A Practical Manual of Medical and Biological Staining Techniques

Gurr, Edward

doi:10.2307/1292057

Cited by 35 publications

(6 citation statements)

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“…Ichthyol. 27 (2011), 67-72 Ó 2010 Ehrlich hematoxylin, and counterstained them with eosin to determine their general histology (Gurr, 1956). To determine the general morphology of the spines, alcian blue-alizarin red S was used (Humanson, 1972).…”

Section: Methodsmentioning

confidence: 99%

Structure and development of spines over the skin surface of the river puffer Takifugu obscurus (Tetraodontidae, Teleostei) during larval growth

Byeon

Park

Yoon

et al. 2010

Journal of Applied Ichthyology

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The histological structure and development of spines on the skin surface of Takifugu obscurus were studied during larval development conducted artificially with an average 30& salinity and 18.0-20.3°C water temperature. The epidermis comprises an outermost layer, middle layer, and the stratum germinativum, and contains three types of gland cells: small spherical or flask-shaped mucous cells, larger sacciform mucous cells, and large granular cells. The dermis and subcutis follow. The spines first appear over the ventral region at 10 days after hatching and consist of two parts: a central long tapering portion which projects into the epidermis and eventually outside of the body, and a short supporting basal portion that is embedded within the stratum compactum layer of the dermis. The central, long tapering portion has two very short processes on top until 25 days after hatching, but these two separate spines fuse into one 30 days after hatching. In contrast, the short supporting spines rooted at the base consist of three to six small spines (usually four to five spines) and are present even in the adult stage. Therefore, calcareous spines consisting of one central long spine and three to six smaller supporting spines form tetra-and septaradiate spines (mainly penta-and hexaradiate). The spines first appear over the ventral region.

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Section: Methodsmentioning

confidence: 99%

Structure and development of spines over the skin surface of the river puffer Takifugu obscurus (Tetraodontidae, Teleostei) during larval growth

Byeon

Park

Yoon

et al. 2010

Journal of Applied Ichthyology

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“…The pancreas was fixed in 10 per cent formalin or Bouin's solution. Paraffin sections were cut at 4 to 5 ^ and stained with hematoxylin-eosin, Feulgen reagent, Gomori's chrome alum hematoxylin phloxine, 32 aldehyde fuchsin phloxine, Cason's modification of the Mallory Heidenhain stain, 33 or chrome hematoxylin aldehyde fuchsin.…”

Section: Methodsmentioning

confidence: 99%

Postnatal Neogenesis of Islets of Langerhans in the Mouse

Bunnag

1966

Diabetes

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The source of the new islets during growth and the effect of aging on the number of islets have been studied in Webster Swiss mice. A combination of methods was employed, including microscopy in vivo, enumeration of the total number of islets, radioautography with H-3-thymidine, and conventional histological technics. The total number of islets in normal mice increases with age until about six months, and then becomes stable or decreases slightly. Radioautographs showed that the cells of the islets are in all likelihood derived from migrating ductal or periductal cells residing at the junction between the islet and pancreatic duct and at the periphery of the islet, and that these cells doubtless serve as the progenitor pool for the growth of the islet. The nuclear cycle of the insular cells was also determined by this method. In vivo study revealed that the structural integrity of ducts and of their vascular supply is important for the maintenance of the population of ductal and periductal islets. DIABETES 75:480-91, July, 1966.Regeneration of pancreatic tissue and formation of new islets of Langerhans in adult mammals of various species have been described in a variety of experimental conditions, including partial or complete pancreatectomy, 1 " 7 ligation of the pancreatic duct, 8 ' 9 ligation of the pancreatic arteries, 10 prolonged hyperglycemia produced by intravenous infusion of glucose, 11 protracted glucagon administration, 12 force-feeding of a high carbohydrate diet 13 and the administration of alloxan. 14 " 16 Whether new islets of Langerhans are formed in the resting pancreas of adult mammals under normal conditions is disputed, 7 " 22 and information concerning the formation of islets in aging is scanty.In both the experimental and the normal state, the

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“…The adrenals were fixed in formalsublimate-acetic acid (FSA) [16] and in 10% neutral formalin. Paraffin sections cut at 4 lm were stained with haematoxylin and eosin and azan [17]. The alcian-blue periodic acid-Schiff reaction (AB/PAS) for glycoprotein [18] was also performed on paraffin-sectioned material.…”

Section: Adrenals Samplingmentioning

confidence: 99%