A potentiometric phosphate biosensor based on entrapment of pyruvate oxidase in a polypyrrole film

Ogabiela, Elaigwu Edward; Adeloju, Samuel B O

doi:10.1039/c3ay42172d

Cited by 15 publications

(4 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In rivers there is more accumulation of organic matter and microorganisms and the soil is impregnated with organic matter. For both types of water sample, the values are in very good agreement with those expected [40][41][42].…”

Section: Real Sample Analysissupporting

confidence: 83%

Pyruvate Oxidase Biosensors Based on Glassy Carbon Electrodes Modified with Carbon Nanotubes and Poly(Neutral Red) Synthesized in Ethaline Deep Eutectic Solvent

2021

View full text Add to dashboard Cite

A novel nanostructured platform for pyruvate oxidase biosensors comprises poly(neutral red) (PNR) prepared by electropolymerization of NR in ethaline deep eutectic solvent (DES) with acid dopant, on a multiwalled carbon nanotubes (MWCNT) modified glassy carbon electrode (GCE). Characterization was by cyclic voltammetry and electrochemical impedance spectroscopy and morphology was examined by scanning electron micro-scopy. Ascorbate and H 2 O 2 gave a better response at PNR DES /GCE than at PNR aq /GCE. Biosensors for pyruvate and phosphate, immobilizing pyruvate oxidase onto PNR DES /MWCNT/GCE enabled selective determination of pyruvate and phosphate, with micromolar limits of detection. Pyruvate was determined in onion samples and phosphate in water samples. Keywords: Ethaline deep eutectic solvent • poly(neutral red) • MWCNT • pyruvate oxidase • phosphate [a] R.

show abstract

Section: Real Sample Analysissupporting

confidence: 83%

Pyruvate Oxidase Biosensors Based on Glassy Carbon Electrodes Modified with Carbon Nanotubes and Poly(Neutral Red) Synthesized in Ethaline Deep Eutectic Solvent

2021

View full text Add to dashboard Cite

show abstract

“…In potentiometric phosphate biosensors studies, it is stated that H + produced by the electrooxidation of H 2 O 2 leads to potential changes and these changes are associated with phosphate ion concentration . However, as soon as phosphate is added to the reaction medium, potential shift may occur due to the change of the anion balance.…”

Section: Resultsmentioning

confidence: 99%

“…Conventional and instrumental analytical techniques such as gravimetric, volumetric, chromatographic and spectrophotometric measurements are used for determination of phosphate concentration [5]. These methods have disadvantages such as producing toxic waste, exhausting, time-consuming, requiring expensive instrumentation and expertise [5,6]. In addition, these techniques including carcinogenic chemicals such as molybdenum makes health hazardous [2].…”

Section: Introductionmentioning

confidence: 99%

Poly(pyrrole‐co‐pyrrole‐2‐carboxylic acid)/Pyruvate Oxidase Based Biosensor for Phosphate: Determination of the Potential, and Application in Streams

2019

View full text Add to dashboard Cite

A biosensor based on conductive poly(pyrrole‐co‐pyrrole‐2‐carboxylic acid) [Poly(Py‐co‐PyCOOH)] copolymer film coated gold electrode was developed for the quantitative phosphate determination. Enzyme pyruvate oxidase was immobilized chemically via the functional carboxylated groups of the copolymer. The potential to be applied which is deficiency of phosphate biosensor studies for precise phosphate detection was clarified by using differential pulse voltammetry technique. Performance of the sensing ability of the biosensor was improved by optimizing cofactor/cosubstrate concentrations, polymeric film density and pH. The biosensor showed a linearity up to phosphate concentration of 5 mM, operational stability with a relative standard deviation (RSD) of 0.07 % (n=7) and accuracy of 101 % at −0.15 V (vs. Ag/AgCl). Detection limit (LOD) and sensitivity were calculated to be 13.3 μM and 5.4 μA mM−1 cm−2, respectively by preserving 50 % of its initial response at the end of 30 days. It's performance was tested to determine phosphate concentrations in two streams of Zonguldak City in Turkey. Accuracy of phosphate measurement in stream water was found to be 91 %.

show abstract

“…As a conducting polymer, PPy being wealthy in amino groups has been extensively investigated for enzyme immobilization [32], DNA extraction [33], and drug delivery [34]. All the investigations confirm excellent biocompatibility of PPy.…”

Section: Introductionmentioning

confidence: 97%

Conjugation of methotrexate onto dedoped Fe3O4/PPy nanospheres to produce magnetic targeting drug with controlled drug release and targeting specificity for HeLa cells

et al. 2015

View full text Add to dashboard Cite

A potentiometric phosphate biosensor based on entrapment of pyruvate oxidase in a polypyrrole film

Abstract: A polypyrrole based potentiometric phosphate biosensor has been fabricated by entrapment of pyruvate oxidase in a polypyrrole film.

Cited by 15 publications

References 54 publications

Pyruvate Oxidase Biosensors Based on Glassy Carbon Electrodes Modified with Carbon Nanotubes and Poly(Neutral Red) Synthesized in Ethaline Deep Eutectic Solvent

Pyruvate Oxidase Biosensors Based on Glassy Carbon Electrodes Modified with Carbon Nanotubes and Poly(Neutral Red) Synthesized in Ethaline Deep Eutectic Solvent

Poly(pyrrole‐co‐pyrrole‐2‐carboxylic acid)/Pyruvate Oxidase Based Biosensor for Phosphate: Determination of the Potential, and Application in Streams

Conjugation of methotrexate onto dedoped Fe3O4/PPy nanospheres to produce magnetic targeting drug with controlled drug release and targeting specificity for HeLa cells

Contact Info

Product

Resources

About