A potential probiotic Leuconostoc mesenteroides TBE-8 for honey bee

Abstract

An isolated bacterium TBE-8, was identified as Leuconostoc mesenteroides according to the sequences of 16S rDNA and the 16S–23S rDNA intergenic spacer region. The probiotic properties of the L. mesenteroides TBE-8 strain were characterized and revealed that TBE-8 could utilize various carbohydrates, exhibited high tolerance to sucrose’s osmotic pressure and acidic conditions, and could mitigate the impact of the bee pathogen Paenibacillus larvae. In addition, we found that the TBE-8 broth increased the expression of the nutrition-related genes major royal jelly protein 1 and vitellogenin in bees by approximately 1400- and 20-fold, respectively. The expression of genes encoding two antibacterial peptides, hymenoptaecin and apidaecin, in the bee abdomen was significantly increased by 17- and 7-fold in bees fed with the TBE-8 fermented broth. Furthermore, we fed four-frame bee colonies with 50% sucrose syrup containing TBE-8 and can detect the presence of approximately 2 × 106 16S rDNA copies of TBE-8 in the guts of all bees in 24 h, and the retention of TBE-8 in the bee gut for at least 5 days. These findings indicate that the L. mesenteroides TBE-8 has high potential as a bee probiotic and could enhance the health of bee colonies.

Investigation of lactic acid bacterial profiles in commercial rice wine and their effect on metabolites during low-temperature storage

“…The notified probiotic species in Korea, Lactobacillus helveticus and Lactobacillus plantarum , were detected in makgeolli samples in the present study. In addition, some strains of Enterococcus casseliflavus, Tetragenococcus halophilus, Lactobacillus delbrueckii, Lactobacillus paraplantarum, Lactobacillus zeae, Pediococcus acidilactici, Leuconostoc mesenteroides, Weissella hellenica, Weissella paramesenteroides , and Lactococcus garvieae , which were detected in this study, have been reported to possess probiotic potential ( Ayyash et al, 2020 , Barigela and Bhukya, 2021 , Huang et al, 2021 , Inayah et al, 2022 , Kim et al, 2022 , Pabari et al, 2020 , Panthee et al, 2019 , Safari et al, 2016 , Son et al, 2018 ). These species collectively accounted for 4.65% of the relative abundance of the makgeolli microbiome, and a total cell number of 7.15 log CFU per bottle of makgeolli.…”

A Novel Strain of Probiotic Leuconostoc citreum Inhibits Infection-Causing Bacterial Pathogens

“…However, our strains L. citreum KCC-57 and L. citreum KCC-58 survived significantly in lower pH (pH 2.5) without nutrition (acidic pH in PBS) compared to previously reported Leuconostoc spp. [ 41 , 42 ]. Furthermore, the resistant capacity of L. citreum KCC-57 and L. citreum KCC-58 was found to be higher in duodenal and intestinal juices compared to gastric juice.…”

Beneficial bacteria as biocontrol agents for American foulbrood disease in honey bees (Apis mellifera)

“…The exposure of honey bee larvae to LAB strains from the genus Lactobacillus spurred the immune response in larvae as evidenced by the up-regulated transcriptional expression of AMPs components, abaecin ( Evans and Lopez 2004 ) and Apidaecin 1 ( Janashia and Alaux 2016 ). The administration of Leuconostoc mesenteroides TBE-8 (Lactobacillales, Lactobacillaceae) (isolated from the hindgut of bumble bee B. eximius ) to honey bees significantly increased the transcriptional expression of nutrition-related genes (major royal jelly protein 1 in the head and vitellogenin in the abdomen) and AMPs genes (hymenoptaecin and apidaecin in the abdomen) ( Huang et al 2021 ). All the above mentioned modes of actions LAB exert on honey bees are summarized in Fig.…”