A potential role of chondroitin sulfate on bone in osteoarthritis: inhibition of prostaglandin E2 and matrix metalloproteinases synthesis in interleukin-1β- stimulated osteoblasts

Pecchi, Émilie; Priam, Sabrina; Mladenović, Z.; Gosset, Marjolaine; Saurel, Anne-Sophie; Aguilar, L.; Bérenbaum, Francis; Jacques, Claire

doi:10.1016/j.joca.2011.12.002

Cited by 53 publications

(31 citation statements)

References 52 publications

(61 reference statements)

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“…22,25 The study of extracellular components, such as proteoglycans, fibrous proteins, glycoproteins, metalloproteases, and glycosidases, can shed light on the molecular changes directly related to the development of such diseases. 16,24,26 …”

Section: Discussionmentioning

confidence: 99%

Expression of heparanase in basal cell carcinoma and squamous cell carcinoma

Pinhal

Almeida

Costa

et al. 2016

An. Bras. Dermatol.

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BackgroundHeparanase is an enzyme that cleaves heparan sulfate chains. Oligosaccharides generated by heparanase induce tumor progression. Basal cell carcinoma and squamous cell carcinoma comprise types of nonmelanoma skin cancer.ObjectivesEvaluate the glycosaminoglycans profile and expression of heparanase in two human cell lines established in culture, immortalized skin keratinocyte (HaCaT) and squamous cell carcinoma (A431) and also investigate the expression of heparanase in basal cell carcinoma, squamous cell carcinoma and eyelid skin of individuals not affected by the disease (control).MethodsGlycosaminoglycans were quantified by electrophoresis and indirect ELISA method. The heparanase expression was analyzed by quantitative RT-PCR (qRTPCR).ResultsThe A431 strain showed significant increase in the sulfated glycosaminoglycans, increased heparanase expression and decreased hyaluronic acid, comparing to the HaCaT lineage. The mRNA expression of heparanase was significantly higher in Basal cell carcinoma and squamous cell carcinoma compared with control skin samples. It was also observed increased heparanase expression in squamous cell carcinoma compared to the Basal cell carcinoma.ConclusionThe glycosaminoglycans profile, as well as heparanase expression are different between HaCaT and A431 cell lines. The increased expression of heparanase in Basal cell carcinoma and squamous cell carcinoma suggests that this enzyme could be a marker for the diagnosis of such types of non-melanoma cancers, and may be useful as a target molecule for future alternative treatment.

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Section: Discussionmentioning

confidence: 99%

Expression of heparanase in basal cell carcinoma and squamous cell carcinoma

Pinhal

Almeida

Costa

et al. 2016

An. Bras. Dermatol.

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“…For instance, Pecchi et al. found that the treatment of IL‐1β inhibited osteoblast maturation by up‐regulating the expression of cyclooxygenase‐2 and metalloproteinase to degrade the extracellular matrix of bone tissue. In contrast, short‐term exposure of IL‐1β promoted nodule formation , and the injection of IL‐1β accelerated endochondral ossification in mice bone fracture .…”

Section: Discussionmentioning

confidence: 99%

Melatonin mediates protective effects on inflammatory response induced by interleukin‐1 beta in human mesenchymal stem cells

Liu

Gong

Xiong

et al. 2013

Journal of Pineal Research

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Joint diseases like osteoarthritis usually are accompanied with inflammatory processes, in which pro-inflammatory cytokines mediate the generation of intracellular reactive oxygen species (ROS) and compromise survival of subchondral osteoblasts. Melatonin is capable of manipulating bone formation and osteogenic differentiation of mesenchymal stem cells (MSCs). The aim of this work was to investigate the anti-inflammatory effect of melatonin on MSC proliferation and osteogenic differentiation in the absence or presence of interleukin-1 beta (IL-1β), which was used to induce inflammation. Our data showed that melatonin improved cell viability and reduced ROS generation in MSCs in a dose-dependent manner. When exposed to 10 ng/mL IL-1β, various concentrations of melatonin resulted in significant reduction of ROS by 34.9% averagely. Luzindole as a melatonin receptor antagonist reversed the anti-oxidant effect of melatonin in MSCs with co-exposure to IL-1β. Real-time RT-PCR data suggested that melatonin treatment up-regulated the expression of CuZnSOD and MnSOD, while down-regulated the expression of Bax. To investigate the effect of melatonin on osteogenesis, MSCs were cultured in osteogenic differentiation medium supplemented with IL-1β, melatonin, or luzindole. After exposed to IL-1β for 21 days, 1 μm melatonin treatment significantly increased the levels of type I collagen, ALP, and osteocalcin, and 100 μm melatonin treatment yielded the highest level of osteopontin. Our study demonstrated that melatonin maintained MSC survival and promoted osteogenic differentiation in inflammatory environment induced by IL-1β, suggesting melatonin treatment could be a promising method for bone regenerative engineering in future studies.

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“…A similar phenomenon has been observed when using porcine gastrointestinal tissues, which required pre-treatment with a cyclooxygenase inhibitor to study subsequent prostaglandin-dependent outcome measures 23 . The porcine and murine IL-1β cDNA sequence show 74% homologies with the human form 24 and use of human recombinant IL-1β has been effective in tissues from both species 25 - 30 . Nevertheless, heterology between human and porcine cytokines should also be considered in the absence of a significant IL-1β effect.…”

Section: Discussionmentioning

confidence: 55%

Ex vivo effect of gold nanoparticles on porcine synovial membrane

et al. 2013

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Gold nanoparticles (AuNPs) have great potential as carriers for local drug delivery and as a primary therapeutic for treatment of inflammation. Here we report on the AuNP-synovium interaction in an ex vivo model of intra-articular application for treatment of joint inflammation. Sheets of porcine femoropatellar synovium were obtained post mortem and each side of the tissue samples was maintained in a separate fluid environment. Permeability to AuNPs of different sizes (5−52 nm) and biomarker levels of inflammation were determined to characterize the ex vivo particle interaction with the synovium. Lipopolysaccharide or recombinant human interleukin-1β were added to fluid environments to assess the ex vivo effect of pro-inflammatory factors on permeability and biomarker levels. The synovium showed size selective permeability with only 5 nm AuNPs effectively permeating the entire tissues’ width. This process was further governed by particle stability in the fluid environment. AuNPs reduced matrix metalloproteinase and lactate dehydrogenase activity and hyaluronic acid concentrations but had no effect on prostaglandin E2 levels. Exposure to pro-inflammatory factors did not significantly affect AuNP permeation or biomarker levels in this model. Results with ex vivo tissue modeling of porcine synovium support an anti-inflammatory effect of AuNPs warranting further investigation.

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A potential role of chondroitin sulfate on bone in osteoarthritis: inhibition of prostaglandin E2 and matrix metalloproteinases synthesis in interleukin-1β- stimulated osteoblasts

Cited by 53 publications

References 52 publications

Expression of heparanase in basal cell carcinoma and squamous cell carcinoma

Expression of heparanase in basal cell carcinoma and squamous cell carcinoma

Melatonin mediates protective effects on inflammatory response induced by interleukin‐1 beta in human mesenchymal stem cells

Ex vivo effect of gold nanoparticles on porcine synovial membrane

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