A predictive threshold value for the diagnosis of early pregnancy in cows using interferon-stimulated genes in granulocytes

Yoshino, Hitomi; Toji, Noriyuki; Sasaki, Kouya; Koshi, K.; Yamagishi, Norio; Takahashi, Toshio; Ishiguro‐Oonuma, Toshina; Matsuda, Hideo; Yamanouchi, T.; Hashiyada, Y.; Imai, Kyoichi; Izaike, Yoshiaki; Kizaki, Keiichiro; Hashizume, Kazuyoshi

doi:10.1016/j.theriogenology.2017.11.014

Cited by 34 publications

(34 citation statements)

References 44 publications

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“…Several authors, however, have proposed expression of ISG in peripheral blood leukocytes as a possible tool for early pregnancy diagnosis in ruminants ( Green et al., 2010 ; Pugliesi et al., 2014 ). This technique has been validated in total leukocytes ( Han et al., 2006 ; Stevenson et al., 2007 ; Green et al., 2010 ), peripheral blood mononuclear cells ( Gifford et al., 2007 ; Pugliesi et al., 2014 ; Melo et al., 2020b ) and peripheral blood polymorphonuclear cells ( Kizaki et al., 2013 ; Yoshino et al., 2018 ; Melo et al., 2020a ). In general, significant differences between pregnant and non-pregnant females were found between days 18 and 20 of gestation, with accuracy of positive pregnancy diagnosis ranging from 70 to 90% in beef ( Pugliesi et al., 2014 ; Melo et al., 2020a ) and dairy herds ( Yoshino et al., 2018 ).…”

Section: Chemical-based Methods: Non-pregnancy Specificmentioning

confidence: 99%

New approaches to diagnose and target reproductive failure in cattle

et al. 2020

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Reproductive failure and pregnancy loss in cattle are some of the largest economic burdens to cattle producers and one of most perplexing factors influencing management decisions. Pregnancy loss may occur at any point during gestation with the largest percentage of loss occurring in the first 30 days and, subsequently, decreasing as the pregnancy progresses. Losses may be attributed to numerous factors, predisposed issues or environmental conditions such as nutritional stressors or disease. From a research perspective, determining the exact causes of pregnancy loss or embryonic mortality in cattle have been difficult, due to limitations of accurately determining early gestation pregnancy status. Until methods that precisely determine embryo success early in gestation are available, our understanding of in vivo pregnancy loss will lack clarity necessary to develop management strategies to decrease such loss. In this review, we will briefly discuss the pivotal periods of pregnancy loss affecting beef and dairy cattle, methods and technologies to determine pregnancy status and embryo viability and potential opportunities to decrease reproductive failure.

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Section: Chemical-based Methods: Non-pregnancy Specificmentioning

confidence: 99%

New approaches to diagnose and target reproductive failure in cattle

et al. 2020

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“…Results in the present study suggest that the evaluation of luteal function by plasma P 4 assay on Day 23 is not able to forecast the occurrence of pregnancy loss in advance. Several procedures for extra-early pregnancy diagnosis in the cow have been proposed (Gifford et al, 2007;Pohler et al, 2017;Schanzenbach et al, 2017;Yoshino et al, 2018); however, pre-hoc diagnosis of pregnancy loss has not been estab- (Sinedino et al, 2014;Spencer et al, 2018;Wijma et al, 2018). In these studies, non-pregnancy diagnoses have carried out on Day 28 (Sinedino et al, 2014), Day 32 (Wijma et al, 2018), and Day 37 (Spencer et al, 2018), and following re-inseminations were done further 3-8 days later.…”

Section: Discussionmentioning

confidence: 99%

“…Results in the present study suggest that the evaluation of luteal function by plasma P 4 assay on Day 23 is not able to forecast the occurrence of pregnancy loss in advance. Several procedures for extra‐early pregnancy diagnosis in the cow have been proposed (Gifford et al., 2007; Pohler et al., 2017; Schanzenbach et al., 2017; Yoshino et al., 2018); however, pre‐hoc diagnosis of pregnancy loss has not been established yet. Therefore, the positive predictive value never increases up to 100%.…”

Section: Discussionmentioning

confidence: 99%

Development of the Timed Re‐Insemination (TRI‐synch) program re‐inseminating 24 days after the initial service in dairy cows

Aizu

Ido-Matsumoto

Wada

et al. 2020

Animal Science Journal

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For the timed re‐insemination at the minimal interbreeding interval, cows were treated with a progesterone (P4)‐releasing intravaginal device from Days 13–15 to 21 post‐insemination (Day 0 = estrus), followed by plasma P4 assay on Day 23 and then subjected to the Experiments 1 and 2. In Experiment 1, of 18 cows, 6 cows were determined as luteolysis with low (<1 ng/ml) plasma P4 concentrations on Day 23 and ovulated on Days 24 (3 cows), 25 (1 cow), and 26 (1 cow) except a cow affected by ovarian quiescence. In Experiment 2, all cows were treated with GnRH on Day 23. Cows with low (<1 ng/ml) plasma P4 concentrations on Day 23 were diagnosed as non‐pregnant and subjected to the re‐insemination in the morning of Day 24 irrespective of estrous signs. Of 36 cows, 15 cows were diagnosed as being non‐pregnant on Day 23. Fourteen cows of the non‐pregnant animals were re‐inseminated in the morning of Day 24 irrespective of estrous signs and the pregnancy rate of re‐insemination was 36%. The conception rates of initial and re‐inseminations were 50% (18/36) and 36% (5/14), respectively. The overall pregnancy rate by adding the rates of initial and re‐inseminations was 64% (23/36).

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“…Novel methods for early pregnancy diagnosis in cattle have been investigated to detect non-pregnant cows on the third week of pregnancy (Spencer and Bazer, 2004;Hansen et al, 2017), decreasing the interval between timed AI (TAI) services (Ribeiro et al, 2012;Fricke et al, 2016). Although previous studies have indicated that determination of IFN-τ-stimulated genes (ISG) in circulating immune cells can be used as a potential pregnancy diagnostic test in beef (Matsuyama et al, 2012;Pugliesi et al, 2014; and dairy (Shirasuna et al, 2012;Haq et al, 2016) cattle, there is still no method with proven accuracy and feasibility for detection of pregnancy before d 20 of pregnancy (Green et al, 2010;Yoshino et al, 2018;. Many studies have investigated ISG mRNA abundance us-ing peripheral blood mononuclear cells (PBMC).…”

Section: Short Communication Physiologymentioning

confidence: 99%

“…Many studies have investigated ISG mRNA abundance us-ing peripheral blood mononuclear cells (PBMC). Recently, other studies have used total immune cells from whole blood (Yoshino et al, 2018) or milk (Schanzenbach et al, 2017) or cervical and vaginal mucosa cells (Kunii et al, 2018) for accessing gene expression and predicting pregnancy status. In addition, novel potential pregnancy markers were recently reported by our group in beef heifers (Rocha et al, 2020) and need to be tested for early pregnancy diagnosis in dairy cattle.…”

Section: Short Communication Physiologymentioning

confidence: 99%

Feasibility and accuracy of using different methods to detect pregnancy by conceptus-stimulated genes in dairy cattle

et al. 2021

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Interferon-tau (IFN-τ) is a pregnancy recognition signal secreted from the trophectoderm cells of the bovine conceptus that induces the expression of IFN-stimulated genes (ISG) in immune cells. The expression of ISG in peripheral blood leukocytes has been suggested as a potential tool for early pregnancy diagnosis. IFN-τ induces an increase in abundance of several ISG in peripheral mononuclear leukocytes between 18 and 20 days after fixed-time AI. We evaluated the abundance of 2 genes (ISG15 and LGALS3BP) stimulated by the conceptus using samples collected from 4 cell types: peripheral blood mononuclear cells (PBMC), total blood leukocytes, cervical cells, and total milk leukocytes, and we compared these four methods as pregnancy predictors on day 20 after timed AI in dairy cattle.

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A predictive threshold value for the diagnosis of early pregnancy in cows using interferon-stimulated genes in granulocytes

Cited by 34 publications

References 44 publications

New approaches to diagnose and target reproductive failure in cattle

New approaches to diagnose and target reproductive failure in cattle

Development of the Timed Re‐Insemination (TRI‐synch) program re‐inseminating 24 days after the initial service in dairy cows

Feasibility and accuracy of using different methods to detect pregnancy by conceptus-stimulated genes in dairy cattle

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