A potential probe set of fluorescence in situ hybridization for detection of lung cancer in bronchial brushing specimens

Liu, Yizhen; Wang, Zhen; Fang, Li-Li; Lu, Li; Cao, Jian; Xu, Xin; Han, Yaling; Cai, Yan; Wang, Liang-Xu; Wang, Ming-Rong

doi:10.1007/s00432-012-1232-0

Cited by 11 publications

(6 citation statements)

References 27 publications

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“…Seventeen studies with 2538 participants were retrieved 26‐42 . Figure 1 is a flowchart of the literature search, and Table 1 presents detailed characteristics of the studies.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the diagnostic accuracy of bronchial brushing cytology in lung cancer: A meta‐analysis

Chen

Bai

Lee

et al. 2021

Cancer Cytopathology

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Background Flexible bronchoscopy is commonly used to examine patients suspected to have lung cancer. Bronchial brushing is one of the cytological technologies for lung specimens obtained through a bronchoscope. However, the accuracy of bronchial brushing cytology (BBC) for lung cancer diagnosis is still inconclusive. The aim of this study was to evaluate the diagnostic accuracy of BBC. Methods A literature search was conducted with PubMed, Embase, the Cochrane Library, Web of Science, Biomed Central, Clinical Key, and ClinicalTrials.gov. Studies that assessed the efficacy of BBC in detecting lung cancer were included. Articles that estimated the accuracy on a per‐patient basis were included. Review articles, case reports, and research that provided insufficient data to construct a 2 × 2 table were excluded. Both prospective trials and retrospective studies were included. English language studies were reviewed. Data synthesis was performed with a random‐effects model. Results Seventeen studies with 2538 patients were included in the study. The meta‐analysis for BBC generated a pooled sensitivity of 0.67 (95% confidence interval [CI], 0.65‐0.70) and a pooled specificity of 0.91 (95% CI, 0.89‐0.93). The pooled diagnostic odds ratio for BBC was 24.55 (95% CI, 12.39‐48.66). The subgroup analysis for studies using liquid‐based cytology (LBC) generated a pooled sensitivity of 0.68 and a pooled specificity of 0.92. The pooled diagnostic odds ratio of studies using LBC was 114.18. Conclusions These findings indicate that BBC is a discriminative diagnostic approach with moderate sensitivity and high specificity for diagnosing peripheral pulmonary lesions. BBC using LBC has higher diagnostic performance.

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“…Seventeen studies with 2538 participants were retrieved 26‐42 . Figure 1 is a flowchart of the literature search, and Table 1 presents detailed characteristics of the studies.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the diagnostic accuracy of bronchial brushing cytology in lung cancer: A meta‐analysis

Chen

Bai

Lee

et al. 2021

Cancer Cytopathology

View full text Add to dashboard Cite

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“…They also found FISH for chromosomes 7, 11, 17, and 18 to be positive in bronchial brushings from 5 patients with lung cancer. operating margin tissue specimens without tumor and 174 bronchial brushing specimens [14]. Aneuploidy rates ranged from 62%-93% in tumor tissues and 29%-70% in bronchial brushings of lung cancer patients based on individual probe data.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Chromosome 3, 7 and 8 Centromeric Regions in Bronchial Lavage Specimens by FISH

Atasoy¹,

Erturan²,

Yilmaz³

et al. 2016

Turk Toraks Dergisi

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OBJECTIVES:Multiple genetic changes are observed in malignant tumors but are rare or absent in benign conditions. Aneuploidy is the most common feature of solid tumors including lung cancer and diagnosis of malignant tumors is possible through detection of aneuploidy. The aim of this study was to investigate chromosomal abnormalities in cells from non-small cell lung cancer patients obtained bronchoscopically and to evaluate the suitability of fluorescence in situ hybridization (FISH). MATERIAL AND METHODS:Bronchial lavage samples of 17 non-small cell lung cancer (NSCLC) patients were evaluated with fourcolor FISH using deoxyribonucleic acid (DNA) probes specific for the centromere regions of chromosomes 3, 7 and 8. tested specimens were first hybridized with probes, then visualized under fluorescence microscobeand captured with device's camera. RESULTS:High number of aneuploidic cells were detected in all the samples. Increased or decreased abnormal copies or chromosomes 3, 7 and 8 were obserced in all the 17 patients. Aneuploidy of chromosome 3 (21.35%) was higher than those of chromosome 7 (9.06%) and chromosome 8 (15.47%). Moreover, our results were significant for monosomy and trisomy of chromosome 3, trisomy of chromosome 7, nullisomy, monosomy and trisomy of, chromosome 8 (p< 0.05). CONCLUSION:It has been observed that FISH is a useful technique for detection of aneuploidy in bronchial lavage samples obtained by bronchoscopy. Interphase cells were evaluated without cell culturing with this method and high number of tumor cells were enumerated rapidly. Our study has demonstrated that, FISH technique may be used successfully in detection of chromosome number abnormalities in NSCLC patients and may facilitate evaluation of genetic abnormalities.

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“…The results were of evident clinical significance. [ 11 12 13 14 ] Since pleural effusion herein can be obtained easily, it is feasible to detect EGFR gene of the cell mass in the pleural effusion. Its amplification rate was 57.14% in malignant pleural effusion and the diagnosis sensitivity was 57.1%.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Values of Vascular Endothelial Growth Factor and Epidermal Growth Factor Receptor for Benign and Malignant Hydrothorax

Zhang

et al. 2015

Chinese Medical Journal

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Background:Hydrothorax, as one of the common complications of malignant tumors, still cannot be sensitively detected in clinical practice, thus requiring a sensitive, specific method for diagnosis. The aim of this study was to analyze the correlation between levels of vascular endothelial growth factor (VEGF) and epidermal growth factor receptor (EGFR) in patients with benign and malignant hydrothorax.Methods:The contents of VEGF in the pleural effusion and serum of the patients with malignant pleural effusion (n = 35) and benign pleural effusion (n = 30) were detected by double antibody sandwich enzyme linked immunosorbent assay. The gene copy number level of EGFR in pleural effusion was detected by fluorescence in situ hybridization (FISH). The points with the highest sensitivity and specificity were selected as the critical values to calculate the diagnostic value of the VEGF in pleural effusion and serum, and EGFR gene copy number in pleural effusion.Results:The contents of VEGF in pleural effusion and serum of patients with malignant hydrothorax were (384.91 ± 120.18), and (129.62 ± 46.35) ng/L, respectively, which were significantly higher than those of the patients with benign hydrothorax (207.97 ± 64.04), (63.49 ± 24.58) ng/L (P < 0.01). The sensitivity and specificity of detecting VEGF in pleural effusion were 80.0% and 96.7% (the boundary value was 297.06 ng/L), respectively for diagnosing benign and malignant hydrothorax. The sensitivity and specificity of serum were 74.3% and 96.7%, respectively (the boundary value was 99.21 ng/L) for diagnosing benign and malignant hydrothorax. The diagnostic efficiencies of EGFR and VEGF in hydrothorax were similar. There was a significant correlation between EGFR and VEGF in hydrothorax (P < 0.01).Conclusions:VEGF and EGFR play important roles in the formation of pleural effusion. VEGF differed significantly in benign and malignant pleural effusions, which contributed to differential diagnosis results of benign and malignant pleural effusions. It is feasible to detect the gene copy number of the pleural effusion cell mass EGFR by FISH technique. Joint detection can improve the diagnostic sensitivity.

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A potential probe set of fluorescence in situ hybridization for detection of lung cancer in bronchial brushing specimens

Abstract: FISH performed on cytology preparations is useful for confirmation of cancer diagnosis. The three-probe set, 3+7+8, has potential value for the detection of SCCs in bronchial brushings.

Cited by 11 publications

References 27 publications

Evaluation of the diagnostic accuracy of bronchial brushing cytology in lung cancer: A meta‐analysis

Evaluation of the diagnostic accuracy of bronchial brushing cytology in lung cancer: A meta‐analysis

Analysis of Chromosome 3, 7 and 8 Centromeric Regions in Bronchial Lavage Specimens by FISH

Diagnostic Values of Vascular Endothelial Growth Factor and Epidermal Growth Factor Receptor for Benign and Malignant Hydrothorax

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