A potent fluorescent biosensor integrating 3D DNA walker with localized catalytic hairpin assembly for highly sensitive and enzyme-free Zika virus detection

Liang, Guoxi; Ye, Shu-Ying; Yu, Haoming; Zhao, Kai-Ren; Liu, Pengfei; Liu, Zhijun; Wang, Li

doi:10.1016/j.snb.2021.131199

Cited by 14 publications

(6 citation statements)

References 43 publications

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“…A study conducted by Liang et al proposed a fluorescent biosensor that integrates the localized catalytic hairpin assembly (LCHA) cascade amplification strategy and a DNA walker as a novel ZIKV assay platform for rapid, accurate, and low-cost diagnosis [ 122 ]. The catalytic hairpin assembly (CHA) reaction is an isothermal amplification strategy that is useful for amplifying and transducing signals to detect DNA and RNA, and the LCHA reaction provides a faster reaction rate [ 123 ].…”

Section: Optical-based Detectionmentioning

confidence: 99%

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Recent Developments in DNA-Nanotechnology-Powered Biosensors for Zika/Dengue Virus Molecular Diagnostics

Park

et al. 2023

Nanomaterials

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Zika virus (ZIKV) and dengue virus (DENV) are highly contagious and lethal mosquito-borne viruses. Global warming is steadily increasing the probability of ZIKV and DENV infection, and accurate diagnosis is required to control viral infections worldwide. Recently, research on biosensors for the accurate diagnosis of ZIKV and DENV has been actively conducted. Moreover, biosensor research using DNA nanotechnology is also increasing, and has many advantages compared to the existing diagnostic methods, such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). As a bioreceptor, DNA can easily introduce a functional group at the 5′ or 3′ end, and can also be used as a folded structure, such as a DNA aptamer and DNAzyme. Instead of using ZIKV and DENV antibodies, a bioreceptor that specifically binds to viral proteins or nucleic acids has been fabricated and introduced using DNA nanotechnology. Technologies for detecting ZIKV and DENV can be broadly divided into electrochemical, electrical, and optical. In this review, advances in DNA-nanotechnology-based ZIKV and DENV detection biosensors are discussed.

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Section: Optical-based Detectionmentioning

confidence: 99%

“…( D ) Changes in the FL spectrum of ZIKV according to the concentration of the fabricated biosensor. Reproduced with permission from [ 122 ], published by Elsevier, 2022. ( E ) Schematic illustration of the fabricated DENV detection fluorescence aptasensor.…”

Section: Optical-based Detectionmentioning

confidence: 99%

Recent Developments in DNA-Nanotechnology-Powered Biosensors for Zika/Dengue Virus Molecular Diagnostics

Park

et al. 2023

Nanomaterials

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“…5B). 89 The interaction between the target and locked walking strands initiates the autonomous motion of the walking strands along the hairpin tracks, cleaving the track DNAs into two segments. The released segments initiate the two neck rings on the tetrahedron to open successively and form a stable double-chain structure, which is accompanied by the release of a fluorescence signal.…”

Section: D Walkers Coupled With Diverse Isothermal Amplification Tech...mentioning

confidence: 99%

The rate-limiting procedure of 3D DNA walkers and their applications in tandem technology

Chen

et al. 2023

Chem. Commun.

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“…Wang and colleagues designed DNA walker‐based fluorescent biosensor with localized CHA (LCHA) for detecting Zika viruses (ZIKV). [ 103 ] In this work, the DNA walker and hairpin DNA tracks were immobilized on the AuNPs. And the DNA walker was locked on the other end.…”

Section: Dna Walkers‐based Biosensorsmentioning

confidence: 99%

DNA Walkers for Biosensing Development

et al. 2022

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The ability to design nanostructures with arbitrary shapes and controllable motions has made DNA nanomaterials used widely to construct diverse nanomachines with various structures and functions. The DNA nanostructures exhibit excellent properties, including programmability, stability, biocompatibility, and can be modified with different functional groups. Among these nanoscale architectures, DNA walker is one of the most popular nanodevices with ingenious design and flexible function. In the past several years, DNA walkers have made amazing progress ranging from structural design to biological applications including constructing biosensors for the detection of cancer‐associated biomarkers. In this review, the key driving forces of DNA walkers are first summarized. Then, the DNA walkers with different numbers of legs are introduced. Furthermore, the biosensing applications of DNA walkers including the detection‐ of nucleic acids, proteins, ions, and bacteria are summarized. Finally, the new frontiers and opportunities for developing DNA walker‐based biosensors are discussed.

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A potent fluorescent biosensor integrating 3D DNA walker with localized catalytic hairpin assembly for highly sensitive and enzyme-free Zika virus detection

Cited by 14 publications

References 43 publications

Recent Developments in DNA-Nanotechnology-Powered Biosensors for Zika/Dengue Virus Molecular Diagnostics

Recent Developments in DNA-Nanotechnology-Powered Biosensors for Zika/Dengue Virus Molecular Diagnostics

The rate-limiting procedure of 3D DNA walkers and their applications in tandem technology

DNA Walkers for Biosensing Development

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