A practical molecular clip for immobilization of receptors and biomolecules on devices’ surface: Synthesis, grafting protocol and analytical assay

Effects of immobilizing sites of RGD peptides in amphiphilic block copolymers on efficacy of cell adhesion

“…For the ABA-type of block copolymer, the PEG block is in the center of molecular chain and has no appropriate side group for direct coupling, so peptides are usually coupled just on the hydrophobic ends [60]. Recently, Marchand's group suggested to graft peptides to PEG-containing block copolymers [64,65] by an insertion reaction of azido-containing linkers [66]. In the present Fig.…”

Surface functionalization of germanium ATR devices for use in FTIR-biosensors

“…The molecular clip 5 (O-succinimidyl 4-(p-azidophenyl)butanoate) was prepared according to reference [15]. PEG molecules 1a and 1b (2-[methoxy(polyethyleneoxy)propyl]trimethoxysilane and trichlorosilane, 90%) were purchased from ABCR (Germany).…”

“…The nature of the organic layer being defined, we have to further choose the method of receptor anchorage for the biosensor device construction. We have previously disclosed a general strategy of (organic) surfaces functionalization based on the photografting of a so-called "molecular clip" [15], i.e. an arylazide derivative.…”

Impedimetric immunosensor based on humidity sensing properties of BST composite film

“…The polymer modified BST composite film can catch and bind to bimolecular species covalently through the carbonyl group of NHSMA. The carbonyl group in NHSMA could bind to biomolecules via nucleophilic displacement [82][83][84][85][86]. To immobilize the Bovine Serum Albumin (BSA) molecules on the surface, polymer modified samples were soaked in BSA solution for 16 hours at 4 °C.…”

“…trimethoxylsilyl)propyl methacrylate (TMS) and N-hydroxysuccinimide methacrylate (NHSMA) was synthesized using the Atom Transfer Radical Polymerization (ATRP) technique[74][75][76][77]. These copolymers consist of two segments: the segments of TMS are end-capped with trimethoxysilane moieties and can facilitate the cross-linking between the functionalized polymer and inorganic oxide network[78][79][80][81], while, NHSMA segments are capable of tethering of biomolecular species through aminolysis reaction[82][83][84][85][86].…”