A possible role of the cholinergic and purinergic receptor interaction in the regulation of the rat urinary bladder function

Jenes, Ágnes; Ruzsnavszky, Ferenc; Telek, Andrea; Szigeti, Gyula P.; Csernoch, László

doi:10.1007/s10974-012-9285-x

Cited by 4 publications

(7 citation statements)

References 57 publications

(49 reference statements)

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“…The cross-inhibition was more prominent in the base of the bladder. Similar cross-interaction was already described in rat smooth muscle cells from the base of the urinary bladder, when the smooth muscle cells were isolated from 8-12 weeks old animals (Jenes et al 2012). …”

Section: Discussionsupporting

confidence: 80%

“…Similar interaction between nicotinic and purinergic receptors in rat urinary bladder smooth muscle has been recently described (Jenes et al 2012).…”

Section: Introductionsupporting

confidence: 72%

“…It must be noted that although our immunohistochemistry data suggested the presence of α 7 nicotinic receptor subunits in human bladders, it is unlikely that this subunit alone is responsible for mediating the effects of cholinergic stimulation, as it was reported in rat urinary bladders (Jenes et al 2012). The possible roles of other nicotinic receptor subunits are suggested by the marked difference between the cholinergic agonists that are capable of interfering with purinergic stimulation in rats and humans.…”

Section: Cholinergic and Purinergic Receptor Activation Of Human Urinsupporting

confidence: 45%

“…Smooth muscle cells were cultured as described previously (Jenes et al 2012), with an increased collagenase concentration to 1 mg/ml. Following removal of the mucosal layer the muscle piece was placed into trypsin (0.75 mg/ml) -EDTA (0.4 mg/ml) solution for 30 minutes at 37°C.…”

Section: Human Urinary Bladder Smooth Muscle Cell Culturementioning

confidence: 99%

“…Changes in the concentration of [Ca 2+ ] i were detected using the fluorescent dye Fura-2 as described in our previous reports (Bíró et al 1998;Csernoch et al 2000;Jenes et al 2012). The coverslips with the Fura-2 loaded cells (90 min, 37°C, 15 µM Fura-2 AM) were placed on the stage of an inverted fluorescence microscope (Diaphot, Nikon, Japan).…”

Section: Fluorescent Measurement Of [Ca 2+ ] Imentioning

confidence: 99%

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Nicotine interferes with purinergic signaling in smooth muscle cells isolated from urinary bladders of patients with lower urinary tract symptoms

Jenes¹,

Szigeti²,

Ruzsnavszky³

et al. 2014

gpb

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Abstract. In patients with outlet obstruction, the contraction of the base is reduced compared to that of healthy individuals, while the contraction of the dome is not affected. Here, we investigated the cellular mechanisms that might be responsible for cholinergic effects blocking non-adrenergic non-cholinergic contractions in the base of the urinary bladder.Smooth muscle cells either from the base or from the dome of human urinary bladders were cultured to determine the contribution of cholinergic and purinergic mechanisms to their Ca 2+ homeostasis.While ATP evoked Ca 2+ transients in all the cells, nicotine and carbachol induced Ca 2+ transients only in 56% and 44% of the cells, respectively. When ATP was administered together with nicotine or carbachol, the amplitudes of the Ca 2+ transients recorded from cells prepared from the base of bladders were significantly smaller (42 ± 6% with nicotine and 56 ± 9% with carbachol) than those evoked by ATP alone. This inhibition was much less apparent in the dome of bladders.The inhibition between the cholinergic and purinergic signaling pathways reported in this work may decrease the strength of the contraction of the base of the urinary bladder in patients with outlet obstruction during voiding.

show abstract

Section: Discussionsupporting

confidence: 80%

“…Similar interaction between nicotinic and purinergic receptors in rat urinary bladder smooth muscle has been recently described (Jenes et al 2012).…”

Section: Introductionsupporting

confidence: 72%

Section: Cholinergic and Purinergic Receptor Activation Of Human Urinsupporting

confidence: 45%

Section: Human Urinary Bladder Smooth Muscle Cell Culturementioning

confidence: 99%

Section: Fluorescent Measurement Of [Ca 2+ ] Imentioning

confidence: 99%

See 3 more Smart Citations

Nicotine interferes with purinergic signaling in smooth muscle cells isolated from urinary bladders of patients with lower urinary tract symptoms

Jenes¹,

Szigeti²,

Ruzsnavszky³

et al. 2014

gpb

Self Cite

View full text Add to dashboard Cite

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Purinergic signalling in the urinary tract in health and disease

Burnstock

2013

Purinergic Signalling

140

149

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Purinergic signalling is involved in a number of physiological and pathophysiological activities in the lower urinary tract. In the bladder of laboratory animals there is parasympathetic excitatory cotransmission with the purinergic and cholinergic components being approximately equal, acting via P2X1 and muscarinic receptors, respectively. Purinergic mechanosensory transduction occurs where ATP, released from urothelial cells during distension of bladder and ureter, acts on P2X3 and P2X2/3 receptors on suburothelial sensory nerves to initiate the voiding reflex, via low threshold fibres, and nociception, via high threshold fibres. In human bladder t h e p u r i n e rg i c c o m p o n e n t o f p a r a s y m p a t h e t i c cotransmission is less than 3 %, but in pathological conditions, such as interstitial cystitis, obstructed and neuropathic bladder, the purinergic component is increased to 40 %. Other pathological conditions of the bladder have been shown to involve purinoceptor-mediated activities, including multiple sclerosis, ischaemia, diabetes, cancer and bacterial infections. In the ureter, P2X7 receptors have been implicated in inflammation and fibrosis. Purinergic therapeutic strategies are being explored that hopefully will be developed and bring benefit and relief to many patients with urinary tract disorders.

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Bifidobacterium infantis-mediated HSV-TK/GCV suicide gene therapy induces both extrinsic and intrinsic apoptosis in a rat model of bladder cancer

Yin

Tang

et al. 2012

Cancer Gene Ther

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Bladder cancer is the most common cancer in the urological tract. Here, we investigated the molecular pathways involved in the apoptosis of rodent bladder cancer, which was treated with Bifidobacterium infantis (BI)-mediated herpes simplex virus thymidine kinase (HSV-TK)/ganciclovir (GCV) suicide gene therapy. We engineered the BI-TK system, which consists of BI with the recombinant plasmid PGEX-1λT carrying HSV-TK (that is, TK) gene. Tumor-bearing rats were randomly divided into three groups and tail vein injected with normal saline (group A), BI/PGEX-1λT (group B) or BI-TK (group C), followed by intraperitoneal injection of GCV. The BI-TK/GCV system (group C) was shown to inhibit tumor growth and caused the apoptosis of tumor cells as assessed by in situ terminal transferase dUTP nick-end labeling assay. While varied extents of apoptosis were detected in tumor cells from all groups, tumor cells treated with BI-TK/GCV system (group C) exhibited the highest level of apoptosis (P < 0.05), consistent with our previous studies. Furthermore, we found that the expression levels of Fas, FasL, Cyt-C and caspase-9 in tumor tissues derived from group C were significantly higher than the other two groups (P < 0.001). Therefore, our results have demonstrated that the BI-TK/GCV therapy system exhibits a sustainable antitumor growth activity in the rodent model of bladder cancer. Mechanistically, both extrinsic and intrinsic apoptosis pathways are involved in the BI-TK/GCV antitumor functionality.

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A possible role of the cholinergic and purinergic receptor interaction in the regulation of the rat urinary bladder function

Cited by 4 publications

References 57 publications

Nicotine interferes with purinergic signaling in smooth muscle cells isolated from urinary bladders of patients with lower urinary tract symptoms

Nicotine interferes with purinergic signaling in smooth muscle cells isolated from urinary bladders of patients with lower urinary tract symptoms

Purinergic signalling in the urinary tract in health and disease

Bifidobacterium infantis-mediated HSV-TK/GCV suicide gene therapy induces both extrinsic and intrinsic apoptosis in a rat model of bladder cancer

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