Due to the higher reliability needs of the large moving component motor-generator rotor, the assessment of the service life has drawn more and more attention. After finite element analysis of the rotor, the simulation part which can represent the magnetic pole with the most dangerous position of the rotor was designed to investigate the S–N curves. Compared with the conventional specimen, considering the main influencing factors of fatigue life for simulation part, the comprehensive factor was proposed to establish the fatigue life relationship between magnetic pole material and simulation part. It was found that the calculation method of fatigue notch factor based on the notch sensitivity factor is relatively simple and practical, and there is no significant effect of surface roughness on high and low cycle fatigues for low roughness ( R a is about 1 µm), and the dimension factor changes linearly with the scale factor. Based on those results, a fatigue life prediction method was proposed and validated, and the predicted results were in good agreement with the experimental data. This study will provide a reasonable reference to determine the fatigue life prediction of large moving components.