A prebiotic fructo-oligosaccharide promotes tight junction assembly in intestinal epithelial cells via an AMPK-dependent pathway

Wongkrasant, Preedajit; Pongkorpsakol, Pawin; Ariyadamrongkwan, Jutharat; Meesomboon, Roojanaat; Satitsri, Saravut; Pichyangkura, Rath; Barrett, Kim E.; Muanprasat, Chatchai

doi:10.1016/j.biopha.2020.110415

Cited by 40 publications

(31 citation statements)

References 54 publications

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“…In vitro supplementation with inulin fermentation products results in the significant upregulation of TJ genes including occludin, claudin-3 and ZO-1 [102]. Supplementation with fructooligosaccharides and butyrate, which is a bacterial metabolite produced during prebiotic fermentation, results in the redistribution of select proteins, including ZO-1 and occludin, to the vicinity of the TJs [103,104]. In vivo and in vivo studies demonstrated that galactooligosaccharide pretreatment results in upregulation of ZO-1, occludin and claudin-1 gene expression in LPS-challenged mice [105,106].…”

Section: Prebiotics Promote Barrier Function Through Microbial-dependent and -Independent Mechanismsmentioning

confidence: 99%

“…Given that prebiotics stimulate the growth of select probiotic species, it is unsurprising that prebiotic supplementation mirrors the beneficial effects produced by the same probiotics that those carbohydrates fuel. Furthermore, prebiotic supplementation results in robust activation of AMP-activated protein kinase (AMPK) in conjunction with the described changes to the epithelial barrier [98,103,104,106]. Prebiotic-mediated activation of AMPK may therefore play a significant role in prebiotics', and potentially probiotics', effects on intestinal tight junctions.…”

Section: Prebiotics Promote Barrier Function Through Microbial-dependent and -Independent Mechanismsmentioning

confidence: 99%

“…Prebiotic-mediated activation of AMPK may therefore play a significant role in prebiotics', and potentially probiotics', effects on intestinal tight junctions. Contrarily, data from two of the aforementioned studies suggest that some of prebiotics' beneficial effects may occur independently of the surrounding microbiota [96,104]. Although these microbial-independent effects are less robust than those produced with concurrent probiotic supplementation, the remarkable possibility of prebiotic-mediated effects despite concurrent dysbiosis, is undoubtedly worth pursing further.…”

Section: Prebiotics Promote Barrier Function Through Microbial-dependent and -Independent Mechanismsmentioning

confidence: 99%

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Probiotics, Prebiotics and Epithelial Tight Junctions: A Promising Approach to Modulate Intestinal Barrier Function

Rose

Odle

Blikslager

et al. 2021

IJMS

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Disruptions in the intestinal epithelial barrier can result in devastating consequences and a multitude of disease syndromes, particularly among preterm neonates. The association between barrier dysfunction and intestinal dysbiosis suggests that the intestinal barrier function is interactive with specific gut commensals and pathogenic microbes. In vitro and in vivo studies demonstrate that probiotic supplementation promotes significant upregulation and relocalization of interepithelial tight junction proteins, which form the microscopic scaffolds of the intestinal barrier. Probiotics facilitate some of these effects through the ligand-mediated stimulation of several toll-like receptors that are expressed by the intestinal epithelium. In particular, bacterial-mediated stimulation of toll-like receptor-2 modulates the expression and localization of specific protein constituents of intestinal tight junctions. Given that ingested prebiotics are robust modulators of the intestinal microbiota, prebiotic supplementation has been similarly investigated as a potential, indirect mechanism of barrier preservation. Emerging evidence suggests that prebiotics may additionally exert a direct effect on intestinal barrier function through mechanisms independent of the gut microbiota. In this review, we summarize current views on the effects of pro- and prebiotics on the intestinal epithelial barrier as well as on non-epithelial cell barrier constituents, such as the enteric glial cell network. Through continued investigation of these bioactive compounds, we can maximize their therapeutic potential for preventing and treating gastrointestinal diseases associated with impaired intestinal barrier function and dysbiosis.

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Section: Prebiotics Promote Barrier Function Through Microbial-dependent and -Independent Mechanismsmentioning

confidence: 99%

Section: Prebiotics Promote Barrier Function Through Microbial-dependent and -Independent Mechanismsmentioning

confidence: 99%

Section: Prebiotics Promote Barrier Function Through Microbial-dependent and -Independent Mechanismsmentioning

confidence: 99%

See 1 more Smart Citation

Probiotics, Prebiotics and Epithelial Tight Junctions: A Promising Approach to Modulate Intestinal Barrier Function

Rose

Odle

Blikslager

et al. 2021

IJMS

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“…In an in vitro study, a significant upregulation of tight junction genes including occludin, claudin-3, and ZO-1 was observed with the supplementation of inulin fermentation products to porcine intestinal epithelial cells [ 114 ]. The supplementation with fructo-oligosaccharides and butyrate to T84 human colonic epithelial cells and Caco-2 cells, respectively, resulted in the redistribution of proteins, including ZO-1 and occludin, to the vicinity of the tight junctions [ 115 , 116 ]. A reasonable explanation for these observed changes in tight junction protein expression and distribution is due to the direct effects of prebiotic on gut microbiota.…”

Section: Nutritional Intervention and Mechanistic Aspectsmentioning

confidence: 99%

The Impact of Weaning Stress on Gut Health and the Mechanistic Aspects of Several Feed Additives Contributing to Improved Gut Health Function in Weanling Piglets—A Review

Upadhaya

Kim

2021

Animals

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Newly weaned pig encounters psychosocial, physical, and nutritional stressors simultaneously when their immune system is not fully developed. These stressors have a cumulative effect on the immune response that contributes to the post-weaning growth lag which is characterized by depression in feed intake, reduced or negative growth rates, and increased susceptibility to pathogens in the first 24 to 48 h post-weaning. Consequently, the intestinal integrity, and digestive and absorptive capacity are impaired, and there is an increase in intestinal oxidative stress. It also causes the shifts in the taxonomic and functional properties of intestinal microbiome abruptly, thereby adversely affecting the health and performance of animals. It has been suggested that the effects of weaning stress on immune functions, intestinal barrier functions, and nervous system function in early weaned pigs extends into adulthood. The inclusion of different types of feed additives into the diet have been reported to alleviate the negative effects of weaning stress. The objective of this paper was to provide an overview on how the weaning stress affects gut health and the impact it has on production efficiencies, as well as the mechanistic aspects of several feed additives applied in reducing the weaning associated gut health problems and performance inefficiencies.

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“…For instance, mannan oligosaccharide (10 µM) promoted intestinal barrier function in T84 cells via AMPK activation [127,128]. Fructo-oligosaccharide (0.1 mg/mL), a prebiotic, accelerated intestinal tight junction reassembly via AMPK activation in T84 cells [129].…”

Section: Gastrointestinal Diseases and Gut Microbiotamentioning

confidence: 99%

Chitin and Chitosan Derivatives as Biomaterial Resources for Biological and Biomedical Applications

Satitsri

Muanprasat

2020

Molecules

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Chitin is a long-chain polymer of N-acetyl-glucosamine, which is regularly found in the exoskeleton of arthropods including insects, shellfish and the cell wall of fungi. It has been known that chitin can be used for biological and biomedical applications, especially as a biomaterial for tissue repairing, encapsulating drug for drug delivery. However, chitin has been postulated as an inducer of proinflammatory cytokines and certain diseases including asthma. Likewise, chitosan, a long-chain polymer of N-acetyl-glucosamine and d-glucosamine derived from chitin deacetylation, and chitosan oligosaccharide, a short chain polymer, have been known for their potential therapeutic effects, including anti-inflammatory, antioxidant, antidiarrheal, and anti-Alzheimer effects. This review summarizes potential utilization and limitation of chitin, chitosan and chitosan oligosaccharide in a variety of diseases. Furthermore, future direction of research and development of chitin, chitosan, and chitosan oligosaccharide for biomedical applications is discussed.

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A prebiotic fructo-oligosaccharide promotes tight junction assembly in intestinal epithelial cells via an AMPK-dependent pathway

Cited by 40 publications

References 54 publications

Probiotics, Prebiotics and Epithelial Tight Junctions: A Promising Approach to Modulate Intestinal Barrier Function

Probiotics, Prebiotics and Epithelial Tight Junctions: A Promising Approach to Modulate Intestinal Barrier Function

The Impact of Weaning Stress on Gut Health and the Mechanistic Aspects of Several Feed Additives Contributing to Improved Gut Health Function in Weanling Piglets—A Review

Chitin and Chitosan Derivatives as Biomaterial Resources for Biological and Biomedical Applications

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