A preclinical small animal imaging platform combining multi-angle photoacoustic and fluorescence projections into co-registered 3D maps

Thompson, Weylan; Yu, Anthony M.; Dumani, Diego S.; Cook, Jason R.; Anastasio, Mark A.; Emelianov, Stanislav; Ermilov, Sergey A.

doi:10.1117/12.2549088

Cited by 4 publications

(5 citation statements)

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“…To further demonstrate the clinical translation potential of the JAAZ particles, an in vivo pilot study was conducted on the commercially available small animal PAI system TriTom™ ( Fig. 6 A) [45] , [46] , [47] . We first acquired multiwavelength images of microcuvette tubes containing 0.4 mM of ICG dye or RGD-JAAZ particles to evaluate the PA spectra of both contrast agents.…”

Section: Resultsmentioning

confidence: 99%

“…The phantom and in vivo photoacoustic imaging studies were performed using the TriTom™ imaging platform (PhotoSound Technologies, Inc.) and 20 Hz nanosecond-pulsed OPO laser (PhotoSonus, EKSPLA) located at MD Anderson Cancer Research Center in Houston, TX. The TriTom is a commercially available multimodal imaging technology capable of high-resolution, 3D multispectral photoacoustic imaging in the whole body of small animal models [45] . To prepare for imaging, the TriTom™ imaging chamber was filled with degassed, deionized water at room temperature for phantom experiments or heated to 36.0 ± 0.5 °C for animal studies.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Size-tunable ICG-based contrast agent platform for targeted near-infrared photoacoustic imaging.

Singh

Giammanco

et al. 2023

Photoacoustics

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Size-tunable ICG-based contrast agent platform for targeted near-infrared photoacoustic imaging.

Singh

Giammanco

et al. 2023

Photoacoustics

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“…The dynamic phantom, positioned at the center of the imaging system, was illuminated by a laser pulse with a wavelength of 770 nm and an energy of 100 mJ (before entering the fiber optic light delivery unit). PACT data were acquired with the TriTom imaging system during the injection of a photoacoustic-fluorescent contrast agent (PAtIR 55 ) through one end of the tube. Over a span of 36 s, the dynamic phantom underwent scanning, which resulted in 360 imaging frames in total, with 10 imaging frames per second and a single tomographic measurement per imaging frame.…”

Section: Study Descriptionmentioning

confidence: 99%

Spatiotemporal image reconstruction to enable high-frame-rate dynamic photoacoustic tomography with rotating-gantry volumetric imagers

Cam,

Wang,

Thompson

et al. 2024

J. Biomed. Opt.

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Dynamic photoacoustic computed tomography (PACT) is a valuable imaging technique for monitoring physiological processes. However, current dynamic PACT imaging techniques are often limited to two-dimensional spatial imaging. Although volumetric PACT imagers are commercially available, these systems typically employ a rotating measurement gantry in which the tomographic data are sequentially acquired as opposed to being acquired simultaneously at all views. Because the dynamic object varies during the data-acquisition process, the sequential data-acquisition process poses substantial challenges to image reconstruction associated with data incompleteness. The proposed image reconstruction method is highly significant in that it will address these challenges and enable volumetric dynamic PACT imaging with existing preclinical imagers. Aim:The aim of this study is to develop a spatiotemporal image reconstruction (STIR) method for dynamic PACT that can be applied to commercially available volumetric PACT imagers that employ a sequential scanning strategy. The proposed reconstruction method aims to overcome the challenges caused by the limited number of tomographic measurements acquired per frame.Approach: A low-rank matrix estimation-based STIR (LRME-STIR) method is proposed to enable dynamic volumetric PACT. The LRME-STIR method leverages the spatiotemporal redundancies in the dynamic object to accurately reconstruct a four-dimensional (4D) spatiotemporal image. Results:The conducted numerical studies substantiate the LRME-STIR method's efficacy in reconstructing 4D dynamic images from tomographic measurements acquired with a rotating measurement gantry. The experimental study demonstrates the method's ability to faithfully recover the flow of a contrast agent with a frame rate of 10 frames per second, even when only a single tomographic measurement per frame is available. Conclusions:The proposed LRME-STIR method offers a promising solution to the challenges faced by enabling 4D dynamic imaging using commercially available volumetric PACT imagers. By enabling accurate STIRs, this method has the potential to significantly advance preclinical research and facilitate the monitoring of critical physiological biomarkers.

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“…To further demonstrate the clinical translation potential of the JAAZ particles, an in vivo pilot study was conducted on the commercially available small animal PAI system TriTom™ (Figure 6A) [43][44][45]. We first acquired multiwavelength images of microcuvette tubes containing 0.4 mM of ICG dye or RGD-JAAZ particles to evaluate the PA spectra of both contrast agents.…”

Section: Pilot In-vivo Imaging Of Two Mice Using Rgd Functionalized J...mentioning

confidence: 99%

“…The phantom and in vivo photoacoustic imaging studies were performed using the TriTom™ imaging platform (PhotoSound Technologies, Inc.) and 20 Hz nanosecond-pulsed OPO laser (PhotoSonus, EKSPLA) located at MD Anderson Cancer Research Center in Houston, TX. The TriTom is a commercially available multimodal imaging technology capable of high-resolution, 3D multispectral photoacoustic imaging in the whole body of small animal models [43]. To prepare for imaging, the TriTom™ imaging chamber was filled with degassed, deionized water at room temperature for phantom experiments or heated to 36.0 ± 0.5°C for animal studies.…”

Section: In-vivo Photoacoustic Imaging Using Rgd-jaaz Particlesmentioning

confidence: 99%

Size-tunable ICG-based contrast agent platform for targeted near-infrared photoacoustic imaging

Singh

Giammanco

et al. 2022

Preprint

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Near-infrared photoacoustic imaging (NIR-PAI) combines the advantages of optical and ultrasound imaging to provide anatomical and functional images of tissues with high resolution. Although NIR-PAI is promising, its wide application is hindered by the limited availability of NIR contrast agents. J-aggregates (JA) made of indocyanine green dye (ICG) represent an attractive class of biocompatible contrast agents for PAI. Here, we present a facile synthesis method that combines ICG and ICG-azide dyes for producing contrast agents with tunable size down to 230 nm and allows direct functionalization with targeting moieties. The ICG-JA platform has high photostability and a PA-signal amplitude that is two times stronger than whole blood. The targeting ability of ICG-JA was validated in vitro using HeLa cells. The ICG-JA was then injected into mice and in vivo NIR-PAI showed enhanced visualization of liver and spleen for 90 minutes post-injection with a contrast-to-noise ratio of 2.42.

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A preclinical small animal imaging platform combining multi-angle photoacoustic and fluorescence projections into co-registered 3D maps

Cited by 4 publications

References 0 publications

Size-tunable ICG-based contrast agent platform for targeted near-infrared photoacoustic imaging.

Size-tunable ICG-based contrast agent platform for targeted near-infrared photoacoustic imaging.

Spatiotemporal image reconstruction to enable high-frame-rate dynamic photoacoustic tomography with rotating-gantry volumetric imagers

Size-tunable ICG-based contrast agent platform for targeted near-infrared photoacoustic imaging

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