A practical guide to promote informatics-driven efficient biotopographic material development

Guo, Yuanlong; Mi, Jiaomei; Wang, Hongzhi; Ao, Yong; Shi, Mengru; Shan, Zhengjie; Li, Bingzhi; Chen, Zetao; Chen, Zhuofan; Vasilev, Krasimir; Xiao, Yin

doi:10.1016/j.bioactmat.2021.06.013

Cited by 3 publications

(2 citation statements)

References 41 publications

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“…Wound healing assay including lentivirus transduction of GFP labels into fibroblasts and flow-based cell sorting was as previously described [ 4 , 36 ]. To be specific, cells were seeded in each side of the box of the healing insert (Ibidi, Germany).…”

Section: Methodsmentioning

confidence: 99%

Improving hard metal implant and soft tissue integration by modulating the “inflammatory-fibrous complex” response

Huang

Xie

et al. 2023

Bioactive Materials

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Section: Methodsmentioning

confidence: 99%

Improving hard metal implant and soft tissue integration by modulating the “inflammatory-fibrous complex” response

Huang

Xie

et al. 2023

Bioactive Materials

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“…The biomechanical force generated by high-aspect-ratio nanowire arrays was discovered to be in the nanonewton scale, which may modulate other mechanosensitive membrane proteins, rather than integrin β 2 [23]. For example, TiO 2 nanotubes with high-aspect-ratio nanotopographic structures have been shown to modulate the mechanosensitive ion channel Piezo1 [24]. However, integrin β 2 may experience a piconewton scale stretching force in the activation state, and the generated biomechanical forces that even penetrate the cell membrane by the high-aspect-ratio nanotopographic structures are too large to finely and flexibly tune its conformation extension degree.…”

Section: Introductionmentioning

confidence: 99%

Nanotopographic micro-nano forces finely tune the conformation of macrophage mechanosensitive membrane protein integrin β2 to manipulate inflammatory responses

Guo

Wang

et al. 2023

Nano Res.

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Finely tuning mechanosensitive membrane proteins holds great potential in precisely controlling inflammatory responses. In addition to macroscopic force, mechanosensitive membrane proteins are reported to be sensitive to micro-nano forces. Integrin β 2 , for example, might undergo a piconewton scale stretching force in the activation state. High-aspect-ratio nanotopographic structures were found to generate nN-scale biomechanical force. Together with the advantages of uniform and precisely tunable structural parameters, it is fascinating to develop low-aspect-ratio nanotopographic structures to generate micro-nano forces for finely modulating their conformations and the subsequent mechanoimmiune responses. In this study, low-aspect-ratio nanotopographic structures were developed to finely manipulate the conformation of integrin β 2 . The direct interaction of forces and the model molecule integrin α X β 2 was first performed. It was demonstrated that pressing force could successfully induce conformational compression and deactivation of integrin α X β 2 , and approximately 270 to 720 pN may be required to inhibit its conformational extension and activation. Three low-aspect-ratio nanotopographic surfaces (nanohemispheres, nanorods, and nanoholes) with various structural parameters were specially designed to generate the micro-nano forces. It was found that the nanorods and nanohemispheres surfaces induce greater contact pressure at the contact interface between macrophages and nanotopographic structures, particularly after cell adhesion. These higher contact pressures successfully inhibited the conformational extension and activation of integrin β 2 , suppressing focal adhesion activity and the downstream PI3K-Akt signaling pathway, reducing NF- κ B signaling and macrophage inflammatory responses. Our findings suggest that nanotopographic structures can be used to finely tune mechanosensitive membrane protein conformation changes, providing an effective strategy for precisely modulating inflammatory responses. Electronic Supplementary Material Supplementary material (primer sequences of target genes in RT-qPCR assay; the results of solvent accessible surface area during equilibrium simulation, the ligplut results of hydrogen bonds, and hydrophobic interactions; the density of different nanotopographic structures; interaction analysis of the downregulated leading genes of “focal adhesion” signaling pathway in nanohemispheres and nanorods groups; and the GSEA results of “Rap 1 signaling pathway” and “regulation of actin cytoskeleton” in different groups) is available in the online version of this article at 10.1007/s12274-023-5550-0.

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Silicon nanostructures and nanocomposites for antibacterial and theranostic applications

Kumar¹,

Devi²,

Kumar³

et al. 2022

Sensors and Actuators A: Physical

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A practical guide to promote informatics-driven efficient biotopographic material development

Cited by 3 publications

References 41 publications

Improving hard metal implant and soft tissue integration by modulating the “inflammatory-fibrous complex” response

Improving hard metal implant and soft tissue integration by modulating the “inflammatory-fibrous complex” response

Nanotopographic micro-nano forces finely tune the conformation of macrophage mechanosensitive membrane protein integrin β2 to manipulate inflammatory responses

Silicon nanostructures and nanocomposites for antibacterial and theranostic applications

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