A practical method for preparing peroxynitrite solutions of low ionic strength and free of hydrogen peroxide

Pryor, William A.; Cueto, Rafael; Jin, Xia; Koppenol, Willem H.; Ngu-Schwemlein, Maria; Squadrito, G; Uppu, Prasanna L.; Uppu, Rao M.

doi:10.1016/0891-5849(94)00105-s

Cited by 218 publications

(161 citation statements)

References 57 publications

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“…Aqueous stock solutions of NO, ONOO − and HbO # were prepared as described [28,38]. The O # − d -generating system consisted of 250 µM hypoxanthine and up to 100 m-units\ml xanthine oxidase resulting in, for example, an O # − d flux at 0.75 munits\ml xanthine oxidase of 0.5 µM\min.…”

Section: No Onoo − Hbo 2 H 2 O 2 and O 2 − D -Generating System mentioning

confidence: 99%

Autoinhibition of neuronal nitric oxide synthase: distinct effects of reactive nitrogen and oxygen species on enzyme activity

Kotsonis

Frey

Fröhlich

et al. 1999

Biochemical Journal

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Nitric oxide (NO) synthases (NOSs), which catalyse the oxidation of -arginine to -citrulline and an oxide of nitrogen, possibly NO or nitroxyl (NO − ), are subject to autoinhibition by a mechanism that has yet to be fully elucidated. In the present study we investigated the actions of NO and other NOS-derived products as possible autoregulators of enzyme activity. With the use of purified NOS-I, -arginine turnover was found to operate initially at V max (0-15 min, phase I) although, despite the presence of excess substrate and cofactors, prolonged catalysis (15-90 min, phase II) was associated with a rapid decline in -arginine turnover. Taken together, these observations suggested that one or more NOS products inactivate NOS. Indeed, exogenously applied reactive nitrogen oxide species (RNSs) decreased V max during phase I, although with different potencies (NO − NO ONOO − ) and efficacies (NO NO − l ONOO − ). The NO scavengers oxyhaemoglobin (HbO # ; 100 µM) and 1H-imidazol-1-yloxy-2-(4-carboxyphenyl)-4,5-dihydro-4,4,5,5-tetramethyl-3-

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Section: No Onoo − Hbo 2 H 2 O 2 and O 2 − D -Generating System mentioning

confidence: 99%

Autoinhibition of neuronal nitric oxide synthase: distinct effects of reactive nitrogen and oxygen species on enzyme activity

Kotsonis

Frey

Fröhlich

et al. 1999

Biochemical Journal

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“…SSDI 0014-5793(95)01530-2 complex sodium salt (DEA/NO; Research Biochemicals International), essentially as described by Maragos et al [18]. Peroxynitrite was synthesized by ozonization of ice-cooled aqueous sodium azide at pH 12, essentially as described [19] (precautions for the procedure are described in ref. [19]).…”

Section: Chemicalsmentioning

confidence: 99%

“…Peroxynitrite was synthesized by ozonization of ice-cooled aqueous sodium azide at pH 12, essentially as described [19] (precautions for the procedure are described in ref. [19]). The actual peroxynitrite concentrations were determined before each experiment at "~max = 302 nm taking es02 = 1,670 M -I "cm -1 [20].…”

Section: Chemicalsmentioning

confidence: 99%

Accumulation of nitrotyrosine on the SERCA2a isoform of SR Ca‐ATPase of rat skeletal muscle during aging: a peroxynitrite‐mediated process?

Viner¹,

Ferrington²,

Hühmer³

et al. 1996

FEBS Letters

131

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The SR Ca-ATPase in skeletal muscle SR vesicles isolated from young adult (5 months) and aged (28 months) rats was analyzed for nitrotyrosine. Only the SERCA2a isoform contained significant amounts with approximately one and four nitrotyrosine residues per young and old Ca-ATPase, respectively. The in vitro exposure of SR vesicles of young rats to peroxynitrite yielded selective nitration of the SERCA2a Ca-ATPase even in the presence of excess SERCAla. No nitration was observed during the exposure of SR vesicles to nitric oxide in the presence of 02. These data suggest the in vivo presence of peroxynitrite in skeletal muscle. The greater nitrotyrosine content of SERCA2a from aged tissue implies an age-associated increase in susceptibility to oxidation by this species.

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“…PMSF was from Nacalai Tesque (Kyoto, Japan). 3-Morpholinosydnonimine (SIN-1) was from Dojindo (Kumamoto, Japan 23,25) The solution was stored at Ϫ80°C until use. The concentration of the peroxynitrite solution was determined spectrophotometrically by measuring the absorbance at 302 nm (eϭ1670 M Ϫ1 cm…”

Section: Methodsmentioning

confidence: 99%

Nitration of Specific Tyrosine Residues of Cytochrome c Is Associated with Caspase-Cascade Inactivation

Nakagawa

Komai

Takusagawa

et al. 2007

Biological & Pharmaceutical Bulletin

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A practical method for preparing peroxynitrite solutions of low ionic strength and free of hydrogen peroxide

Cited by 218 publications

References 57 publications

Autoinhibition of neuronal nitric oxide synthase: distinct effects of reactive nitrogen and oxygen species on enzyme activity

Autoinhibition of neuronal nitric oxide synthase: distinct effects of reactive nitrogen and oxygen species on enzyme activity

Accumulation of nitrotyrosine on the SERCA2a isoform of SR Ca‐ATPase of rat skeletal muscle during aging: a peroxynitrite‐mediated process?

Nitration of Specific Tyrosine Residues of Cytochrome c Is Associated with Caspase-Cascade Inactivation

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