A potential role of aminoglycoside resistance in endemic occurrence of Pseudomonas aeruginosa strains in lower airways of mechanically ventilated patients

Mózes, Julianna; Szűcs, István; Molnár, Dávid; Jakab, Péter; Ebrahimi, Fatemeh; Szilasi, Mária; Majoros, László; Orosi, Piroska; Kardos, Gábor

doi:10.1016/j.diagmicrobio.2013.09.015

Cited by 8 publications

(4 citation statements)

References 29 publications

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“…The population structure of P. aeruginosa is reported as multiclonal in most studies [ 27 , 28 ]; even when major clones were present, the population was characterized by high diversity and by the presence of several different clones. Similarly, multiple versatile P. aeruginosa clones may be circulating in our hospital, as reported in a previous study with a more limited scope conducted in a ward of the same hospital [ 29 ]. Apparently, the persistence of one or a few successful clones is unlikely, in contrast to A. baumannii [ 22 , 23 ].…”

Section: Discussionsupporting

confidence: 56%

Evolution of the Gram-Negative Antibiotic Resistance Spiral over Time: A Time-Series Analysis

et al. 2021

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We followed up the interplay between antibiotic use and resistance over time in a tertiary-care hospital in Hungary. Dynamic relationships between monthly time-series of antibiotic consumption data (defined daily doses per 100 bed-days) and of incidence densities of Gram-negative bacteria (Escherichia coli, Klebsiella spp., Pseudomonas aeruginosa, and Acinetobacter baumannii) resistant to cephalosporins or carbapenems were followed using vector autoregressive models sequentially built of time-series ending in 2015, 2016, 2017, 2018, and 2019. Relationships with Gram-negative bacteria as a group were fairly stable across years. At species level, association of cephalosporin use and cephalosporin resistance of E. coli was shown in 2015–2017, leading to increased carbapenem use in these years. Association of carbapenem use and carbapenem resistance, as well as of carbapenem resistance and colistin use in case of A. baumannii, were consistent throughout; associations in case of Klebsiella spp. were rarely found; associations in case of P. aeruginosa varied highly across years. This highlights the importance of temporal variations in the interplay between changes in selection pressure and occurrence of competing resistant species.

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Section: Discussionsupporting

confidence: 56%

Evolution of the Gram-Negative Antibiotic Resistance Spiral over Time: A Time-Series Analysis

et al. 2021

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“…Genetic relatedness among isolates was analysed by PFGE. Plugs were prepared as previously described (Mózes et al , 2014) and digested with Apa I. A CHEF DRIII machine (Bio-Rad) was used to separate the fragments in 1 % SeaKem Gold agarose (Lonza) at 14 °C.…”

Section: Methodsmentioning

confidence: 99%

Effect of carbapenem consumption patterns on the molecular epidemiology and carbapenem resistance of Acinetobacter baumannii

Mózes

Ebrahimi

Gorácz

et al. 2014

Journal of Medical Microbiology

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This study investigated the molecular epidemiology of Acinetobacter baumannii in the University of Debrecen in relation to antibiotic consumption. Overall and ward-specific antibiotic consumption was measured by the number of defined daily doses (DDD) per 100 bed-days between 2002 and 2012. Consumption was analysed against the number of A. baumannii positive patients per 100 bed-days, number of isolates per positive sample, and proportion of carbapenem resistant A. baumannii, using time-series analysis. Altogether 160 A. baumannii isolates from different wards were collected and analysed. Carbapenemase genes bla OXA-23-like , bla OXA-24-like , bla OXA-48-like , bla OXA-51-like , bla OXA-58-like and integrons were sought by PCR. Relatedness of isolates was assessed by PFGE. Prevalence and carbapenem resistance of A. baumannii were statistically associated with carbapenem consumption. Prevalence data followed carbapenem usage with three quarterly lags (r50.51-0.53, P,0.001), and meropenem and ertapenem, but not imipenem usage, affected prevalence. Colistin usage, in turn, lagged behind prevalence with one lag (r50.68-0.70, P,0.001). Six clusters were identified; the neurology ward with the lowest carbapenem consumption was associated with the carbapenem-susceptible cluster, as well as with the carbapenem-susceptible isolates in the cluster with variable susceptibility. Wards with high carbapenem usage almost exclusively harboured isolates from carbapenem-resistant clusters. All clusters were dominated by isolates of one or two wards, but most wards were represented in multiple clusters. Increases in prevalence and carbapenem resistance of A. baumannii were associated with usage of meropenem and ertapenem but not of imipenem, which led to the spread of multiple clones in the University.

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“…Plugs were prepared as described earlier [13], macrorestriction was performed using XbaI (Fermentas, Vilnius, Lithuania) in a CHEF DRIII machine (Bio-Rad) in 1% SeaKem Gold agarose (Lonza) at 14°C. Electrophoresis was performed at 6 V/cm, with a reorientation angle of 120°, and switch times were ramped between 2 and 64 s for 20 h for both species.…”

Section: Methodsmentioning

confidence: 99%

Carriage Rates and Characteristics of<b> </b>Enterobacteriaceae Producing Extended-Spectrum Beta-Lactamases in Healthy Individuals: Comparison of Applicants for Long-Term Care and Individuals Screened for Employment Purposes

Ebrahimi

Mózes²,

Mészáros³

et al. 2014

Chemotherapy

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Aims: We compared the prevalence of extended-spectrum beta-lactamase (ESBL) producers in the faecal samples of 1,109 healthy individuals screened for employment purposes and in 531 asymptomatic individuals applying for long-term care (LTC). Methods: Eosin-methylene blue agar plates supplemented with 2 mg/l cefotaxime were used to determine which individuals were ESBL producers. ESBL phenotype was confirmed by double-disk synergy test and ESBL genes were identified by sequencing. ESBL producers were characterized by co-resistance and integron carriage. Results: ESBL producers were more frequent in the LTC applicants than in the employment screening individuals (7.2 vs. 2.0%; p < 0.0001), with 43 Escherichia coli, 18 Klebsiella pneumoniae, 1 Klebsiella oxytoca and 1 Proteus mirabilis being found. In the employment screening individuals, only E. coli was found. Most ESBL genes (79.4%, 50/63) were bla_CTX-M type; bla_CTX-M-15 was more frequent in the LTC applicants (p < 0.001). Regarding ESBL genes and integron diversity, E. coli isolates from the LTC applicants were more similar to K. pneumoniae than to E. coli from the employment screening individuals. Conclusion: These differences in the characteristics of ESBL producers may represent different sources of colonization. Most LTC applicants harboured K. pneumoniae or E. coli that were probably hospital-acquired whereas the E. coli isolates of many healthy individuals showed similarities to environmental E. coli.

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A potential role of aminoglycoside resistance in endemic occurrence of Pseudomonas aeruginosa strains in lower airways of mechanically ventilated patients

Cited by 8 publications

References 29 publications

Evolution of the Gram-Negative Antibiotic Resistance Spiral over Time: A Time-Series Analysis

Evolution of the Gram-Negative Antibiotic Resistance Spiral over Time: A Time-Series Analysis

Effect of carbapenem consumption patterns on the molecular epidemiology and carbapenem resistance of Acinetobacter baumannii

Carriage Rates and Characteristics of<b> </b>Enterobacteriaceae Producing Extended-Spectrum Beta-Lactamases in Healthy Individuals: Comparison of Applicants for Long-Term Care and Individuals Screened for Employment Purposes

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