A practical review on the measurement tools for cellular adhesion force

Ungai-Salánki, Rita; Péter, Beatrix; Gerecsei, Tamás; Orgován, Norbert; Horváth, Róbert; Szabó, Bálint

doi:10.1016/j.cis.2019.05.005

Cited by 72 publications

(85 citation statements)

References 226 publications

(565 reference statements)

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“…Here, we studied the adhesive capacity of MDMs and MDDCs after LPS stimulation. We used both the classical, end-point adhesion assay and a computer-controlled micropipette method capable of measuring the force of adhesion [71]. When applying ligand binding site specific antibodies, we found that both anti-CD11b and anti-CD11c treatment weakened the adhesion to fibrinogen coated surfaces, as verified by the decreased number of adherent cells as well as the force of attachment.…”

Section: Plos Onementioning

confidence: 99%

The differential role of CR3 (CD11b/CD18) and CR4 (CD11c/CD18) in the adherence, migration and podosome formation of human macrophages and dendritic cells under inflammatory conditions

et al. 2020

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CR3 and CR4, the leukocyte specific β 2-integrins, involved in cellular adherence, migration and phagocytosis, are often assumed to have similar functions. Previously however, we proved that under physiological conditions CR4 is dominant in the adhesion to fibrinogen of human monocyte-derived macrophages (MDMs) and dendritic cells (MDDCs). Here, using inflammatory conditions, we provide further evidence that the expression and function of CR3 and CR4 are not identical in these cell types. We found that LPS treatment changes their expression differently on MDMs and MDDCs, suggesting a cell type specific regulation. Using mAb24, specific for the high affinity conformation of CD18, we proved that the activation and recycling of β 2-integrins is significantly enhanced upon LPS treatment. Adherence to fibrinogen was assessed by two fundamentally different approaches: a classical adhesion assay and a computer-controlled micropipette, capable of measuring adhesion strength. While both receptors participated in adhesion, we demonstrated that CR4 exerts a dominant role in the strong attachment of MDDCs. Studying the formation of podosomes we found that MDMs retain podosome formation after LPS activation, whereas MDDCs lose this ability, resulting in a significantly reduced adhesion force and an altered cellular distribution of CR3 and CR4. Our results suggest that inflammatory conditions reshape differentially the expression and role of CR3 and CR4 in macrophages and dendritic cells.

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Section: Plos Onementioning

confidence: 99%

The differential role of CR3 (CD11b/CD18) and CR4 (CD11c/CD18) in the adherence, migration and podosome formation of human macrophages and dendritic cells under inflammatory conditions

et al. 2020

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“…Over the years, numerous methods have been introduced to examine and quantify cell adhesion, from simple observations in an optical microscope to increasingly elaborate atomic force microscopy (AFM) techniques [15][16][17][18][19] . These techniques either measure cell-surface interactions and cell adhesion kinetics 10,[20][21][22][23][24] , or they are based on applying a force that can lead to cell detachment (termed adhesion strength measurements) on single-cells (e.g., micropipette aspiration, AFM, optical tweezer techniques) or on cell populations (e.g., centrifugation assay, spinning disk, flow chamber) 25 . One critical parameter of cell adhesion measurement methods is the throughput, describing the number of cells that can be detached in a certain period of time 26 .…”

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confidence: 99%

Single-cell adhesion force kinetics of cell populations from combined label-free optical biosensor and robotic fluidic force microscopy

Sztilkovics

Gerecsei

Péter

et al. 2020

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Single-cell adhesion force plays a crucial role in biological sciences, however its in-depth investigation is hindered by the extremely low throughput and the lack of temporal resolution of present techniques. While atomic force microcopy (AFM) based methods are capable of directly measuring the detachment force values between individual cells and a substrate, their throughput is limited to few cells per day, and cannot provide the kinetic evaluation of the adhesion force over the timescale of several hours. In this study a high spatial and temporal resolution resonant waveguide grating based label-free optical biosensor was combined with robotic fluidic force microscopy to monitor the adhesion of living cancer cells. In contrast to traditional fluidic force microscopy methods with a manipulation range in the order of 300-400 micrometers, the robotic device employed here can address single cells over mm-cm scale areas. This feature significantly increased measurement throughput, and opened the way to combine the technology with the employed microplate-based, large area biosensor. After calibrating the biosensor signals with the direct force measuring technology on 30 individual cells, the kinetic evaluation of the adhesion force and energy of large cell populations was performed for the first time. We concluded that the distribution of the single-cell adhesion force and energy can be fitted by lognormal functions as cells are spreading on the surface and revealed the dynamic changes in these distributions. The present methodology opens the way for the quantitative assessment of the kinetics of single-cell adhesion force and energy with an unprecedented throughput and time resolution, in a completely non-invasive manner.

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“…There are several ways to evaluate directly the cellular adhesion strength [ 84 ]. Bulk experiments are the most commonly used methods, which involve growing the cells on the substrate, exposing them to a buffer which produces cell detachment, and monitoring the percentage of cells which are capable of maintaining their adhesion to the surface.…”

Section: Tic Layers Obtained Using Pulsed Laser and Ion Plated Plamentioning

confidence: 99%

A Review of the Effect of a Nanostructured Thin Film Formed by Titanium Carbide and Titanium Oxides Clustered around Carbon in Graphitic Form on Osseointegration

2020

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Improving the biocompatibility of implants is an extremely important step towards improving their quality. In this review, we recount the technological and biological process for coating implants with thin films enriched in titanium carbide (TiC), which provide improved cell growth and osseointegration. At first, we discuss the use of a Pulsed Laser Ablation Deposition, which produced films with a good biocompatibility, cellular stimulation and osseointegration. We then describe how Ion Plating Plasma Assisted technology could be used to produce a nanostructured layer composed by graphitic carbon, whose biocompatibility is enhanced by titanium oxides and titanium carbide. In both cases, the nanostructured coating was compact and strongly bound to the bulk titanium, thus particularly useful to protect implants from the harsh oxidizing environment of biological tissues. The morphology and chemistry of the nanostructured coating were particularly desirable for osteoblasts, resulting in improved proliferation and differentiation. The cellular adhesion to the TiC-coated substrates was much stronger than to uncoated surfaces, and the number of philopodia and lamellipodia developed by the cells grown on the TiC-coated samples was higher. Finally, tests performed on rabbits confirmed in vivo that the osseointegration process of the TiC-coated implants is more efficient than that of uncoated titanium implants.

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A practical review on the measurement tools for cellular adhesion force

Cited by 72 publications

References 226 publications

The differential role of CR3 (CD11b/CD18) and CR4 (CD11c/CD18) in the adherence, migration and podosome formation of human macrophages and dendritic cells under inflammatory conditions

The differential role of CR3 (CD11b/CD18) and CR4 (CD11c/CD18) in the adherence, migration and podosome formation of human macrophages and dendritic cells under inflammatory conditions

Single-cell adhesion force kinetics of cell populations from combined label-free optical biosensor and robotic fluidic force microscopy

A Review of the Effect of a Nanostructured Thin Film Formed by Titanium Carbide and Titanium Oxides Clustered around Carbon in Graphitic Form on Osseointegration

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