A Potent Antipoliomyelitic Horse Serum Concentrate and Its Experimental Use in Infected Monkeys

Abstract

Since the discovery b y N e t t e r (1) and Levaditi (2) of the presence of virus-neutralizing antibodies in the blood, both from human convalescents and from monkeys recovered from experimental poliomyelitis, several attempts have been made to immunize large animals with a view to quantity production of therapeutic serum.

Study of the Serum-Neutralization Test in Poliomyelitis

“…Only one method of antibody purification has hitherto been used in poliomyelitis work. Weyer, Park and Banzhaf (1931), and Morgan and Fairbrother (1930) employed the salt fractionation technique to concentrate and purify, to some extent, the antibodies of hyperimmunized horse serum. Since these two groups of workers disagree as to what protein fraction of the serum, pseudoglobulin or euglobulin, contains the higher concentration of antibody, and since the method was for other reasons unsuited to this investigation, our attempts at antibody purification have been along other lines.…”