A Practical Technique for Electrophysiologically Recording from Lamellated Antenna of Scarab Beetle

Chen, Li; Li, Ya-Ya; Shao, Kai-Min

doi:10.1007/s10886-019-01059-3

Cited by 11 publications

(17 citation statements)

References 48 publications

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“…Volatile collections were performed from 7:00 p.m. to 9:00 p.m. in June 2019, when the adults were active in the field. The aerial portion of the potted healthy peanut (Arachis hypogaea L.) plants was enclosed in a polyethylene (PE) bag (35 × 45 cm; Glad, Clorox China Limited, Guangzhou, China) and sealed tightly with cotton string [14]. Charcoal filtered continuous airflow at 550 mL/min was pumped into the bag using a vacuum pump (QC-1S; Beijing Municipal Institute of Labour Protection, Beijing, China) to maintain positive pressure.…”

Section: Headspace Plant Volatile Collectionmentioning

confidence: 99%

“…10 mL/min) at 150 • C for 30 min in an oven. Immediately after collection, the volatiles were eluted from the trap with 1 mL of dichloromethane [14]. Eight collections from different plants were carried out over a period of 2 h. The extracts were analyzed by gas chromatography (GC) and then combined and concentrated to 500 µL under a mild N 2 stream, and then kept at 4 • C for later use.…”

Section: Headspace Plant Volatile Collectionmentioning

confidence: 99%

“…GC-EAD recordings were carried out with a Shimadzu GC-2010plus equipped with a flame ionization detector (FID) and a DB-WAX capillary column (30 m × 0.25 mm × 0.25 µm, Agilent Technologies). The experimental details were described by Chen et al [14]. Briefly, injections (3 µL of sample, i.e., the dichloromethane solution of peanut plant volatiles eluted from the absorbent trap) onto the DB-WAX column were operated in a splitless mode with the split opened after 0.75 min.…”

Section: Gc and Gc-eadmentioning

confidence: 99%

“…The antenna of the beetle was excised and inserted between two glass capillaries (Outer Diameter 1.5 mm, Inter Diameter 0.84 mm, Vital Sense Scientific Instruments Co. Ltd., Chengdu, China) filled with Beadle-Ephrussi Ringer solution modified by Tween ® 80 (0.05%, W/V) [14]. The antenna base was inserted into the reference electrode, and one of the three lamellae was inserted into the recording electrode.…”

Section: Gc and Gc-eadmentioning

confidence: 99%

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Electrophysiological and Behavioral Responses of Holotrichia parallela to Volatiles from Peanut

Zhang

Cui

Xie

et al. 2021

Insects

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Holotrichia parallela (Coleoptera: Scarabaeidae: Melolonthinae) is a notorious pest of many crops, especially peanuts. In this study, volatiles from peanut plants were analyzed using both gas chromatographic-electroantennographic detection (GC-EAD) and gas chromatography/mass spectrometry (GC/MS) techniques, and tested for adult attraction with field trapping bioassays in Hebei Province, China. GC-EAD analyses indicated that H. parallela antennae strongly responded to twelve GC peaks, including eight identified compounds, (Z)-β-ocimene, hexanal, 6-methyl-5-hepten-2-one, nonanal, dihydromyrcenol, linalool, β-caryophyllene, methyl salicylate, and four unidentified compounds. When tested individually in field conditions from 24 to 31 July, 2020, β-caryophyllene and hexanal significantly attracted both sexes of H. parallela, whereas all other compounds were unattractive. A blend of β-caryophyllene and hexanal at a ratio of 2:1, close to the natural ratio of these two compounds from the intact peanut plant, was most attractive to the beetles. The remaining identified compounds, (Z)-β-ocimene, 6-methyl-5-hepten-2-one, nonanal, dihydromyrcenol, linalool, and methyl salicylate had no synergistic effects on H. parallela attraction when tested in combination with the blend of β-caryophyllene and hexanal. These results demonstrated that β-caryophyllene and hexanal in the volatiles from peanut plants have strong attraction to H. parallela. These two compounds have the potential to be used for monitoring H. parallela and its management programs.

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Section: Headspace Plant Volatile Collectionmentioning

confidence: 99%

Section: Headspace Plant Volatile Collectionmentioning

confidence: 99%

Section: Gc and Gc-eadmentioning

confidence: 99%

Section: Gc and Gc-eadmentioning

confidence: 99%

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Electrophysiological and Behavioral Responses of Holotrichia parallela to Volatiles from Peanut

Zhang

Cui

Xie

et al. 2021

Insects

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“…The antennal sensitivity of S. invicta workers to 2-ethyl-3,5-dimethylpyrazine was evaluated by conventional EAG methods as described in previous studies [18,24,26]. Briefly, an isolated ant head with antennae was connected between two glass electrodes filled with Ringer's solution modified by Tween ® 80 [27]. The indifferent electrode was inserted into the open side of the head while the recording electrode was brought into contact with the antennal tip.…”

Section: Antennal Sensitivity Recordingsmentioning

confidence: 99%

Electrophysiological and Alarm Responses of Solenopsis invicta Buren (Hymenoptera: Formicidae) to 2-Ethyl-3,5-dimethylpyrazine

Liu

Chen

2019

Insects

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2-Ethyl-3,5-dimethylpyrazine is an isomer of 2-ethyl-3,6-dimethylpyrazine, the alarm pheromone component of the red imported fire ant, Solenopsis invicta Buren. The pyrazine was synthesized and its alarm activity was investigated under laboratory conditions. It elicited significant electroantennogram (EAG) activities, and released characteristic alarm behaviors in fire ant workers. The EAG and alarm responses were both dose-dependent. Two doses of the pyrazine, 1 and 100 ng, were further subjected to bait discovery bioassays. Fire ant workers excited by the pyrazine were attracted to food baits, and their numbers increased over time. Ants displayed very similar response patterns to both low and high doses of the pyrazine. The pyrazine impregnated onto filter paper disc attracted significantly more fire ant workers than the hexane control for all observation time intervals at the low dose, and in the first 15 min period at the high dose. The pyrazine loaded onto food bait directly tended to attract more fire ant workers than the hexane control. These results support the potential use of 2-ethyl-3,5-dimethylpyrazine to enhance bait attractiveness for the control of S. invicta in invaded regions.

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Structure and distribution of antennal sensilla in Pseudosymmachia flavescens (Brenske) (Coleoptera: Scarabaeidae: Melolonthinae)

Shao

Liu

et al. 2021

Microscopy Res & Technique

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Morphology, microstructure, and distribution of antennal sensilla were compared between female and male Pseudosymmachia flavescens (Brenske) (Coleoptera: Scarabaeidae: Melolonthinae). Lamellate antennae of P. flavescens were shown to have typical scape, pedicel, and flagellum segments. The flagellum consists of a four‐segmented funicle and a three‐segmented club. The lengths of their pedicel and funicle were found to be similar in females and males. Distinct sexual differences were observed in the length of lamellar segments. Nine types/subtypes of sensilla were identified on the antennae of both sexes, including Böhm sensilla, sensilla trichodea, sensilla basiconica (SB), and two subtypes for sensilla chaetica, sensilla coeloconica, and sensilla placodea each. Olfactory sensilla (e.g., SB and placodea) are mainly located on three lamellar segments of the antennal club. Variation was also seen in abundance of various types of antennal sensilla, with males possessing significantly more sensilla than females. Sensilla placodea were the most abundant, and their number in males was twice of that in females, showing a clear sexual dimorphism. The difference in the distribution of sensilla placodea might reflect their roles in sexual chemical communication.

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A Practical Technique for Electrophysiologically Recording from Lamellated Antenna of Scarab Beetle

Cited by 11 publications

References 48 publications

Electrophysiological and Behavioral Responses of Holotrichia parallela to Volatiles from Peanut

Electrophysiological and Behavioral Responses of Holotrichia parallela to Volatiles from Peanut

Electrophysiological and Alarm Responses of Solenopsis invicta Buren (Hymenoptera: Formicidae) to 2-Ethyl-3,5-dimethylpyrazine

Structure and distribution of antennal sensilla in Pseudosymmachia flavescens (Brenske) (Coleoptera: Scarabaeidae: Melolonthinae)

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