A possible theranostic approach of chitosan-coated iron oxide nanoparticles against human colorectal carcinoma (HCT-116) cell line

Abstract

Iron oxides have become increasingly popular for their use as a diagnostic and therapeutic tool in oncology. This study aimed to improve pharmacological valuable of Fe 3 O 4 , which may be use to diagnosis colorectal cancers (CRC). Here, we have developed chitosan (CS) coated Fe 3 O 4 through a cost-effective procedure. First, we determined the characterization of OA-C-Fe 3 O 4 by FTIR, UV–Vis spectra, and TEM. Then, we evaluated the photodynamic therapeutic (PDT) activity of OA-C-Fe 3 O 4 in human colorectal carcinoma cell lines (HCT 116). Current results revealed that the light-induced enhanced reactive oxygen species (ROS) activity of the nanoparticles (NPs) and caused cell death via the activity of caspase 9/3. The in vitro magnetic resonance imaging (MRI) experiments in (HCT 116) and human embryonic kidney cells (HEK 293) illustrated that nanohybrid is an effective MRI contrasting agents for the diagnosis of colorectal cancer.

Using magnetic mesoporous silica nanoparticles armed with EpCAM aptamer as an efficient platform for specific delivery of 5-fluorouracil to colorectal cancer cells

“…Similarly, MRI results demonstrated the increased lesion localization and tumor affinity of Apt-PEG-Au-NPs@5-FU in comparison with PEG-Au-NPs@5-FU after 12 h. These results support the notion that targeted nanocarriers can be used as a preferred theranostic platform for effective CRC inhibition. This finding is consistent with the results reported by Alkahtane et al which illustrated that chitosan coated Fe 3 O 4 nanohybrid enhanced MRI contrast ability for CRC therapy (Alkahtane et al, 2022). Moreover, Zou et al reported that MUC1-SPIONs as a targeted construct can effectively improve pancreatic tumor-targeting as confirmed by both in vitro and in vivo MRI studies (Zou et al, 2019).…”

Cytotoxicity Mechanisms of Blue-Light-Activated Curcumin in T98G Cell Line: Inducing Apoptosis through ROS-Dependent Downregulation of MMP Pathways

“…T98G and LN229 cells were cultured in DMEM, which was supplemented with 10% FBS and 1% PSN antibiotic under humidified conditions (5% CO 2 ). The detailed procedure of cell culture and MTT assay adapted to the standard methods and practices [ 71 ], was also implemented in our previous work [ 72 , 73 ]. Here, the concentrations of Curcumin were maintained at 0–25 µM for the treatment of the glioblastoma cell line for 24 h.…”

Polysaccharide-Based Theranostic Systems for Combined Imaging and Cancer Therapy: Recent Advances and Challenges

“…Further, Gholipour et al developed a 68 Ga-dextran complex (GaD) for dual-modality PET/MRI imaging. The efficacy of the designed complex was tested under both acidic and alkaline conditions.…”

“…213 Even though the small amounts of radiopharmaceuticals can provide activities sufficient for acquisition of images, a stable chelation is required in the case of metallic tracers to ensure their safe clinical application. For example, complexation of a common PET radioisotope 68 Ga 3+ by suitable small-molecular ligands (DOTA/NOTA) can prevent its binding to transferrin in vivo as the result of mimicking Fe 3+ . 214 For some cations with a more complicated coordination core, such as a positron emitter 89 Zr 4+ (t 1/2 = 78.4h), the classic coordination yields a complex whose stability is not sufficient to endure the long exposure to the in vivo environment.…”