A precision multi-sampler for deep-sea hydrothermal microbial mat studies

Breier, J. A.; Gomez-Ibañez, Daniel; Reddington, Emily; Huber, Julie A.; Emerson, David

doi:10.1016/j.dsr.2012.10.006

Cited by 34 publications

(38 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Total microbial cell counts in the fluids ranged from 3 × 10 4 to 3 × 10 5 cells ml −1 , with background seawater concentrations of 1–2 × 10 4 cells ml −1 (Table ). Distinct cell morphologies and clusters of cells not visible in background seawater were seen, including large filaments and mineral surfaces colonized by microbial cells (http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13173/suppinfo; Breier et al ., ). These results indicate local enrichment of microbial communities in the venting fluids, distinct from background seawater populations, and are consistent with previous enumerations of microbial cells in venting fluids (e.g.…”

Section: Resultsmentioning

confidence: 97%

“…Low temperature diffuse hydrothermal fluid samples were collected in January 2012 using the ROV Jason II (http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13173/suppinfo) and the Mat Sampler, as described in Breier and colleagues (). This involved measuring the fluid temperature with the Jason temperature probe in regions of hydrothermal fluid flow (http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13173/suppinfo), then positioning the sampler intake into the vent and pumping fluid through either a 0.22 μm Sterivex™‐GP filter (Millipore) or a custom‐made non‐gas‐tight 100 ml flow‐through water bottle.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Subseafloor microbial communities in hydrogen‐rich vent fluids from hydrothermal systems along the Mid‐Cayman Rise

Réveillaud

Reddington

McDermott

et al. 2016

Environmental Microbiology

123

View full text Add to dashboard Cite

SummaryWarm fluids emanating from hydrothermal vents can be used as windows into the rocky subseafloor habitat and its resident microbial community. Two new vent systems on the Mid‐Cayman Rise each exhibits novel geologic settings and distinctively hydrogen‐rich vent fluid compositions. We have determined and compared the chemistry, potential energy yielding reactions, abundance, community composition, diversity, and function of microbes in venting fluids from both sites: Piccard, the world's deepest vent site, hosted in mafic rocks; and Von Damm, an adjacent, ultramafic‐influenced system. Von Damm hosted a wider diversity of lineages and metabolisms in comparison to Piccard, consistent with thermodynamic models that predict more numerous energy sources at ultramafic systems. There was little overlap in the phylotypes found at each site, although similar and dominant hydrogen‐utilizing genera were present at both. Despite the differences in community structure, depth, geology, and fluid chemistry, energetic modelling and metagenomic analysis indicate near functional equivalence between Von Damm and Piccard, likely driven by the high hydrogen concentrations and elevated temperatures at both sites. Results are compared with hydrothermal sites worldwide to provide a global perspective on the distinctiveness of these newly discovered sites and the interplay among rocks, fluid composition and life in the subseafloor.

show abstract

Section: Resultsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Subseafloor microbial communities in hydrogen‐rich vent fluids from hydrothermal systems along the Mid‐Cayman Rise

Réveillaud

Reddington

McDermott

et al. 2016

Environmental Microbiology

123

View full text Add to dashboard Cite

show abstract

“…The DSV Jason II, equipped with a precision microbial mat sampler (Breier et al, 2012), was used for the collection of 231 individual syringes of mat samples across six dives (J2671-J2676). TN293).…”

Section: Study Overview Site Description and Samplingmentioning

confidence: 99%

Bringing microbial diversity into focus: high‐resolution analysis of iron mats from the Lō‘ihi Seamount

Scott

Glazer

Emerson

2017

Environmental Microbiology

View full text Add to dashboard Cite

Thirty kilometers south of the island of Hawai'i lies the Lō'ihi Seamount, an active submarine volcano that hosts a network of low-temperature hydrothermal vents enriched in ferrous iron that supports extensive microbial mats. These mats, which can be a half a meter deep, are composed of ferric iron bound to organic polymers - the metabolic byproduct of iron-oxidizing Zetaproteobacteria. Though the role of Zetaproteobacteria in mat formation is well established, we have a limited understanding of how differences in diversity are related to mat morphology. We used Minimum Entropy Decomposition and ZetaOtu classification to demonstrate cryptic diversity between closely related Zetaproteobacteria while showing habitat and geographic specificity. Veiled mats, common structures at Lō'ihi, exhibit distinct community composition and contain diversity not detected in other mat types, including specific Zetaproteobacteria and an unclassified Gammaproteobacteria. Our analyses also indicate that diversity can change dramatically across small spatial transects from points of active venting, yet we found comparatively few differences between major sampling sites. This study provides a better picture of the microbiome responsible for iron mat production at Lō'ihi and has broad implications for our understanding of these globally distributed communities.

show abstract

“…Installation of new Circulation Obviation Retrofit Kits (CORKs) that have been redesigned and constructed using inert materials such as Teflon-like fluid delivery lines, titanium fittings, and fiberglass borehole casing (e.g., Fisher et al, 2011a; Edwards et al, 2012b; Orcutt et al, 2012) have allowed for collection of high-integrity fluid samples at the seafloor during ROV expeditions (see Wheat et al (2011) for recent review of CORK developments). Additionally, new seafloor instrumentation and subsurface observatory hardware has recently been deployed to enable microbiological investigations (Fisher et al, 2011a; Orcutt et al, 2011b; Edwards et al, 2012b), including an in situ electrochemical analyzer (e.g., Edwards et al, 2011a), in situ fluid pumping systems for collecting pristine fluids and particles from hydrothermal vents, plumes, and microbial mats (Breier et al, 2012), and new integrated sensor and sampling packages for ROVs during short-term sampling, and for automated instrument packages during year-long CORK deployments (Cowen et al, 2012). In the specific case of sampling basement formation fluids from CORK observatories, the capability for collecting large volumes of formation fluid (60 liters per dive) has enabled investigations for biogeochemical activities (Lin et al, 2012) and microbial diversity (Jungbluth et al, 2012).…”

Section: Recent Advances In Marine Deep Biosphere Researchmentioning

confidence: 99%

Microbial activity in the marine deep biosphere: progress and prospects

et al. 2013

View full text Add to dashboard Cite

The vast marine deep biosphere consists of microbial habitats within sediment, pore waters, upper basaltic crust and the fluids that circulate throughout it. A wide range of temperature, pressure, pH, and electron donor and acceptor conditions exists—all of which can combine to affect carbon and nutrient cycling and result in gradients on spatial scales ranging from millimeters to kilometers. Diverse and mostly uncharacterized microorganisms live in these habitats, and potentially play a role in mediating global scale biogeochemical processes. Quantifying the rates at which microbial activity in the subsurface occurs is a challenging endeavor, yet developing an understanding of these rates is essential to determine the impact of subsurface life on Earth's global biogeochemical cycles, and for understanding how microorganisms in these “extreme” environments survive (or even thrive). Here, we synthesize recent advances and discoveries pertaining to microbial activity in the marine deep subsurface, and we highlight topics about which there is still little understanding and suggest potential paths forward to address them. This publication is the result of a workshop held in August 2012 by the NSF-funded Center for Dark Energy Biosphere Investigations (C-DEBI) “theme team” on microbial activity (www.darkenergybiosphere.org).

show abstract

A precision multi-sampler for deep-sea hydrothermal microbial mat studies

Cited by 34 publications

References 27 publications

Subseafloor microbial communities in hydrogen‐rich vent fluids from hydrothermal systems along the Mid‐Cayman Rise

Subseafloor microbial communities in hydrogen‐rich vent fluids from hydrothermal systems along the Mid‐Cayman Rise

Bringing microbial diversity into focus: high‐resolution analysis of iron mats from the Lō‘ihi Seamount

Microbial activity in the marine deep biosphere: progress and prospects

Contact Info

Product

Resources

About