A POU-domain transcription factor in early stem cells and germ cells of the mammalian embryo

Rosner, Mitchell H.; Viganò, M. Alessandra; Ozato, Keiko; Timmons, Paula M.; Poirier, Françoise; Rigby, P. W. J.; Staudt, Louis M.

doi:10.1038/345686a0

Cited by 866 publications

(676 citation statements)

References 41 publications

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“…Of interest, it has recently been shown that Trh may be a direct transcriptional target of POU5F1, a well-characterized marker of pluripotency in embryonic stem cells and the epiblast (Loh et al, 2006;Matoba et al, 2006). Pou5f1 expression in the epiblast overlaps with that of Trh, suggesting that POU5F1 may in part be regulating Trh expression at this stage (Rosner et al, 1990).…”

Section: Discussionmentioning

confidence: 99%

Dynamic expression of Thyrotropin‐releasing hormone in the mouse definitive endoderm

McKnight¹,

Hou²,

Hoodless³

2007

Developmental Dynamics

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Thyrotropin-releasing hormone (TRH) is a well-characterized regulator of the hypothalamic-pituitary-thyroid endocrine axis. Here, we describe the expression of Trh during early embryonic development in the mouse. We find Trh to be highly expressed during postimplantation stages in the mouse embryo, with expression first observed in the epiblast at embryonic day (E) 6.5. During gastrulation, Trh is expressed in the newly formed definitive endoderm cells, and at embryonic day (E) 7.75, marks the entire definitive endoderm. Subsequently, Trh mRNA levels rapidly decrease such that, by E9.0, expression in the definitive endoderm is no longer detected, after which neural expression predominates. Thus, Trh is expressed dynamically and specifically in the developing mouse definitive endoderm from E7.0 to E8.5. Trh is unique among definitive endoderm markers as it transiently marks the entire definitive endoderm population and is not expressed in the extraembryonic endoderm. Trh will be a valuable tool to study definitive endoderm formation in the mouse embryo.

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Section: Discussionmentioning

confidence: 99%

Dynamic expression of Thyrotropin‐releasing hormone in the mouse definitive endoderm

McKnight¹,

Hou²,

Hoodless³

2007

Developmental Dynamics

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“…20,[24][25][26] During this time, the pluripotency-associated genes Oct4, Sox2, and Nanog are progressively restricted to the germline and become germ cell specific at E8.0. [27][28][29] The means by which a limited subset of cells is initially selected from among the larger number of competent cells in the correct position of the posterior epiblast is unclear.…”

Section: Specification Of the Germlinementioning

confidence: 99%

“…31 Likewise, Oct4 is required to form Dppa3-expressing PGCs but is expressed in cells outside the putative germ cell lineage at the time of specification. 29,32 Despite the division between preformation and induction, fundamental similarities exist in the germ cell specification of worm, fly, and mouse. Each species sets aside a dedicated set of germ cell precursors -P 4 35 A maternally-inherited P granule protein, PIE-1, is required for this transcriptional silencing: in embryos derived from pie-1 mutants, zygotic transcripts are aberrantly present in the embryonic germ cell precursors, and the germline does not develop.…”

Section: Specification Of the Germlinementioning

confidence: 99%

Genetics of germ cell development

2012

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| The germ line represents a continuous cellular link between generations and between species, but the germ cells themselves develop in a specialized, organism-specific context. The three most common animal model organisms, C. elegans, Drosophila, and mouse, display striking similarities as well as major differences in the means by which they control germ cell development.Comparison of the germ cells of these three organisms sheds light not only on universal aspects of germline regulation, but also on control of the pluripotent state in vivo and on the earliest steps of embryogenesis. Taking a broad perspective, we will follow the germ cells from specification in the early embryo, through gametogenesis, to fertilization, and highlight themes from the comparison of three alternative strategies for navigating the fundamental cycle of sexual reproduction.

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“…Specific expression of Oct 3/4 has been related to the pluripotent capacity of testicular GCTs, suggesting their origin from transformed pluripotent progenitor cells, such as PGCs [100]. Oct 3/4 is critical for the self-renewal of ES cells [124] and, under physiological conditions, Oct 3/4 transcription factor is expressed exclusively in pluripotent ES cells, PGCs, oogonia, and gonocytes [60,65,147] and its expression is downregulated during differentiation [138]. Many similarities exist between testicular and ovarian GCTs, including morphological resemblance and a similar pattern of chromosomal alterations [143].…”

Section: Ovarian Cancer: Pluripotency and Putative Cscsmentioning

confidence: 99%

Cancer stem cells as targets for cancer therapy: selected cancers as examples

Hombach‐Klonisch

Paranjothy

Wiecheć

et al. 2008

Arch. Immunol. Ther. Exp.

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It is becoming increasingly evident that cancer constitutes a group of diseases involving altered stem-cell maturation/differentiation and the disturbance of regenerative processes. The observed malignant transformation is merely a symptom of normal differentiation processes gone astray rather than the primary event. This review focuses on the role of cancer stem cells (CSCs) in three common but also relatively under-investigated cancers: head and neck, ovarian, and testicular cancer. For didactic purpose, the physiology of stem cells is first introduced using hematopoietic and mesenchymal stem cells as examples. This is followed by a discussion of the (possible) role of CSCs in head and neck, ovarian, and testicular cancer. Aside from basic information about the pathophysiology of these cancers, current research results focused on the discovery of molecular markers specific to these cancers are also discussed. The last part of the review is largely dedicated to signaling pathways active within various normal and CSC types (e.g. Nanog, Nestin, Notch1, Notch2, Oct3 and 4, Wnt). Different elements of these pathways are also discussed in the context

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A POU-domain transcription factor in early stem cells and germ cells of the mammalian embryo

Cited by 866 publications

References 41 publications

Dynamic expression of Thyrotropin‐releasing hormone in the mouse definitive endoderm

Dynamic expression of Thyrotropin‐releasing hormone in the mouse definitive endoderm

Genetics of germ cell development

Cancer stem cells as targets for cancer therapy: selected cancers as examples

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