A Practical Study of Procedures for the Detection of the Presence of Coliform Organisms in Water

A Comparison of MacConkey's Broth and Standard Lactose Broth as Media for Detection of Coliform Organisms in Water

“…It will be observed that, in the aggregate, the number of coliform isolations was about the same whether Mac- It is interesting to compare this experience with that encountered in an earlier study 7 of the relative utility of fuchsin broth and lactose broth primary media. The data requisite for such a comparison are shown in Table II.…”

“…; and as soon as gas appears in the tube, confirm completely as in (3) The brilliant green bile procedure was included because of the widespread interest in the favorable results secured by this means of confirmation in the course of a previous cooperative study. 7 The brilliant green bile (2 per cent) and eosin-methylene-blue (both in dehydrated form), the lactose, peptone, and neutral red employed were furnished through the courtesy of Difco Laboratories, Detroit, Mich. "Sodium Taurocholate" was purchased from …”

Failure of the most-probable-number technique to detect coliforms in drinking water and raw water supplies

“…The failure of coliforms to grow in BGLB when subcultured from lactose-containing media has been well documented (7,26,29,30) and was extremely prevalent when chlorinated potable water samples were examined (30). Even though the suitability of BGLB as a confirmatory medium has been demonstrated (19,21,28), BGLB may not be the best medium to use in the confirmatory test when chlorinated drinking water or untreated surface water supplies are examined.…”

Relative Value of 2 Per Cent and 5 Per Cent Brilliant Green Bile Confirmatory Media

“…Dunham, McCrady, and Jordan (3) used 5 per cent B.G.B. as a confirmatory medium and found that: (1) its use eliminated the large number of Endo plates on which growth did not occur; (2) spore-forming organisms were eliminated; and (3) the per cent of brilliant green cultures to lactose broth cultures was 99.5 per cent. With the use of filtered water, filtered water after chlorination, and tap water, they noticed that the B.G.B.…”