationships between Sudanese T. hydatigena isolates and those from different geographical regions.…”

supporting

confidence: 86%

“…Low haplotype (Hd) and nucleotide (π) diversities were observed (Table 1). Regardless of small sample size, the observed nad1 Hd was comparable to T. hydatigena populations reported in dogs and goats, although with a lower π compared to the T. hydatigena populations from the same study [29]. A comparison of the cox1 diversity indices also revealed lower Hd and π than the populations from Italian, Iranian, and Palestinian sheep [29].…”

supporting

confidence: 68%

“…Regardless of small sample size, the observed nad1 Hd was comparable to T. hydatigena populations reported in dogs and goats, although with a lower π compared to the T. hydatigena populations from the same study [29]. A comparison of the cox1 diversity indices also revealed lower Hd and π than the populations from Italian, Iranian, and Palestinian sheep [29]. Meanwhile, comparable data from an African host on the diversity indices of T. hydatigena are lacking [4,17].…”

supporting

confidence: 57%

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Molecular Identification of Taenia hydatigena from Sheep in Khartoum, Sudan

et al. 2020

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The cestode Taenia hydatigena uses canids, primarily dogs, as definitive hosts, while the metacestode larval stage cysticercus infects a range of intermediate hosts, including domestic animals such as goats, sheep, and pigs. Cysticercosis due to T. hydatigena has large veterinary and economic drawbacks. Like other taeniids, e.g., Echinococcus, intraspecific variation is found among the members of the genus Taenia. In Africa, few studies are available on the epidemiology and distribution of T. hydatigena, and even fewer studies are available on its genetic variation. In this study, we molecularly identified 11 cysticerci from sheep in Sudan and demonstrated the genetic variation based on the NADH dehydrogenase subunit 1 (nad1) and cytochrome c oxidase subunit 1 (cox1) mitochondrial genes. The isolates were correctly identified as T. hydatigena with more than 99% similarity to those in the GenBank database. Low diversity indices and insignificant neutrality indices were observed, with 3 and 2 haplotypes for the nad1 and cox1 genes, respectively. The results suggest the presence of unique T. hydatigena haplotypes in Sudan, as haplotypes with 100% similarity were not found in the GenBank database. With few available studies on the genetic variation of T. hydatigena in Africa, this report represents the first insights into the genetic variation of T. hydatigena in Sudan and constitutes useful data.

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supporting

confidence: 86%

supporting

confidence: 68%

supporting

confidence: 57%

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Molecular Identification of Taenia hydatigena from Sheep in Khartoum, Sudan

et al. 2020

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“…As shown in Table 2 , maximum number of microsatellites in ITS1 region was found in T. hydatigena and T. multiceps followed by T. saginata and E. granulosus sensu stricto. Taenia hydatigena has previously been shown to have a high degree of variability in different regions of the world 33 , 34 probably because of the highest prevalence and intensity of T. hydatigena transmission among the four taeniid species in this study 5 - 7 .…”

Section: Discussionmentioning

confidence: 67%

A conventional PCR for differentiating common taeniid species of dogs based on in silico microsatellite analysis

Shamsaddini

Mohammadi

Mirbadie

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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Canine taeniids are among the major tapeworms with remarkable medical and economic significance. Reliable diagnosis and differentiation of dog taeniids using simple and sensitive tools are of paramount importance for establishing an efficient surveillance system. Microsatellites as abundant unique tandem repeats of short DNA motifs are useful genetic markers for molecular epidemiological studies. The purpose of the present study was to find a primer pair for rapid differentiation of major tapeworms of dogs, Taenia hydatigena, T. multiceps, T. ovis and Echinococcus granulosus, by screening existing nucleotide data. All the mitochondrial genome records as well as non-coding ITS1 sequences of Taeniidae species were downloaded from Nucleotide database from NCBI. For prediction and analysis of potential loci of STR/SSR in ITS1 as well as mitochondrial regions, we used ChloroMitoSSRDB 2.0 and GMATo v1.2. software. Different tapeworm species were categorized according to different motif sequences and type and size of each microsatellite locus. Three primer sets were designed and tested for differentiating taeniid species and evaluated in a conventional PCR system. Four taeniid species were successfully differentiated using a primer pair in a simple conventional PCR system. We predicted 2-19 and 1-4 microsatellite loci in ITS1 and mitochondrial genome, respectively. In ITS1, 41 Di and 21 Tri motifs were found in the taeniids while the majority of the motifs in the mitochondrial genome were Tetra (89) and Tri (70). It is documented that the number and diversity of microsatellite loci is higher in nuclear ITS1 region than mostly coding mitochondrial genome.

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“…Identical sequences of coenurus cerebralis were also found in different hosts and different body parts: cox1 sequences (396bp) in the muscle of a goat (KT253993) and in the brain of a sheep (KT253934) [ 19 ]. In an investigation of the genetic variation and population structure of cysticercus tenuicollis from intermediate hosts, cysticercus tenuicollis isolates from sheep were observed to be differentiated from those of goat and pig origin at the nad1 locus; in addition, the goat-derived isolates were genetically different from adult T. hydatigena as indicated by the statistically significant Fst value [ 4 ]. Whether sequence differences are related to clinical features and/or organ preference needs to be explored in the future by analyzing other coenurus cerebralis and cysticercus tenuicollis cysts.…”

Section: Resultsmentioning

confidence: 99%

Genetic characterization of three mitochondrial gene sequences of goat/sheep-derived coenurus cerebralis and cysticercus tenuicollis isolates in Inner Mongolia, China

et al. 2018

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Taenia multiceps and Taenia hydatigena are widely distributed tapeworms of canids. Due to a lack of genetic information on these two parasites in China, in this study we analyzed six coenurus cerebralis and two cysticercus tenuicollis cysts from goats or sheep in Inner Mongolia, northern China by amplifying three mitochondrial genes (cox1, nad4, and cytb). Two haplotypes were obtained at each locus for either of the two Taenia cestode species, with ten nucleotide sequences being novel. The degrees of genetic variations were 1.18%, 0.61% and 0.52% for coenurus cerebralis, and 0.24%, 0.46% and 0.35% for cysticercus tenuicollis at the cox1, nad4 and cytb loci, respectively. This is the first molecular description of animal-derived metacestodes of T. multiceps and T. hydatigena in Inner Mongolia, China. Novel nucleotide sequences might reflect endemic genetic characterization of the two cestodes. The present data are useful to explore the biological and epidemiological significance of intra-specific variations within both Taenia cestodes.

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A preliminary investigation into the genetic variation and population structure of Taenia hydatigena from Sardinia, Italy

Cited by 36 publications

References 38 publications

Molecular Identification of Taenia hydatigena from Sheep in Khartoum, Sudan

Molecular Identification of Taenia hydatigena from Sheep in Khartoum, Sudan

A conventional PCR for differentiating common taeniid species of dogs based on in silico microsatellite analysis

Genetic characterization of three mitochondrial gene sequences of goat/sheep-derived coenurus cerebralis and cysticercus tenuicollis isolates in Inner Mongolia, China

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